Effect of zinc-doped hydroxyapatite/graphene nanocomposite on the physicochemical properties and osteogenesis differentiation of 3D-printed polycaprolactone scaffolds for bone tissue engineering

Maleki-Ghaleh, H.; Siadati, M. H.; Fallah, Ali; Zarrabi, Ali; Afghah, Ferdows; Koç, Bahattin; Abdolahinia, Elaheh Dalir; Omidi, Yadollah; Barar, Jaleh; Akbari‐Fakhrabadi, Ali; Beygi‐Khosrowshahi, Younes; Adibkia, Khosro

doi:10.1016/j.cej.2021.131321

Cited by 67 publications

(28 citation statements)

References 83 publications

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“…The X-ray photoelectron spectroscopy (XPS), XRD, and attenuated total reflectance-Fourier transform infrared (ATR-FTIR) characterizations of the nanoparticles have been thoroughly studied in the previous work [34]. The TEM images for (a) HA, (b) ZnHA, and (c) ZnHA-rGO nanoparticles are presented in Figure 1.…”

Section: Characterization Of Nanoparticlesmentioning

confidence: 99%

“…The difference in ionic radius between Zn 2+ and Ca 2+ can distort the PO4 3− structure [40,41]. Moreover, the interaction between the rGO atoms with the surface atoms of HA nanoparticles can affect the structure of PO4 3− [34]. Figure 3a shows the Raman spectra for HA, ZnHA, and ZnHA-rGO nanoparticles.…”

Section: Characterization Of Nanoparticlesmentioning

confidence: 99%

“…Extraction of natural HA nanoparticles was performed according to the method presented in a previous study [34]. Doping Zn in HA nanoparticles and compositing it with rGO has also been described in a previous study [34].…”

Section: Materials Preparationsmentioning

confidence: 99%

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Antibacterial and Cellular Behaviors of Novel Zinc-Doped Hydroxyapatite/Graphene Nanocomposite for Bone Tissue Engineering

Maleki-Ghaleh

Siadati

Fallah

et al. 2021

IJMS

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Bacteria are one of the significant causes of infection in the body after scaffold implantation. Effective use of nanotechnology to overcome this problem is an exciting and practical solution. Nanoparticles can cause bacterial degradation by the electrostatic interaction with receptors and cell walls. Simultaneously, the incorporation of antibacterial materials such as zinc and graphene in nanoparticles can further enhance bacterial degradation. In the present study, zinc-doped hydroxyapatite/graphene was synthesized and characterized as a nanocomposite material possessing both antibacterial and bioactive properties for bone tissue engineering. After synthesizing the zinc-doped hydroxyapatite nanoparticles using a mechanochemical process, they were composited with reduced graphene oxide. The nanoparticles and nanocomposite samples were extensively investigated by transmission electron microscopy, X-ray diffraction, and Raman spectroscopy. Their antibacterial behaviors against Escherichia coli and Staphylococcus aureus were studied. The antibacterial properties of hydroxyapatite nanoparticles were found to be improved more than 2.7 and 3.4 times after zinc doping and further compositing with graphene, respectively. In vitro cell assessment was investigated by a cell viability test and alkaline phosphatase activity using mesenchymal stem cells, and the results showed that hydroxyapatite nanoparticles in the culture medium, in addition to non-toxicity, led to enhanced proliferation of bone marrow stem cells. Furthermore, zinc doping in combination with graphene significantly increased alkaline phosphatase activity and proliferation of mesenchymal stem cells. The antibacterial activity along with cell biocompatibility/bioactivity of zinc-doped hydroxyapatite/graphene nanocomposite are the highly desirable and suitable biological properties for bone tissue engineering successfully achieved in this work.

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Section: Characterization Of Nanoparticlesmentioning

confidence: 99%

Section: Characterization Of Nanoparticlesmentioning

confidence: 99%

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Antibacterial and Cellular Behaviors of Novel Zinc-Doped Hydroxyapatite/Graphene Nanocomposite for Bone Tissue Engineering

Maleki-Ghaleh

Siadati

Fallah

et al. 2021

IJMS

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“…In case of the projection onto (100) plane, there are possibly at least two cross-sections shown in Figure A5 e,g ( Appendix A ). According to [ 41 ], the apatite structure can be considered as build of layers parallel to ac showing the sequence –B–A-A–B– along [ 10 ], where A = Ca 3 (PO 4 ) 2 and B = Ca 4 (PO 4 ) 2 (F,OH) 2 (compare Figure A5 f,h, Appendix A ). In Figure A5 e ( Appendix A ), the layers A-A (PO 4 -rich), shown in projection onto (100), are composed of parallel and alternating strips of Ca 2+ and PO 4 3− ions.…”

Section: Resultsmentioning

confidence: 99%

“…Due to its structural similarity to biological bone, visible biocompatibility, and bioactivity, HAp has been applied in medicine and dentistry in several forms: as dense sintered ceramics; porous forms; granules; and coatings of metal implants [ 8 , 9 , 10 ]. The shape of HAp crystallites is highly anisotropic, with at least two types of crystal faces: (100) and (101), and rarely (001), exhibiting different distributions of electrostatic potential, different interactions with water, and different adsorptions of biomolecules [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Nature-Inspired Effects of Naturally Occurring Trace Element-Doped Hydroxyapatite Combined with Surface Interactions of Mineral-Apatite Single Crystals on Human Fibroblast Behavior

Tyszka-Czochara

Suder

Dołhańczuk-Śródka

et al. 2022

IJMS

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Innovative engineering design for biologically active hydroxyapatites requires enhancing both mechanical and physical properties, along with biocompatibility, by doping with appropriate chemical elements. Herein, the purpose of this investigation was to evaluate and elucidate the model of naturally occurring hydroxyapatite and the effects of doped trace elements on the function of normal human fibroblasts, representing the main cells of connective tissues. The substrates applied (geological apatites with hexagonal prismatic crystal habit originated from Slyudyanka, Lake Baikal, Russia (GAp) and from Imilchil, The Atlas Mountains, Morocco (YAp)) were prepared from mineral natural apatite with a chemical composition consistent with the building blocks of enamel and enriched with a significant F− content. Materials in the form of powders, extracts and single-crystal plates have been investigated. Moreover, the effects on the function of fibroblasts cultured on the analyzed surfaces in the form of changes in metabolic activity, proliferation and cell morphology were evaluated. Apatite plates were also evaluated for cytotoxicity and immune cell activation capacity. The results suggest that a moderate amount of F− has a positive effect on cell proliferation, whereas an inhibitory effect was attributed to the Cl− concentration. It was found that for (100) GAp plate, fibroblast proliferation was significantly increased, whereas for (001) YAp plate, it was significantly reduced, with no cytotoxic effect and no immune response from macrophages exposed to these materials. The study of the interaction of fibroblasts with apatite crystal surfaces provides a characterization relevant to medical applications and may contribute to the design of biomaterials suitable for medical applications and the evaluation of their bioavailability.

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Additive Manufacturing Techniques for Designing Advanced Scaffolds for Bone Tissue Engineering

Pottathara¹,

Kokol²

2022

Nanotechnology‐Based Additive Manufacturing

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Effect of zinc-doped hydroxyapatite/graphene nanocomposite on the physicochemical properties and osteogenesis differentiation of 3D-printed polycaprolactone scaffolds for bone tissue engineering

Cited by 67 publications

References 83 publications

Antibacterial and Cellular Behaviors of Novel Zinc-Doped Hydroxyapatite/Graphene Nanocomposite for Bone Tissue Engineering

Antibacterial and Cellular Behaviors of Novel Zinc-Doped Hydroxyapatite/Graphene Nanocomposite for Bone Tissue Engineering

Nature-Inspired Effects of Naturally Occurring Trace Element-Doped Hydroxyapatite Combined with Surface Interactions of Mineral-Apatite Single Crystals on Human Fibroblast Behavior

Additive Manufacturing Techniques for Designing Advanced Scaffolds for Bone Tissue Engineering

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