Effect of vitamins C and E on oxidative processes in human erythrocytes

Abstract

The oxidative action of 1 mmol l(-1) phenylhydrazine hydrochloride (PH) was studied on human erythrocytes treated with the antioxidants vitamin C (vit. C) and vitamin E (vit. E). The erythrocytes were resuspended in PBS to obtain 35% cell packed volume, and then submitted to the oxidative action of PH for 20 min, with or without previous incubation for 60 min with vit. C or vit. E. Heinz bodies and methemoglobin formation by PH were inhibited in the presence of vit. C. At the concentration of 90 mmol l(-1), vit. C, not only seemed to lose its antioxidant effect, but it also promoted an increase in methemoglobin formation. Vit. C (0.5-80 mmol l(-1)) did not protect against GSH depletion by PH. Vit. C alone produced insignificant hemolysis, but, in the presence of PH, the hemolysis indices were more accentuated. Heinz body formation by PH was inhibited in the presence of vit. E. Formation of methemoglobin induced by PH was decreased by vit. E (0.1-2 mmol l(-1)), although vit. E (3-80 mmol l(-1)) did not lower the concentration of methemoglobin and did not lead to the recovery of the GSH depleted by PH. The results obtained suggest that vit. C and vit. E contribute to the decrease in oxidative stress caused by PH.

Anti-anemic potential estimation on mice and characterization of flavonoids using high performance thin layer chromatography in Wrightia tinctoria bark fraction

“…Vitamins C and E contribute to the decrease in oxidative stress caused by PHZ in vitro by inhibiting Heinz bodies and methemoglobin formation. [38] Flavonoids are a broad class of plant phenolics that are known to possess a well-established protective effect against membrane lipoperoxidative damages. Oxidative damage of erythrocytes has been implicated in the reduced survival of erythrocytes during Leishmanial infection.…”

Metabolic pathways that correlate with post-transfusion circulation of stored murine red blood cells

“…Alpha-tocopherol does have the ability to inhibit chemical oxidation of RBCs; 30 however, the extent to which such is a normal pathway during RBC storage is unclear and has only been tested in an in vitro setting. 31 Of interest, it has recently been reported that vitamin C and N-acetylcysteine mitigate oxidative stress in in vitro human RBC studies.…”

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“…Vitamin E, a constituent of the plasma membrane, then an effective antioxidant, scavenges peroxide free radicals and converts them to less toxic lipid hydroperoxides, thereby protecting the cell membrane and decreasing hemolysis (Niki and Noguchi, 2004). The benefits of this vitamin have been reported in human RBC when challenged with phenylhydrazine hydrochloride (Claro et al, 2006). However, according to Mendanha et al, (2012), AA and α-tocopherol are able to prevent oxidative hemolysis and TBARS production, but can not decrease the membrane rigidity when RBCs are exposed to H 2 O 2 .…”