Effectiveness of Aureobasidium pullulans and Candida oleophila against postharvest strawberry rots

Lima, Giuseppe; Ippolito, Antonio; Nigro, Franco; Salerno, M.

doi:10.1016/s0925-5214(96)01302-6

Cited by 161 publications

(65 citation statements)

References 18 publications

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“…Although several possible mechanisms have been reported (Droby and Chalutz, 1994;Janisiewicz and Korsten, 2002;Massart and Jijakli, 2007), the main mode of action of yeast(-like) biocontrol agents is believed to be competition for nutrients and space (Droby and Chalutz, 1994;Lima et al, 1997;Janisiewicz et al, 2000;Castoria et al, 2001). According to the literature dealing with competition for nutrients and space using different antagonist/pathogen(/host) systems (Castoria et al, 1997(Castoria et al, , 2001Lima et al, 1997;Janisiewicz et al, 2000;Guetsky et al, 2002;Vero et al, 2002): (i) most studies have considered either the in vitro or the in situ approach but not both, (ii) the contribution of the competition for nutrients and competition for space has rarely been considered separately, and (iii) in situ assays have been often performed using the Nutrient Yeast Dextrose Broth medium whose composition is not comparable with that of host tissues. In the present study, competition for nutrients as a mode of action of A. pullulans strain Ach1-1 against P. expansum was first examined in vitro using a system that allows discrimination between competition for nutrients and that for space (Janisiewicz et al, 2000) using increasing concentrations of apple juice.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

Bencheqroun

Bajji

Massart

et al. 2007

Postharvest Biology and Technology

128

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Aureobasidium pullulans strain Ach1-1 was selected for its effectiveness against blue mold caused by Penicillium expansum on stored apple fruit. The possible involvement of competition for nutrients in the biocontrol activity of this antagonistic strain was investigated both in vitro and in situ. For in vitro assays, the effect of strain Ach1-1 on germination percentages of P. expansum conidia was evaluated after a 24 h incubation period in the presence of increasing apple juice concentrations (0-5%) using a system allowing the physical separation of both agents. In the absence of strain Ach1-1, conidial germination was strongly promoted by apple juice whatever the concentration. However, germination was significantly reduced by the presence of strain Ach1-1 except at the highest juice concentration. For conidia previously inhibited at 0.5% juice, germination after 24 h of incubation was partially recovered in the presence of strain Ach1-1 when fresh juice was added to a final concentration of 5%, and completely restored at both 0.5 and 5% juice concentrations in the absence of strain Ach1-1. For in situ assays, strain Ach1-1 was very protective against P. expansum on postharvest wounded apples. However, the application of high concentrations of exogenous sugars, vitamins and most particularly amino acids, significantly reduced such protection. Time-course analysis of apple amino acids at the wound site revealed that these compounds were more depleted in wounds treated with strain Ach1-1 alone and especially in those treated with both agents (strain Ach1-1 and P. expansum) compared to wounds treated with P. expansum alone or to untreated ones. Exogenous amino acids, applied at high concentrations on apple wounds as a mixture of specific amino acid groups or as individuals, significantly decreased strain Ach1-1 efficacy against P. expansum. The present study provides in vitro and in situ evidence that competition for apple nutrients, most particularly amino acids, may be a main mechanism of the biocontrol activity of A. pullulans strain Ach1-1 against blue mold caused by P. expansum on harvested apple fruit.

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Section: Introductionmentioning

confidence: 99%

In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

Bencheqroun

Bajji

Massart

et al. 2007

Postharvest Biology and Technology

128

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“…The yeast-like fungus Aureobasidium pullulans (de Bary) Arnaud is a wide-spread saprophyte in the phyllosphere that has been considered as an effective biocontrol agent against post-harvest diseases (Lima et al 1997;Schena et al 1999;Ippolito et al 2000;Castoria et al 2001). Recently, A. pullulans strain Ach 1-1 was isolated from the surface of apple fruit in Morocco and previously selected for its high antagonistic activity against Penicillium expansum and Botrytis cinerea (Achbani et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Production, formulation and antagonistic activity of the biocontrol like-yeast Aureobasidium pullulans against Penicillium expansum

et al. 2007

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Aureobasidium pullulans (de Bary) Arnaud (Ach 1-1) was grown in a glucose fed-batch fermentor to 106 g dry wt l -1 in 48 h. The cells were dried in a fluidized bed dryer with a final viability of 62%. After 7 months at 4°C, the viability was 28% of the initial value (= 2.3 · 10 10 c.f.u. g -1 dry matter). A protection level of 89% was achieved with the biomass preparation at 1 · 10 8 c.f.u. ml -1 after 28 and 7 days for apples stored respectively at 5 and 25°C against Penicillium expansum. Our process is suitable to produce large quantities of the strain Ach 1-1 as biological control agent for apple preservation.

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“…Vuill and Aspergillus niger Tiegh on table grapes (Castoria et al 2001), and the ochratoxigenic Aspergillus carbonarius (Bainier) Thom. on table grapes and strawberry (Lima et al 1997;Castoria et al 2001;De Felice et al 2008;De Curtis et al 2012b), against B. cinerea and P. expansum on apples (Castoria et al 2001;Ippolito et al 2000;Mounir et al 2007), against three species of the genus Monilinia on stone fruit (Mari et al 2012). De Felice et al (2008) observed for the first time that three strains of A. pullulans were the effective biocontrol agents in reducing both severity of Aspergillus rots in the vineyard and ochratoxin A (OTA) accumulation in wine grapes.…”

Section: Introductionmentioning

confidence: 99%

Antagonistic interactions between Aureobasidium pullulans and Fusarium culmorum, a fungal pathogen of winter wheat

Wachowska

Głowacka

2014

BioControl

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The effect of the biological control agent Aureobasidium pullulans (de Bary) G. Arnaud on the development of Fusarium head blight (FHB) on winter wheat and kernel contamination with fungi of the genera Fusarium, Acremonium, Cladosporium and Penicillium was analyzed in a greenhouse experiment. Scanning electron microscopy was used to evaluate the distribution of A. pullulans cells and aggregates on wheat kernels, infection structures of Fusarium culmorum (W.G. Smith) Sacc and the antagonist-pathogen interactions. Biological control with A. pullulans reduced FHB severity by 21.67 % and improved grain filling by 5.02 %, compared with the control treatment. The survival of A. pullulans was good (to 31 cells per kernel), in particular on the surface and in the crease of kernels, including in pathogen-inoculated wheat plants. A. pullulans cells firmly adhered to F. culmorum hyphae, and damaged them. In most cases, autochthonous communities of filamentous fungi of the genera Acremonium and Penicillium developed at a slower rate after kernel inoculation with the pathogen.

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Effectiveness of Aureobasidium pullulans and Candida oleophila against postharvest strawberry rots

Cited by 161 publications

References 18 publications

In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

In vitro and in situ study of postharvest apple blue mold biocontrol by Aureobasidium pullulans: Evidence for the involvement of competition for nutrients

Production, formulation and antagonistic activity of the biocontrol like-yeast Aureobasidium pullulans against Penicillium expansum

Antagonistic interactions between Aureobasidium pullulans and Fusarium culmorum, a fungal pathogen of winter wheat

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