Effectiveness of Maceration Periods with Different Extraction Solvents on in-vitro Antimicrobial Activity from Fruit of Momordica charantia L.

Yeo, Yang Lin; Chia, Yin Yin; Lee, Chin Hong; Sow, Heng Sheng; Yap, Wai Sum

doi:10.7324/japs.2014.401004

Cited by 44 publications

(39 citation statements)

References 33 publications

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“…However, our findings in a previous study show that aqueous extracts have not shown any antibacterial activity due to the influence of solvents nature and polarity. For this reason, ethanol extracts of the seventeen most common plants cited by the healers (FI≥8.88%) have been prepared in accordance to the methods described by Elaloui et al and Yeo et al with slight modifications [17,18]. The used part of each plant as mentioned by herbalists was grounded to powder, and then 10 g of the powder of each plant was macerated in 100 ml of ethanol under agitation (500 rpm), at room temperature for 6 h. The resulting mixture was filtered through Whatman filter n°1 then evaporated under vacuum.…”

Section: Plants Selection and Preparationmentioning

confidence: 99%

In Vitro Antibacterial Activity of Medicinal Plants in the Central North of Morocco: A Possible Source of Alternative Drugs Against Methicillin-Resistant Staphylococcus Aureus

Zeouk

Lalami

Bekhti

2019

Asian J Pharm Clin Res

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Objective: The present study aims the investigation of the antimicrobial potential of medicinal plants selected in the central north of Morocco against methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis strain often involved in dermatitis. Methods: Structured interviews were carried out among 91 herbalists and traditional healers through a specific information questionnaire, the in vitro susceptibility of Staphylococcus strains award ethanol extracts was evaluated using the well-diffusion assay, while the agar-microdilution method was used to determinate the minimal inhibitory concentrations (MIC). The total phenolic and flavonoids contents of all tested extracts were also determined. Results: Based on the ethnobotanical survey, a total of 55 plant species belonging to 30 families were mentioned. The Lamiaceae family was the most represented (18.80%) followed by the Apiaceae family (10.90%). Leaves (45.00%) were the favored used part. Decoction method (48.53%) was the most frequently used to prepare remedies that are taken externally (75.00%). Nine of the 17 most selected species have shown an effective antistaphylococcal activity; the most active extracts were Punica granatum and Rhamnus alaternus with MIC values ranging between 0.25 mg/ml and 2.00 mg/ml. Conclusion: The current data confirm the good antistaphylococcal activity of P. granatum and R. alaternus and suggest that these species could constitute a promoter source for antistaphylococcal drugs with deeply studies.

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Section: Plants Selection and Preparationmentioning

confidence: 99%

In Vitro Antibacterial Activity of Medicinal Plants in the Central North of Morocco: A Possible Source of Alternative Drugs Against Methicillin-Resistant Staphylococcus Aureus

Zeouk

Lalami

Bekhti

2019

Asian J Pharm Clin Res

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“…The antibacterial activity was performed by the method of [21]. The broth culture of the test bacteria was maintained at 10 6 cells /ml using hemocytometer (Quijing-China) The bacterial cultures (100 µL) were separately incubated at 37°C for 24 h to observe the colonies later on a small portion of colony was emulsified in nutrient broth and kept for 30 minutes to activate the test culture.…”

Section: Antibacterial Activity Of Test Extracts Disc Diffusion Methodsmentioning

confidence: 99%

Antibacterial activity of Momordica charantia L. and Citrus limon L. on gram positive and gram negative bacteria

Ahmed

Noor²

2020

PAB

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The uses of herbal medicines are well recognized since the advent of mankind having very little side effects. Their use is a cheaper and quite affordable source of antimicrobial particularly in the rural areas. Some of the microbial strains are drug resistant and are always been a threat to human life. The herbal therapy is the best choice of remedy against the drug-resistant strains, which had opened the new roads for conventional use due to several antimicrobial phytochemicals and essential oils. Momordica charantia Linnaeus (L). and Citrus limon L. contain phytochemicals, which affect the bacteria, fungi, viruses, and parasites The aim of this study is to examine the effects of Momordica charantia and Citrus limon peel extracts on gram-positive and gramnegative bacteria. Preparation of extracts in ethanol, methanol, and ether were prepared and activation of test cultures, antimicrobial assay by disc diffusion, well diffusion and spectrophotometry at 600 nm. The greater zones of inhibition were determined by ethanolic extract of Momordica charantia on the growth of Bacillus cereus and Pseudomonas aeruginosa whereas Citrus limon methanolic peel extract revealed the greater effects on Staphylococcus aureus, Streptococcus pyogenes, and Escherichia coli at 15 and 20 µl concentrations respectively. The observations concluded that both the plant extracts have effects on bacteria that indicate that the preparations of these extracts up to pharmaceutical standard may be effectively used as antibacterial therapy against gram-positive and gram-negative bacteria.

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“…Marlboro fusion, (6) Marlboro gold, (7) Marlboro Xpress, (8) Lucky Strike, (9) Chesterfield, and (10) Philip Morris (of Argentine origin). 3 g of each variety of cigarette was subjected to consecutive extraction with petroleum ether, dichloromethane, and ethanol by cold maceration in an orbital shaker at 114 rpm for 120 hours [8]. e extracts were concentrated by using a rotavapor (Buchi R-111) at a maximum temperature of 40°C.…”

Section: In-layer Chromatographymentioning

confidence: 99%

Differentiation ofCandida albicansSpecies Complex by Tobacco Agar Obtained from Different Cigarette Brands Available in Colombia

Morales-López

Yepes

Elles

et al. 2020

International Journal of Microbiology

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Introduction. The Candida albicans complex is formed by Candida albicans, Candida dubliniensis, and a biovar of C. albicans named Candida africana. These yeasts are recognized as globally distributed clinical pathogens and share most phenotypic characteristics, which makes their discrimination by conventional methods difficult. Aim. To evaluate the efficacy of different brands of cigarettes in the preparation of tobacco agar, for the differentiation of these related yeasts. Methodology. Tobacco agar was prepared using six brands and four varieties of cigarettes, and 125 clinical isolates previously identified by PCR and Maldi-Tof were used. To determine whether the results of the microbiological tests were associated with similarities in the chemical components of cigarettes, thin-layer chromatography was performed. Results. Candida dubliniensis colonies presented hue differences according to the incubation temperature and the brand or variety of cigarette used, except in the tobacco agar produced with Marlboro Xpress cigarette, where its differentiation was not possible. The chromatograms showed few differences among apolar and medium polarity extract components. Conclusions. Tobacco agar is a low-cost tool used for the differentiation of Candida dubliniensis; however, incubation temperature and cigarette brand affect the performance of the media. No relationship was found between the microbiological results and the chemical similarity of the extracts of the cigarettes by chromatography.

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Effectiveness of Maceration Periods with Different Extraction Solvents on in-vitro Antimicrobial Activity from Fruit of Momordica charantia L.

Cited by 44 publications

References 33 publications

In Vitro Antibacterial Activity of Medicinal Plants in the Central North of Morocco: A Possible Source of Alternative Drugs Against Methicillin-Resistant Staphylococcus Aureus

In Vitro Antibacterial Activity of Medicinal Plants in the Central North of Morocco: A Possible Source of Alternative Drugs Against Methicillin-Resistant Staphylococcus Aureus

Antibacterial activity of Momordica charantia L. and Citrus limon L. on gram positive and gram negative bacteria

Differentiation ofCandida albicansSpecies Complex by Tobacco Agar Obtained from Different Cigarette Brands Available in Colombia

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