Effect of wilting, silage additive, PEG treatment and tannin content on the distribution of N between different fractions after ensiling of three different sainfoin (Onobrychis viciifolia) varieties

Abstract

This is an author produced pre-print version of a paper later published in Grass and Forage Science.Citation for the published paper: Lorenz, M.M., Eriksson, T., Udén, P. additive. Addition of PEG to the silage increased the BSN-proportion 1.8-and 2.6-fold for both 39 DM stages. A strong tannin-protein binding effect is, therefore, confirmed in sainfoin. However, 40 correlations between tannin levels (ET and PBT) and BSN were poor in the (non-PEG) silages, 41 indicating either that the HCl/butanol method is unsuitable for measuring tannin in silages or that 42 qualitative attributes of tannins are more relevant than quantitative. The HCl/butanol method 43 seems therefore not to be useful to predict degradation of protein in sainfoin silages. 44 45

The effect of bale density and addition of formic acid on the <i>in situ</i> dry matter and crude protein degradation of lucerne, red clover and red fescue silages

“…The inhibition of proteolysis may be reflected in reduced rumen protein degradability in acidified legume or grass silages. Pursiainen and Tuori (2008) and Lorenz et al (2010) demonstrated decreased protein degradation in preserved silages. In contrast, Siddons et al (1982) showed no effect or only slightly reduced degradability of protein in FA-treated ryegrass or red clover silages; more effective than FA alone was a mixture of FA and formaldehyde.…”

Influence of formic acid and dry matter on protein degradation in the tanniniferous legume sainfoin

“…Studies by 57 Pursiainen and Tuori (2008) and Lorenz (2010) showed decreased protein degradation in acidified legume 58 silages. 59…”

Quantitative Traits Breeding for Multifunctional Grasslands and Turf

“…Polyphenols, including tannins, are often analysed by relatively non-specifi c colorimetric methods, which provide little new information (Lorenz et al 2010 ). LC-MS methods are capable of yielding much more useful information and one such method was specifi cally developed for screening fl avonoids, cinnamic acid derivatives and other polyphenols in this sainfoin germplasm (Regos et al 2009 ).…”

“…Sainfoin is the only perennial forage legume that contains a high watersoluble carbohydrate content; i.e. They are also responsible for the fact that sainfoin can be relatively easily ensiled in contrast to most other forage legumes (Lorenz et al 2010 ). Sucrose was a major metabolite and accounted for approximately 35 % of the acetone/water extract (Marais et al 2000 ).…”

“…In addition harvesting and preservation procedures will need to be optimised in order to maintain the benefi cial effects of sainfoin and other bioactive forage legumes (Lorenz et al 2010 ). In addition harvesting and preservation procedures will need to be optimised in order to maintain the benefi cial effects of sainfoin and other bioactive forage legumes (Lorenz et al 2010 ).…”