Effect of White Turmeric Extract (Curcuma zedoaria) Using Zam-zam Solvent Compare with Ethanol Solvent Against Breast Cancer Cell T47D

Abstract

<jats:p>Introduction: Curcuma zedoaria is one of the herbal plants potentially protects and cures many diseases, particularly as anti-cancer and anti-tumor. Anti-cancer active compounds of it are flavonoid, phenolic, and saponin. Objectives: This study aimed to explore the cytotoxicity of Curcuma zedoria extract (CZE). Methods: Experimental Quasi with post test non equivalent control group design on T47D cell line at Biology laboratory, Faculty of Medicine, Sultan Agung Islamic University, Semarang. The research was consisted of 2 groups, namely: intervention group with CZE zam-zam solvent and intervention group with CZE ethanol solvent given 10 different dosages each for 1.000 μg/mL; 500 μg/mL; 250 μg/mL; 125 μg/mL; 62.5 μg/mL; 31.25 μg/mL; 15.62 μg/mL; 7.81 μg/mL; 3.90 μg/mL; 1.95 μg/mL. Cytotoxicity test of IC50 using direct counting method and analyzed by probit analysis. Results: IC50 value of CZE in zam-zam and ethanol solvent were 28.24 μg/ml and 13.71 µg/ml respectively at the same 24 hours incubation period. Chi-square test revealed p value was 0.000 (α = 0.05), meaning that there was significant relationship. Conclusion: CZE activity using ethanol is highly active while CZE using zam-zam water is moderate and both of them have the toxicity on breast cancer cell. As the result, apoptosis process may occur.</jats:p>

Effect of Zamzam Water Extract of Curcuma zedoaria Treatment on Apoptosis, p53 and Bcl2 Protein Expression of T47D Cell Culture

“…According to phytochemical analysis, zamzam water extract of CZ contained flavonoids, phenol, and saponin and their concentration were lower compared to that of ethanolic extract of CZ 18 . The calculated IC 50 value of CZ zamzam water extract was 28.24 pg/ml or 20 μg/ml slightly higher compared to that of ethanol extract of CZ 13.71 pg/ml or less than 20 μg/ml.…”

“…Based on phytochemistry analysis, zamzam water extract of CZ contained higher flavonoids, phenolic, and saponin content compared to that of plain tap water 18 . However, the effect of zamzam water extract of CZ remains unclear.…”

Antioxidant and antibacterial activities of the rhizome extract of Curcuma zedoaria extracted using some organic solvents

“…At doses of 100, 250, 500, and 1000 mg/ml, the rhizome extract of C. zedoaria effectively inhibits the growth of Streptococcus mutants , Enterococcus faecalis, Staphylococcus aureus, and Candida albicans and could reduce as much as 99.99% of the population of microbes [ 12 ]. Another beneficial effect of this white turmeric is that it is not only used for the maintenance of physical health but is also advantageous for curing various diseases such as cancer or tumors [ 13 , 14 ]. Dosoky and Setzer [ 15 ] affirm that the extract of C. zedoaria rhizome shows anticancer characteristics, antiinflammatory, analgesic, antiallergic, antiparasitic against Entamoeba histolytica , antibacterial, and antifungal activity.…”

“…In previous studies, the extraction of phytochemicals from the C. zedoaria rhizome was conducted merely using ethanol or methanol (MeOH) [ 14 , 18 ]. Ethanol or MeOH solvents are polar solvents that can only extract polar compounds.…”