Effectiveness of Ethanolic Extract Ketepeng cina Leaves (Senna alata L.) As Antidiabetic Activity Test In Male Wistar Rats Induced by Alloxan

Abstract

Antidiabetic activity test of ethanolic extract of ketepeng cina leaves (Senna alata L.) to rats induced by alloxan has been done. Male wistar albino rats weree used as animal models which divided into 6 groups, normal group (aquadest), negative control group (Sodium CMC 0,5%), positive control group (insulin by 1IU/kgBW), and 3 test group (ethanolic extract of ketepeng cina leaves 200, 400, dan 800 mg/kgBB). Rats blood glucose level after induced intraperitoneally by alloxan 130 mg/kgBB can be stated as diabetic when >200 mg/dL. Antidiabetic-activity was tested by measuring on blood sugar levels by using the DTN-410-K fotometer, on day 0, 5, 10, and 15. The result of AUC0-15 and percentage of decreasing blood sugar levels for positive control group are 2557,35 and 51,20%, and 3 treatment groups (200 mg/kgBW, 400 mg/kgBW, and 400 mg/kgBW) 2745,6 and 39,94%; 2618,55 and 47,54%; 2448,425 and 57,72%. This point indicated that the ethanolic extract of ketepeng cina leaves has an antidiabetic activity and there is no significant difference compared with insulin (p<0,05). 800 mg/kgBW dose is the effectivity dose from other treatment doses, because it had the lowest AUC0-15 and the highest perscentage of decreasing blood sugar levels. According to the relation between percentage of blood glucose decrease level with dose, value of ED50 of ethanolic extract of ketepeng cina leaves is 522.413 mg/kgBW.