Effect of Wheatgrass on DNA Damage, Oxidative Stress Index and Histological Findings in Diabetic Rats

Abstract

MIS, L.; COMBA, B.; USLU, S. & YELTEKIN, A. Effect of wheatgrass on DNA damage, oxidative stress index and histological findings in diabetic rats. Int. J. Morphol., 36(4):1235Morphol., 36(4): -1240Morphol., 36(4): , 2018. SUMMARY:This study was aimed to search the effect of wheatgrass on the Total Antioxidan (TAS)-Oxidan Status (TOS) and DNA damage in rat with diabetes. The rats used in the study were randomly divided into 4 groups that each of has 10 rats: Control group; 1 ml single dose phosphate-citrate buffer injected i.p (pH: 4.5), Diabetes group; 45 mg/kg single dose streptozotocin injected i.p., Wheatgrass group; was given oral wheatgrass (10 ml/kg/day) for 6 weeks, Diabetes +Wheatgrass group; 45 mg/kg single dose streptozotocin injected i.p. and wheatgrass (10 ml/kg/day) was given by oral during 6 weeks. After the process of experiment during 6 weeks, blood sample and pancreas tissue were taken. The analysis were done of blood glucose levels, TAS, TOS levels by colorimetric kits; DNA damage by ELISA kits in serum. The pancreas tissues were examined histopathologically. In the group of Diabetes+Wheatgrass was determined that the levels of glucose levels (p<0.001),TOS (p<0.05) and OSI (p<0.01) statistically decreased and heal histopatolojical compared to diabetes group. In the group of Wheatgrass was determined that the levels of TAS p<0.05 statistically increased from other groups. The statistical significance were not found in the level of serum 8OHdG differences between the groups. The beta cells were seen to increase in the group receiving wheatgrass for therapeutic purposes.As a conclusion, it was determined that wheatgrass strengthened the anti-oxidant defense system and reduced the glucose level in diabetic rats.

Flavonoid‐rich wheatgrass ( Triticum aestivum L.) diet attenuates diabetes by modulating antioxidant genes in streptozotocin‐induced diabetic rats

“…Also, luteolin contributes to renal protection by increasing HO-1 expression and elevating antioxidants in diabetic nephropathy (Wang et al, 2011). In an analogous study oral treatment of wheatgrass extract for 6 weeks protected the pancreases in STZ-induced diabetic mice (Mis et al, 2018).…”

Vitamins in Cereals: A Critical Review of Content, Health Effects, Processing Losses, Bioaccessibility, Fortification, and Biofortification Strategies for Their Improvement

“…Recently, cereal seedling juice, and particularly wheatgrass juice, has received substantial attention as it is high in nutrients. Wheatgrass is known to be a potent medicine in the treatment of cardiovascular diseases ( 11 ), ulcerative colitis ( 12 ), thalassemia ( 13 ), hepatic injury ( 14 ), diabetes ( 15 ), and cancer ( 16 ); and in addition, it may also provide improved immunity ( 17 ). However, there are limited studies on their vitamin content, which poses a challenge for widespread utilization.…”

Oxidative Stress Caused by Lithium Exposure in the Carassius auratus (goldfish) Liver Tissue

“…Lithium enhances oxidative damage, which is further supported by the fact that it causes a decrease in AchE activity. The levels of MDA oxidant biomarkers and GSHPx, CAT, SOD, antioxidants have been suggested to determine the presence of oxidative stress free radicals or oxidative biomarkers (Mis L et al 2018). Lithium triggers oxidative damage such as superoxide and nitric oxide by creating reactive oxygen species in the liver.…”