Effect of Wild Lactobacillus buchneri Strains on the Fermentation Profile and Microbial Populations of Sugarcane Silage

Abstract

Background: Sugarcane silage has been increasing as a feed in the tropics by dairy farmers. However, sugarcane normally had high yeast population that leads to intense alcoholic fermentation and excessive Dry-Matter (DM) loss during ensilage and after air exposure, as well. There are several patents that have recently shown the benefits of applying Lactobacillus buchneri in forage preservation. Objective: This study aimed to investigate the changes in pH, DM, Water-Soluble Carbohydrates (WSC) and fermentation end product concentrations that occur in sugarcane silage with or without inoculation with L. buchneri after 45 days of ensiling. Methods: Sugarcane plants were harvested with approximately 16 months of growth and chopped at 2 cm. Four strains of wild L. buchneri (56.1, 56.4, 56.9 and 56.26) and the commercial inoculant “Lalsil Cana” were evaluated. For all treatments, the theoretical application rate was 1.0 × 106 colony- forming units (cfu) per g of fresh weight. Data from the silo openings were analysed as a completely randomized design, with four replicates per treatment (inoculants). Results: The treatment with L. buchneri affected the DM content, pH, Lactic Acid Bacteria (LAB) population, DM recovery, and concentrations of WSC, lactic acid, acetic acid and ethanol of sugarcane silage after 45 days of ensiling. Yeasts and molds populations and the concentrations of propionic and butyric acids were not affected by the treatments. Conclusion: Lactobacillus buchneri 56.1 and 56.4 are considered the most suitable strains for improving the fermentation of sugarcane silage and thus are potential inoculants for silage production. At present, we are preparing the patent application.

Antidiabetic Activity of Potential Probiotics Limosilactobacillus spp., Levilactobacillus spp., and Lacticaseibacillus spp. Isolated from Fermented Sugarcane Juice: A Comprehensive In Vitro and In Silico Study

“…Some studies emphasize the aid of Lactobacillus spp. for improving fermentation of the silage, bagasse, and profiling species obtained in the sugarcane juice and sugarcane processing steams [28][29][30]. Ellis et al claim that filtered sugarcane molasses concentrate, which is high in minerals and phytonutrients and is typically ingested with foods that include carbohydrates as a functional ingredient, has the capacity to reduce insulin responses and lessen the load on the pancreatic beta cells.…”

>b<Suplementação com culturas vivas de >i<Aspergillus oryzae>/i< e enzima fibrolítica para bovinos de leite:>/b< efeitos sobre o metabolismo, cinética e ecossistema ruminal

“…Entretanto, a utilização de aditivos na ensilagem da cana-de-açúcar vem se demonstrado uma estratégia eficiente no controle da proliferação das leveduras no silo. A bactéria homofermentativa L. buchneri vem apresentando resultados positivos sobre a qualidade da silagem de cana-de-açúcar (NUSSIO et al, 2003) e na silagem de milho , visto que o produto final da fermentação dos carboidratos solúveis é o acetato, que tem ação inibitória sobre as leveduras SILVA et al, 2019). Outra estratégia seria promover a liberação de carboidratos dos constituintes fibrosos afim de elevar a produção de lactato pelas bactérias ácido láticas, promovendo rápida acidificação da silagem, evitando assim a proliferação de microrganismos indesejáveis (MUCK et al, 2018…”