Influence of formic acid and dry matter on protein degradation in the tanniniferous legume sainfoin

Abstract

This is an author produced pre-print version of a paper later published in Animal Feed Science and Technology.Citation for the published paper: Lorenz, M.M., Udén, P. (2011) Criteria for tannin binding effects were based on N fractionation in an in vitro ruminal digestion system. A 28 mixture of sainfoin varieties were wilted to different dry matter (DM) levels and treated with 0, 4 and 8 ml 29 formic acid per kg fresh matter prior to ensiling for 60 days. Nitrogen fractionation included measurement 30 of total N, buffer soluble N, non-protein N, α-amino acid N and ammonia N. Protein degradation was 31 measured by an in vitro ruminal protein digestion system. Results show beneficial effects of moderate and 32 high acidification particularly for buffer soluble N at 180 and 400 g/kg DM (p<0.0001) while these effects 33 level off at 500 and 600 g/kg DM (P<0.852). However, no negative effect related to impaired tannin 34 protein binding in the silages or the in vitro protein degradation occurred. 35

Sodium Hydroxide Enhances Extractability and Analysis of Proanthocyanidins in Ensiled Sainfoin (Onobrychis viciifolia)

“…Ensiling appears to affect mainly extractable proanthocyanidins, which accounted for 81% in the fresh but for only 18% in the ensiled samples. This implies that ensiling substantially increased the proportion of residual or bound proanthocyanidins (Table ) and is in accord with literature data. ,, It would appear that NaOH affected the measured proanthocyanidin content by releasing bound proanthocyanidins from the residue (Tables and ).…”

“…This implies that ensiling substantially increased the proportion of residual or bound proanthocyanidins (Table 1) and is in accord with literature data. 7,8,21 It would appear that NaOH affected the measured proanthocyanidin content by releasing bound proanthocyanidins from the residue (Tables 1 and 3).…”

Nitrogen metabolism and milk production in dairy cows fed semi‐restricted amounts of ryegrass–legume silage with birdsfoot trefoil (Lotus corniculatus L.) or white clover (Trifolium repens L.)

“…() found a protein‐bound tannin proportion of 0·70 in sainfoin silage, twice that in the BFT silage fed in our experiment, with identical analytical methodology. The pH range of 4·5–5·0 in the BFT silage most likely promoted the persistence of tannin–protein complexes (Jones and Mangan, ; Lorenz and Udén, ), so the low protein‐bound proportion may be caused by failure to create bonds rather than the dissociation of complexes.…”