Effect of Zamzam Water Extract of Curcuma zedoaria Treatment on Apoptosis, p53 and Bcl2 Protein Expression of T47D Cell Culture

Abstract

Introduction:People worldwide drink Zamzam water either medicinally or religiously. However, the effect of zamzam water extract of CZ in breast cancer cells remains unclear. Objective: To evaluate the zamzam water extract of CZ effect on cell apoptosis, p53 and Bcl2 protein expression in T47 D cells line culture. Methods: In the control group design, 16 Wells which was filled with T47 D cell line culture in the density of 5 x 10 4 /100 µl, were assigned into 4 groups. Control group (C-G), were only filled with T47D cell line culture; Zamzam 50 (ZZ-50); Zamzam 100 (ZZ-100), and Zamzam 500 (ZZ-500) groups, were filled with T47D cell line cultures and 50, 100, and 500 µg/ml zamzam water extract of CZ respectively, and then incubated for 48 hours. The number of apoptotic cell was counted by flow cytometry, while p53 and Bcl2 measured by immunohistochemistry staining method. Results: Mann Whitney analysis indicated that the percentage of apoptotic cell and p53 protein expression in ZZ-100 was significant higher compared to that of C-G (p < 0.05). In contrary the percentage number of Bcl2 protein expression in ZZ100-G was significantly lower when compared to that of C-G (p < 0.05). Conclusion: Treatment of zamzam water extract of CZ on T47D cell line cultured was capable of increasing the number of apoptotic cells, p53 protein expression, but decreased Bcl2 protein expression in T47D cell line culture. However the numbers of apoptotic cells were less than 10%.

Both Ethanol and Ethyl Acetate Curcuma Zedoaraia Extract was Capable of Inducing Cells Death in T47D Cell Line Culture

“…6,7 My own study that have been published indicated that the potential effect of water extract of CZ on T47D cells apoptosis remains low, less than 10%. 6 On the other hand, some evidences showed that both ethanol and ethyl acetate CZ extract has been proven capable of inducing apoptosis on various cancer cells. [6][7][8] However, the potential effect of ethanol and ethyl acetate CZ extract which one is more effective on T47D cells apoptosis remain unclear.…”

“…3 The decrease in BC mortality rate was attributable to screening program, improvement of treatment, earlier detection, and adjuvant therapy originate from botanical product. 3,5,6 Curcuma zedoaria (CZ) is a botanical product has been proven capable of inducing apoptosis on various cells such as Daltons Lymphoma Ascites (DLAs), Lung Carcinoma (A549), nasopharyngeal carcinoma (KB), leukemic (K562), and T47D cell lines. 6,7 My own study that have been published indicated that the potential effect of water extract of CZ on T47D cells apoptosis remains low, less than 10%.…”

“…3,5,6 Curcuma zedoaria (CZ) is a botanical product has been proven capable of inducing apoptosis on various cells such as Daltons Lymphoma Ascites (DLAs), Lung Carcinoma (A549), nasopharyngeal carcinoma (KB), leukemic (K562), and T47D cell lines. 6,7 My own study that have been published indicated that the potential effect of water extract of CZ on T47D cells apoptosis remains low, less than 10%. 6 On the other hand, some evidences showed that both ethanol and ethyl acetate CZ extract has been proven capable of inducing apoptosis on various cancer cells.…”

“…6 On the other hand, some evidences showed that both ethanol and ethyl acetate CZ extract has been proven capable of inducing apoptosis on various cancer cells. [6][7][8] However, the potential effect of ethanol and ethyl acetate CZ extract which one is more effective on T47D cells apoptosis remain unclear.…”

“…Curcuma zedoaria is also known as white turmeric, family of Zingiberacea contain various chemical elements such as sesquiterpene, phenylpropanoids, flavonoids, and terpenoids. 6 Based on these constituents, CZ possess several medical benefits such as antioxidant, anti-inflammation, antimicrobial, anti-angiogenesis, and antitumor activities. 9 Amongst these chemical components, polyphenol and flavonoids are the most prominence substance which is utilized widely as cancer chemoprevention and adjuvant therapy.…”