Identification of HPV DNA in patients with juvenile-onset recurrent respiratory papillomatosis using SYBR® Green real-time PCR

Draganov, Peter V.; Todorov, Sp.; Todorov, Ivan; Karchev, Todor; Kalvatchev, Z.

doi:10.1016/j.ijporl.2005.07.018

Cited by 54 publications

(55 citation statements)

References 14 publications

(19 reference statements)

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“…Although this suggests that typing may be a useful prognostic marker, more patients will need to be investigated to confirm if HPV-11 is indeed associated with a more aggressive disease as this was a small series of patients. Most other studies have found HPV-11 disease to be more aggressive [2][3][4][5][13][14][15][16]. Recent studies have highlighted Ki-67 expression and E6 and E7 oncogene expression as potential predictors of disease aggressiveness and this also requires further investigation [16,26].…”

Section: Discussionmentioning

confidence: 98%

HPV types causing juvenile recurrent laryngeal papillomatosis in South Africa

Seedat

Thukane

Jansen

et al. 2010

International Journal of Pediatric Otorhinolaryngology

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Section: Discussionmentioning

confidence: 98%

HPV types causing juvenile recurrent laryngeal papillomatosis in South Africa

Seedat

Thukane

Jansen

et al. 2010

International Journal of Pediatric Otorhinolaryngology

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“…By the use of highly sensitive SYBR-green real-time PCR, HPV has been found in 100% of papillomas from RRP patients [20]. It is clear that the sensitivity of technique used for HPV detection is relevant although it is also important to analyse the viral sub type, plus any variants that may be present.…”

Section: Viral Aetiologymentioning

confidence: 99%

The role of HPV type in Recurrent Respiratory Papillomatosis

Donne

Hampson

Homer

et al. 2010

International Journal of Pediatric Otorhinolaryngology

122

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“…Finally, the HPV-16 and HPV-11 E7ER recapitulated the induction of p21cip1 protein in differentiated cells negative for BrdU incorporation when BrdU was added to the medium for 6 to 12 h immediately prior to harvest, as previously observed in patient specimens infected by the low-risk HPVs and in raft cultures transduced with pLC-18URR18E7. It is also clinically relevant to note that the higher efficiency of HPV-11 E7ER in inducing S-phase reentry in differentiated cells relative to HPV-6 E7ER might potentially explain the more aggressive regrowth of laryngeal papillomas caused by HPV-11 relative to those caused by HPV-6 after surgical excision (10,30,39).…”

Section: Vol 80 2006 Low-risk Hpv E7 Induces S Phase In Differentiamentioning

confidence: 99%

Conditionally Activated E7 Proteins of High-Risk and Low-Risk Human Papillomaviruses Induce S Phase in Postmitotic, Differentiated Human Keratinocytes

Banerjee

Genovese

Noya

et al. 2006

J Virol

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The productive program of human papillomaviruses (HPVs) in epithelia is tightly linked to squamous differentiation. The E7 proteins of high-risk HPV genotypes efficiently inactivate the pRB family of proteins that control the cell cycle, triggering S phase in suprabasal keratinocytes. This ability has until now not been demonstrated for the low-risk HPV-6 or HPV-11 E7 proteins. An inducible system in which HPV-16 E7 is fused to the ligand binding domain of the human estrogen receptor (ER) was described by In the absence of hormone, E7ER is cytoplasmic, and upon addition of 17␤-estradiol, it translocates to the nucleus. Using organotypic epithelial raft cultures developed from primary human keratinocytes, we show that 16E7ER promotes either S-phase reentry or p21cip1 accumulation in differentiated keratinocytes in a stochastic manner as early as 6 h postinduction with 17␤-estradiol. A vector expressing the ER moiety alone had no effect. These observations prove unequivocally that the E7 protein drives S-phase reentry in postmitotic, differentiated keratinocytes rather than preventing S-phase exit while the cells ascend through the epithelium. HPV-11 E7ER and, much less efficiently, HPV-6 E7ER also promoted S-phase reentry by differentiated cells upon exposure to 17␤-estradiol. S-phase induction required the consensus pRB binding motif. We propose that the elevated nuclear levels of the low-risk HPV E7 protein afforded by the inducible system account for the positive results. These observations are entirely consistent with the fact that low-risk HPV genotypes replicate in the differentiated strata in patient specimens, as do the high-risk HPVs.Human papillomaviruses (HPVs) are small DNA viruses that infect cutaneous or mucosal epithelia. The closely related HPV type 6 (HPV-6) and HPV-11 are low-risk (LR) genotypes and cause benign warts in the anogenital tract and laryngeal papillomas in the upper respiratory mucosal epithelium; these infections rarely progress to cancers. In contrast, HPV-16, HPV-18, and related types are high-risk (HR) viruses, and their infections can lead to high-grade squamous intraepithelial neoplasias that pose a risk of further progression to carcinomas in situ and invasive carcinomas (47). HPV infection is thought to initiate in the undifferentiated basal stratum, presumably gaining access through a wound. The double-stranded circular DNA genome is maintained in the undifferentiated basal stratum as extrachromosomal nuclear plasmids at approximately 20 to 50 copies per cell. Viral-DNA amplification and progeny virion production are only observed in the more superficial, differentiated strata of the squamous epithelium (9, 22, 36; see reference 6 for a review). Because viral-DNA replication depends on the host DNA replication machinery to complement the viral origin binding protein E2 and the viral DNA helicase E1 (7), the virus must reestablish an S-phase milieu in the differentiated strata to achieve productive infections.The high-risk HPV E7 proteins efficiently target the key tumor sup...

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Identification of HPV DNA in patients with juvenile-onset recurrent respiratory papillomatosis using SYBR® Green real-time PCR

Cited by 54 publications

References 14 publications

HPV types causing juvenile recurrent laryngeal papillomatosis in South Africa

HPV types causing juvenile recurrent laryngeal papillomatosis in South Africa

The role of HPV type in Recurrent Respiratory Papillomatosis

Conditionally Activated E7 Proteins of High-Risk and Low-Risk Human Papillomaviruses Induce S Phase in Postmitotic, Differentiated Human Keratinocytes

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