Effect of zinc oxide addition on antimicrobial and antibiofilm activity of hydroxyapatite: a potential nanocomposite for biomedical applications

Beyene, Zerihun; Ghosh, Rupita

doi:10.1016/j.mtcomm.2019.100612

Cited by 41 publications

(25 citation statements)

References 31 publications

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“…Studies have shown that ZnO with small size is an ideal non-polar antibacterial agent with broad-spectrum antibacterial effects [ 15 , 16 ]. Based on several reports, the ZnO/HA composites, prepared by using ZnO as a second phase or HA being modified with ZnO, still had broad-spectrum antibacterial properties [ 17 ]. However, a series of problems have been found during the preparation and clinical application of HA composites, such as the uncontrollable grain growth for HA and ZnO during sintering, low or no antimicrobial properties, and unsuitable mechanical properties for human bone tissue, which limit the application of HA composites.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Microstructure of ZnO/HA Composite with In Situ Formation of Second-Phase ZnO

Yuan

et al. 2020

Materials

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Nanometer hydroxyapatite (n-HA) powders were synthesized by the chemical precipitation method, and a novel ZnO/HA composite, which consisted of second-phase particles with different sizes and distributions, was successfully fabricated. ZnO/HA composites were prepared by using powder sintering with different Zn contents and a prefabrication pressure of 150 MPa. Microstructure and local chemical composition were analyzed by a scanning electron microscope (SEM) and energy-dispersive spectrometer (EDS), respectively. The phase composition and distribution of the composite were determined with electron back-scattered diffraction (EBSD) and an X-ray diffractometer (XRD), respectively. The experimental results of the XRD showed that the chemical precipitation method was a simple and efficient method to obtain high-purity n-HA powders. When the sintering temperature was lower than 1250 °C, the thermal stability of HA was not affected by the Zn in the sintering process. Due to sintering in an air atmosphere, the oxidation reaction of Zn took place in three stages, and ZnO as the second phase had two different sizes and distributions in the composites. The compressive strength of ZnO/HA composites, of which the highest was up to 332 MPa when the Zn content was 20%, was significantly improved compared with pure HA. The improvement in mechanical properties was mainly due to the distribution of fine ZnO particles among HA grains, which hindered the HA grain boundary migration and refinement of HA grains. As grain refinement increased the area of the grain boundary inside the material, both the grain boundary and second phase hindered crack development in different ways.

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Section: Introductionmentioning

confidence: 99%

Fabrication and Microstructure of ZnO/HA Composite with In Situ Formation of Second-Phase ZnO

Yuan

et al. 2020

Materials

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“…On the nanoscale, ZnO shows exceptional physicochemical properties that are beneficial in many industrial applications. Many researchers have successfully used ZnO nanostructures in photocatalytic [13][14][15][16], photovoltaic [17][18][19][20], biomedical [21][22][23][24] and sensing [25][26][27][28][29][30][31] applications, as it provides large surface area as compared to its bulk counterpart. The demand of highly efficient, durable and robust photocatalysts for wastewater treatment makes ZnO nanostructures a reliable candidate for photocatalytic applications.…”

Section: Introductionmentioning

confidence: 99%

One-Pot Sonochemical Synthesis of ZnO Nanoparticles for Photocatalytic Applications, Modelling and Optimization

et al. 2019

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This present study proposed a successful one pot synthesis of zinc oxide nanoparticles (ZnO NPs) and their optimisation for photocatalytic applications. Zinc chloride (ZnCl2) and sodium hydroxide (NaOH) were selected as chemical reagents for the proposed study. The design of this experiment was based on the reagents’ amounts and the ultrasonic irradiations’ time. The results regarding scanning electron microscopy (SEM), X-ray diffraction (XRD) and Raman spectroscopy confirmed the presence of ZnO NPs with pure hexagonal wurtzite crystalline structure in all synthesised samples. Photocatalytic activity of the developed samples was evaluated against methylene blue dye solution. The rapid removal of methylene blue dye indicated the higher photocatalytic activity of the developed samples than untreated samples. Moreover, central composite design was utilised for statistical analysis regarding the obtained results. A mathematical model for the optimisation of input conditions was designed to predict the results at any given point. The role of crystallisation on the photocatalytic performance of developed samples was discussed in detail in this novel study.

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“…Additionally, the growth percentage was calculated using Equation (1). Death kinetic curves were also plotted, by controlling the Candida cells through estimated IC50 values of Griseofulvin and prepared Ge-Nco [ 18 ].

…”

Section: Methodsmentioning

confidence: 99%

Enhancing the Antifungal Activity of Griseofulvin by Incorporation a Green Biopolymer-Based Nanocomposite

et al. 2021

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Fungal biofilms have caused several medical problems, resulting in significant morbidity and mortality as well as poor response to antifungal drugs. The current study was designed to evaluate the enhancement of antifungal and anti-biofilm activity of Griseofulvin-loaded green nanocomposite-based biopolymers (Ge-Nco) of glycogen and gelatin against different strains of pathogenic Candida species. The prepared Ge-Nco was characterized using Fourier-transform infrared (FT-IR), X-ray diffraction pattern (XRD), scanning electron microscopy-energy dispersive X-ray (SEM-EDX) and transmission electron microscope (TEM). In addition, the morphology of the mature biofilm and the inhibition of biofilm was monitored and visualized using confocal laser scanning microscopy (CLSM). The minimal inhibitory concentrations (MIC) and (IC50) of Griseofulvin alone and the prepared Ge-Nco against three different strains of Candida sp. were determined according to Clinical and Laboratory Standards Institute (CLSI) method. The effects of Griseofulvin alone and Ge-Nco on the tested Candida sp. biofilm formation were determined by the crystal-violet staining protocol. The biofilm inhibition potential of Ge-Nco against the tested Candida sp. was detected and depicted under CLSM (2.5 D view). The findings depicted that Ge-Nco was prepared in nanometer size (10–23 nm). The observed minimum inhibitory concentration (MIC) of Griseofulvin alone and Ge-Nco against three different Candida sp. were found to be in range 49.9–99.8 μg/mL and 6.24–12.48 μg/mL, respectively. These results provide evidence for implementing efficient antivirulence approaches against three different Candida sp. that would be less likely to foster the emergence of resistance.

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Effect of zinc oxide addition on antimicrobial and antibiofilm activity of hydroxyapatite: a potential nanocomposite for biomedical applications

Cited by 41 publications

References 31 publications

Fabrication and Microstructure of ZnO/HA Composite with In Situ Formation of Second-Phase ZnO

Fabrication and Microstructure of ZnO/HA Composite with In Situ Formation of Second-Phase ZnO

One-Pot Sonochemical Synthesis of ZnO Nanoparticles for Photocatalytic Applications, Modelling and Optimization

Enhancing the Antifungal Activity of Griseofulvin by Incorporation a Green Biopolymer-Based Nanocomposite

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