Effectiveness of antioxidant and anticoligenase activity of 70% ethanolic extracts of Kemangi leaves (Ocimum Basilicum)

Abstract

Free radicals are one form of reactive oxygen compounds, which are generally known as compounds that have unpaired electrons. Kemangi leaves are used as an aphrodisiac because they contain araginin which can strengthen sperm resistance and prevent infertility. Besides araginin, Kemangi leaves also contain other secondary metabolites such as essential oils, phytosterols, alkaloids, phenolic compounds, tannins, lignin, saponins, flavonoids, terpenoids and anthraquinones. Phytochemical screening results of 70% ethanol extract of kemangi herbs themselves showed the presence of secondary metabolites of flavonoids, saponins, tannins and triterpenoids / steroids. In this study, antioxidant activity was tested using the FRAP (Ferric Reducing Antioxidant Power) method, with concentrations of kemangi leaf extract and comparative compounds Euginol each concentration of 1000 μL/ml, 5000 μL/ml, 250 μL/ml, and 125 μL/ml, 62.50 μL/ml, and 31.25 μL/ml measured at 745 nm wavelength and collagenase inhibition test with ethanol extract of kemangi leaves with a comparison of eugenol compounds measured at a wavelength of 335 nm. The FRAP antioxidant activity results obtained based on IC50 Eugenol value of 261.36 μg/ml and Kemangi leaf extract at 111.32 μg/ml, antolagenagenase from Eugenol at 255.32 μg/ml and Kemangi leaf extract at 110.65 μ/ml.

The Potential of Tamarillo (Solanum betaceum) Peel as an Antioxidant by Radical Scavenging DPPH and Antiaging by Collagenase Inhibition

“…The macerates were concentrated and evaporated using a rotavapor at 50°C to obtain a paste form. The yield of S. betaceum peel extract (SBPE) was 5.45 g, stored at -20°C (Girsang et al, 2019;Priyandoko et al, 2022).…”

Hyaluronidase Inhibitory Activity and In Silico Docking Study of New Eugenol 1,2,3‐triazole Derivatives

“…[9,10] Previous researches reported that eugenol exhibit an inhibitory activity towards hyaluronidase enzyme. [11] In recent years, hyaluronidases inhibitors received extensive attention as a potent regulator that maintain hyaluronic acid homeostasis and they might serve as anti-aging, anticancer, anti-inflammatory, antimicrobial as well as anti-venom and contraceptive agents. [12] Based on hybridization approach, we aimed to investigate the incorporation of different bioactive moieties with different chemical properties and variable connecting linkers into our main eugenol skeleton hoping to obtain compounds with synergistic hyaluronidase inhibitory activity.…”