Production, formulation and antagonistic activity of the biocontrol like-yeast Aureobasidium pullulans against Penicillium expansum

Mounir, R.; Durieux, Alain; Bodo, E.; Allard, C.; Simon, Jean Paul; Achbani, El Hassan; El-Jaafari, S.; Douira, Allal; Jijakli, M. Haïssam

doi:10.1007/s10529-006-9269-2

Cited by 33 publications

(28 citation statements)

References 10 publications

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“…Among these amino acids, glycine, glutamic acid and especially serine appear to be the most limited nutrients in this mechanism. In a recent study (Mounir et al, 2007), it was found that strain Ach 1-1 was very effective in controlling blue mould on apples at both 5 • C and 25 • C and that biomass production in a fermentor (fed-batch) as well as drying (fluidized bed) did not affect its antagonistic activity. As new potential biocontrol agents, the specific identification of strains Ach 1-1 and 1113-5 using monitoring tools is needed for their practical application and for their commercial use.…”

Section: Discussionmentioning

confidence: 98%

“…doi: 10.1016/j.postharvbio.2008.05.011 In this context, A. pullulans strains Ach 1-1 and 1113-5 were isolated from 'Golden Delicious' apples for their high antagonistic activity (more than 80%) against B. cinerea and P. expansum, two major postharvest pathogens of apples (Achbani et al, 2005). With regard to strain Ach 1-1, a process suitable for producing large quantities has been developed (Mounir et al, 2007) and competition for apple nutrients, most particularly amino acids, may be the main mechanism of its biocontrol activity against blue mold caused by P. expansum on harvested apple fruit (Krimi Bencheqroun et al, 2007). The practical application of Ach 1-1 and 1113-5 strains as biocontrol agents will require the development of monitoring methods allowing their specific identification and their quantification.…”

Section: Introductionmentioning

confidence: 99%

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Development of SCAR markers and a semi-selective medium for the quantification of strains Ach 1-1 and 1113-5, two Aureobasidium pullulans potential biocontrol agents

Hamouchi

Bajji

Friel

et al. 2008

Postharvest Biology and Technology

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a b s t r a c tAureobasidium pullulans strains Ach 1-1 and 1113-5 are two effective biocontrol agents against Botrytis cinerea and Penicillium expansum on stored apples. In the present work, a monitoring system allowing their identification and quantification was developed. The methodology used consisted of the development of both molecular markers and a semi-selective medium. The random amplified polymorphic DNA (RAPD) technique was applied to a collection of 15 strains of A. pullulans, including Ach 1-1 and 1113-5. Five specific RAPD fragments were amplified for strain Ach 1-1 and three others for strain 1113-5. Among them, a fragment of 528 bp specific to strain Ach 1-1 (generated with the OPR-13 RAPD primer) and another one of 431 bp specific to strain 1113-5 (amplified with the OPQ-03 RAPD primer) were selected, cloned, sequenced, and used to design sequence-characterized amplified region (SCAR) primers. Three different SCAR markers were amplified: two specific to strain Ach 1-1 (189 bp and 387 bp) and one specific to strain 1113-5 (431 bp). These SCAR primers can clearly identify strains Ach 1-1 and 1113-5 among 14 strains of A. pullulans and among eight yeast strains commonly present on apple fruit surfaces. Their selectivity was also tested using DNA extracted from epiphytic microflora of the apple surface. As a semi-selective medium, PDA medium supplemented with 0.5 mg L −1 euparen, 1 mg L −1 sumico, 2.5 mg L −1 hygromycin B, 30 mg L −1 streptomycin sulphate, and 1 mg L −1 cycloheximide was selected. It inhibited the development of the air microflora and appeared highly toxic for the epiphytic microflora of apple surface without altering the growth of the targeted strains Ach 1-1 and 1113-5. The combination of the semi-selective medium and SCAR markers provides a valuable monitoring tool to specifically identify and quantify A. pullulans strain Ach 1-1 and strain 1113-5 and could be used in future studies to evaluate their population dynamics under various laboratory and practical conditions.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Development of SCAR markers and a semi-selective medium for the quantification of strains Ach 1-1 and 1113-5, two Aureobasidium pullulans potential biocontrol agents

Hamouchi

Bajji

Friel

et al. 2008

Postharvest Biology and Technology

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“…In general, liquid-phase cultures are used for bacteria and yeasts and solid-phase cultures are used for most fungi. Optimized mass production systems have been described for some postharvest biocontrol agents, including bacteria such as P. agglomerans CPA-2 (Costa et al, 2001), P. agglomerans PBC-1 (Manso et al, 2010) and B. subtilis CPA-8 (Yánez-Mendizábal et al, 2012b), yeasts such as C. sake CPA-1 (Abadias et al, 2003a,b), A. pullulans (Mounir et al, 2007), and Rhodotorula minuta (Patiño-Vera et al, 2005), and fungi such as Penicillium frequentans 909 (De Cal et al, 2002), and Epicoccum nigrum (Larena et al, 2004).…”

Section: Mass Production and Fermentationmentioning

confidence: 99%

“…Fluidized bed-drying is a costeffective method of drying that can be used to dry heat-sensitive microorganisms because the drying temperatures are relatively low. Fungi such as E. nigrum (Larena et al, 2003) and P. frequentans (Guijarro et al, 2006), the yeast-like fungus, A. pullulans (Mounir et al, 2007), and the yeast, C. sake CPA-2 (Usall et al, 2009) have all been successfully dried using fluidized bed-drying. In contrast, liquid formulations are the simplest way to stabilize the viability of microbial cells.…”

Section: Formulationmentioning

confidence: 99%

The science, development, and commercialization of postharvest biocontrol products

Droby

Wisniewski

Teixidó

et al. 2016

Postharvest Biology and Technology

287

149

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A B S T R A C TPostharvest biological control agents as a viable alternative to the use of synthetic chemicals have been the focus of considerable research for the last 30 years by many scientists and several commercial companies worldwide. Several antagonists of postharvest pathogens have been identified and tested in laboratory, semi-commercial, and commercial settings and were developed into commercial products. The discovery and development of these antagonists into a product followed a paradigm in which a single antagonist isolated from one commodity was also expected to be effective on other commodities that vary in their genetic background, physiology, postharvest handling, and susceptibility to pathogens. In most cases, product development was successfully achieved but their full commercial potential was not realized. The low success rate of postharvest biocontrol products has been attributed to several problems, including difficulties in mass production and formulation of the antagonist, the physiological status of the harvested commodity and its susceptibility to specific pathogens. All these factors played a major role in the reduced and inconsistent performance of the biocontrol product when used under commercial conditions. Although many studies have been conducted on the mode of action of postharvest microbial antagonists, our understanding is still very incomplete. In this regard, a systems approach, that takes into account all the components of the biocontrol system, may represent the best approach to investigating the network of interactions that exist. Very little is known about the overall diversity and composition of microbial communities on harvested produce and how these communities vary across produce types, their function, the factors that influence the composition of the microbiota after harvest and during storage, and the distribution of individual taxa. In light of the progress made in recent years in metagenomic technologies, this technology should be used to characterize the composition of microbial communities on fruit and vegetables. Information on the dynamics and diversity of microbiota may be useful to developing a new paradigm in postharvest biocontrol that is based on constructing synthetic microbial communities that provide superior control of pathogens.

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“…Vuill and Aspergillus niger Tiegh on table grapes (Castoria et al 2001), and the ochratoxigenic Aspergillus carbonarius (Bainier) Thom. on table grapes and strawberry (Lima et al 1997;Castoria et al 2001;De Felice et al 2008;De Curtis et al 2012b), against B. cinerea and P. expansum on apples (Castoria et al 2001;Ippolito et al 2000;Mounir et al 2007), against three species of the genus Monilinia on stone fruit (Mari et al 2012). De Felice et al (2008) observed for the first time that three strains of A. pullulans were the effective biocontrol agents in reducing both severity of Aspergillus rots in the vineyard and ochratoxin A (OTA) accumulation in wine grapes.…”

Section: Introductionmentioning

confidence: 99%

Antagonistic interactions between Aureobasidium pullulans and Fusarium culmorum, a fungal pathogen of winter wheat

Wachowska

Głowacka

2014

BioControl

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The effect of the biological control agent Aureobasidium pullulans (de Bary) G. Arnaud on the development of Fusarium head blight (FHB) on winter wheat and kernel contamination with fungi of the genera Fusarium, Acremonium, Cladosporium and Penicillium was analyzed in a greenhouse experiment. Scanning electron microscopy was used to evaluate the distribution of A. pullulans cells and aggregates on wheat kernels, infection structures of Fusarium culmorum (W.G. Smith) Sacc and the antagonist-pathogen interactions. Biological control with A. pullulans reduced FHB severity by 21.67 % and improved grain filling by 5.02 %, compared with the control treatment. The survival of A. pullulans was good (to 31 cells per kernel), in particular on the surface and in the crease of kernels, including in pathogen-inoculated wheat plants. A. pullulans cells firmly adhered to F. culmorum hyphae, and damaged them. In most cases, autochthonous communities of filamentous fungi of the genera Acremonium and Penicillium developed at a slower rate after kernel inoculation with the pathogen.

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Production, formulation and antagonistic activity of the biocontrol like-yeast Aureobasidium pullulans against Penicillium expansum

Cited by 33 publications

References 10 publications

Development of SCAR markers and a semi-selective medium for the quantification of strains Ach 1-1 and 1113-5, two Aureobasidium pullulans potential biocontrol agents

Development of SCAR markers and a semi-selective medium for the quantification of strains Ach 1-1 and 1113-5, two Aureobasidium pullulans potential biocontrol agents

The science, development, and commercialization of postharvest biocontrol products

Antagonistic interactions between Aureobasidium pullulans and Fusarium culmorum, a fungal pathogen of winter wheat

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