Effect of vitamin D3 on immunity and antioxidant capacity of pearl oyster Pinctada fucata martensii after transplantation: Insights from LC–MS-based metabolomics analysis

Yang, Chuangye; Du, Xin; Hao, Ruijuan; Wang, Qingheng; Deng, Yuewen; Sun, Ruijiao

doi:10.1016/j.fsi.2019.09.017

Cited by 54 publications

(23 citation statements)

References 48 publications

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“…Hence, dietary requirements for commercial farms might not be catered for by this process. Since 2015, our lab has been developing formulated diets of P. f. martensii (Yang et al, 2017a;Yang et al, 2017b;Yang et al, 2018a;Yang et al, 2018b;Yang et al, 2019a;Yang et al, 2019b) and proven that land-based cultures can boost survival and retention rates of host pearl oysters. In the present research, land-based cultured EG3 showed heightened pearl production (survival rate, retention rate, highquality pearl rate, pearl thickness, and nacre deposition rates).…”

Section: Discussionmentioning

confidence: 99%

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Integrated GC–MS- and LC–MS-Based Untargeted Metabolomics Studies of the Effect of Vitamin D3 on Pearl Production Traits in Pearl Oyster Pinctada fucata martensii

Yang

Zeng

Liao

et al. 2021

Front. Mol. Biosci.

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Pearl oyster Pinctada fucata martensii is widely recognized for biomineralization and has been cultured for high-quality marine pearl production. To ascertain how dietary vitamin D3 (VD3) levels affect the features of pearl production by P. f. martensii and discover the mechanisms regulating this occurrence, five experimental diets with variable levels of VD3 were used with inclusion levels of 0, 500, 1,000, 3,000, and 10,000 IU/kg. The distinct inclusion levels were distributed into five experimental groups (EG1, EG2, EG3, EG4, and EG5). All the experimental groups were reared indoors except the control group (CG) reared at the sea. Pearl oysters, one year and a half old, were used in the grafting operation to culture pearls. During the growing period that lasted 137 days, EG3 had the highest survival rate, retention rate, and high-quality pearl rate. A similar trend was found for EG3 and CG with significantly higher pearl thickness and nacre deposition rates than other groups, but no significant differences were observed between them. A metabolomics profiling using GC–MS and LC–MS of pearl oysters fed with low quantities of dietary VD3 and optimal levels of dietary VD3 revealed 135 statistically differential metabolites (SDMs) (VIP > 1 and p < 0.05). Pathway analysis indicated that SDMs were involved in 32 pathways, such as phenylalanine metabolism, histidine metabolism, glycerophospholipid metabolism, alanine aspartate and glutamate metabolism, arginine and proline metabolism, glycerolipid metabolism, amino sugar and nucleotide sugar metabolism, and tyrosine metabolism. These results provide a theoretical foundation for understanding the impacts of VD3 on pearl production traits in pearl oyster and reinforce forthcoming prospects and application of VD3 in pearl oyster in aquaculture rearing conditions.

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Section: Discussionmentioning

confidence: 99%

“…Organisms were fed one of five experimental treatments, consisting of different dietary levels of VD3 (0, 500, 1,000, 3,000, and 10,000 IU/kg) according to Yang et al (2019b) and the feeds were kept at −20°C until use.…”

Section: Rearing and Experimental Dietsmentioning

confidence: 99%

Integrated GC–MS- and LC–MS-Based Untargeted Metabolomics Studies of the Effect of Vitamin D3 on Pearl Production Traits in Pearl Oyster Pinctada fucata martensii

Yang

Zeng

Liao

et al. 2021

Front. Mol. Biosci.

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“…According to previous reports and the manufacturer’s instructions, the metabolites of cells in induced sputum were assayed using an Q Exactive Orbitrap LC-MS/MS system [ 35 – 37 ]. Biotree Biotech Co., Ltd. (Shanghai, China) helped with the LC-MS analysis.…”

Section: Methodsmentioning

confidence: 99%

“…For the metabolomics analysis, mass spectrometry (MS) raw data files were converted to mzXML format using ProteoWizard software (version 3.0.19282) and processed with R software package XCMS (version 3.2) for peak detection, extraction, alignment, and integration [ 35 , 42 , 43 ]. The peaks were detected, and metabolites could be identified through the interquartile range denoising method.…”

Section: Methodsmentioning

confidence: 99%

Induced sputum metabolomic profiles and oxidative stress are associated with chronic obstructive pulmonary disease (COPD) severity: potential use for predictive, preventive, and personalized medicine

Zhu

Wang

et al. 2020

EPMA Journal

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Chronic obstructive pulmonary disease (COPD) is a highly heterogeneous disease, and metabolomics plays a hub role in predictive, preventive, and personalized medicine (PPPM) related to COPD. This study thus aimed to reveal the role of induced sputum metabolomics in predicting COPD severity. In this pilot study, a total of 20 COPD patients were included. The induced sputum metabolites were assayed using a liquid chromatography-mass spectrometry (LC-MS/MS) system. Five oxidative stress products (myeloperoxidase (MPO), superoxide dismutase (SOD), glutathione (GSH), neutrophil elastase (NE), and 8-iso-PGF2α) in induced sputum were measured by ELISA, and the metabolomic profiles were distinguished by principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA). The Kyoto Encyclopedia of Genes and Genomes (KEGG) was used for pathway enrichment analysis, and a significant difference in induced sputum metabolomics was observed between moderate and severe COPD. The KEGG analysis revealed that the glycerophospholipid metabolism pathway was downregulated in severe COPD. Due to the critical role of glycerophospholipid metabolism in oxidative stress, significant negative correlations were discovered between glycerophospholipid metabolites and three oxidative stress products (SOD, MPO, and 8-iso-PGF2α). The diagnostic values of SOD, MPO, and 8-iso-PGF2α in induced sputum were found to exhibit high sensitivities and specificities in the prediction of COPD severity. Collectively, this study provides the first identification of the association between induced sputum metabolomic profiles and COPD severity, indicating the potential value of metabolomics in PPPM for COPD management. The study also reveals the correlation between glycerophospholipid metabolites and oxidative stress products and their value for predicting COPD severity.

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“…Pearl oysters as warmwater shellfish species were cultured in natural seas and exposed in diverse temperature ranges and their corresponding effects (Adzigbli et al, 2020b). Pinctada fucata martensii, as the main marine pearl called "South China sea pearls" production in China (Yang et al, 2019;Zhang et al, 2021), hold a low enduring capacity to extreme temperatures and just hold an optimal range of 23-28 • C (Deng et al, 2010). During pearl culture, the juvenile of pearl oyster is transferred to a culturing site in the sea and thus exposed to various environmental perturbations that affect its survival and growth in the natural sea (Hao et al, 2019;Adzigbli et al, 2020b).…”

Section: Introductionmentioning

confidence: 99%

Identification and Allelic Variants Associated With Cold Tolerance of PmPIAS in Pinctada fucata martensii

et al. 2021

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The protein inhibitor of activated STAT (PIAS) functions in diverse aspects, including immune response, cell apoptosis, cell differentiation, and proliferation. In the present study, the PIAS in the pearl oyster Pinctada fucata martensii was characterized. The sequence features of PmPIAS were similar to that of other PIAS sequences with PIAS typical domains, including SAP, Pro-Ile-Asn-Ile-Thr (PINIT), RLD domain, AD, and S/T-rich region. Homologous analysis showed that PmPIAS protein sequence showed the conserved primary structure compared with other species. Ribbon representation of PIAS protein sequences also showed a conserved structure among species, and the PINIT domain and RLD domain showed the conserved structure compared with the sequence of Homo sapiens. The expression pattern of PmPIAS in different tissues showed significant high expression in the gonad. PmPIAS also exhibited a significantly higher expression in the 1 and 2 days after cold tolerance stress (17°C) and showed its potential in the cold tolerance. The SNP analysis of the exon region of PmPIAS obtained 18 SNPs, and among them, 11 SNPs showed significance among different genotypes and alleles between cold tolerance selection line and base stock, which showed their potential in the breeding for cold tolerance traits.

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Effect of vitamin D3 on immunity and antioxidant capacity of pearl oyster Pinctada fucata martensii after transplantation: Insights from LC–MS-based metabolomics analysis

Cited by 54 publications

References 48 publications

Integrated GC–MS- and LC–MS-Based Untargeted Metabolomics Studies of the Effect of Vitamin D3 on Pearl Production Traits in Pearl Oyster Pinctada fucata martensii

Integrated GC–MS- and LC–MS-Based Untargeted Metabolomics Studies of the Effect of Vitamin D3 on Pearl Production Traits in Pearl Oyster Pinctada fucata martensii

Induced sputum metabolomic profiles and oxidative stress are associated with chronic obstructive pulmonary disease (COPD) severity: potential use for predictive, preventive, and personalized medicine

Identification and Allelic Variants Associated With Cold Tolerance of PmPIAS in Pinctada fucata martensii

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