Effective PCR Systems for Rapid Identification of Human Papillomaviruses (HPVS)

Identification of HPV DNA in patients with juvenile-onset recurrent respiratory papillomatosis using SYBR® Green real-time PCR

“…The cells from pharyngeal swabs of healthy patients were used as control samples. Both biopsies and cells were collected in sterile jars containing transport medium [8] and delivered for a study. Approval for the study was obtained from the Independent Ethics Committee of MBAL ''Queen Ioanna''.…”

“…The quality of sample DNA was validated by amplification of a 110 bp fragment of the housekeeping gene b-globin, as described previously [8]. Aliquots (10 ml) of each PCR product were resolved by electrophoresis in a 2% agarose gel and stained with ethidium bromide.…”

Quantitative Analysis of High-Risk Human Papillomavirus Types in Abnormal Cervical Smears

“…Twenty women with known abnormal cytological cervices (Pap smear grade 3-d) were included in the study. Samples of cervical cells taken by cytobrush were obtained from each woman and were resuspended in transport buffer (7). Cells were washed twice and resuspended in 200 µl TE buffer (pH 7.2).…”

“…This primer pair is routinely used in our lab. To control internally the quality of the isolated DNA, the 110 bp sequence of β-globin gene was co-amlified using PC03 and PC04 primers in the multiplex PCR with the MY primers (7). After the consensus primer-based PCR, the PCR using primers specific for HPV types 6, 11, 16, 18, 31, 33 was performed using respective amplification protocol and conditions described previously (7).…”

Identification of Multiple Human Papillomavirus (HPV) Genotypes in a Patient with Oral Condylomata Acuminata

“…Total cellular DNA was extracted by DNAzol (Invitrogen, USA) and portions of 1-2 μl (0.5-1.0 μg) were analyzed by PCR, using the protocols described previously (6,7). In order to detect the presence of HPV DNA, the samples were initially tested by a set of consensus primers MY 09/11 (8).…”