Identification and Allelic Variants Associated With Cold Tolerance of PmPIAS in Pinctada fucata martensii

Abstract

The protein inhibitor of activated STAT (PIAS) functions in diverse aspects, including immune response, cell apoptosis, cell differentiation, and proliferation. In the present study, the PIAS in the pearl oyster Pinctada fucata martensii was characterized. The sequence features of PmPIAS were similar to that of other PIAS sequences with PIAS typical domains, including SAP, Pro-Ile-Asn-Ile-Thr (PINIT), RLD domain, AD, and S/T-rich region. Homologous analysis showed that PmPIAS protein sequence showed the conserved primary structure compared with other species. Ribbon representation of PIAS protein sequences also showed a conserved structure among species, and the PINIT domain and RLD domain showed the conserved structure compared with the sequence of Homo sapiens. The expression pattern of PmPIAS in different tissues showed significant high expression in the gonad. PmPIAS also exhibited a significantly higher expression in the 1 and 2 days after cold tolerance stress (17°C) and showed its potential in the cold tolerance. The SNP analysis of the exon region of PmPIAS obtained 18 SNPs, and among them, 11 SNPs showed significance among different genotypes and alleles between cold tolerance selection line and base stock, which showed their potential in the breeding for cold tolerance traits.

Molecular characterization and SNPs association with growth‐related traits of myosin heavy chains from the pearl oyster Pinctada fucata martensii

“…In the present study, 18 SNPs from PmMYH were found to be strongly associated Note: Mean values with the same letter within a column are not significant different (p > 0.05). Lai et al, 2021). Of these associated SNPs, two cross-tested in the golden selection line and base stock, further indicating its potential in growth trait construction.…”

“…In the present study, 18 SNPs from PmMYH were found to be strongly associated with growth traits, indicating its potential in growth selection. Several SNPs such as SNP 31, 57, 85 and 89 lack one of the three possible genotypes, which indicated that these SNP genotypes disappearance were the consequence of local adaptation or selection (Du et al, 2014; Lai et al, 2021). Of these associated SNPs, two cross‐tested in the golden selection line and base stock, further indicating its potential in growth trait construction.…”

“…Therefore, several studies have been investigated the potential genetic mechanism of rapid growth of pearl oysters (Deng et al, 2011). Single nucleotide polymorphisms (SNPs) for candidate genes of economically important traits are identified to obtain markers for breeding aquatic animals (Guo et al, 2011(Guo et al, , 2012Hao et al, 2020;Lai et al, 2021;Lei et al, 2019;Li et al, 2018). In this study, the MYH gene from P. f. martensii was cloned and analysed to explore its potential use in breeding for faster growth of pearl oyster.…”

Molecular cloning, characterization, and expression of two 5-HTRs from the pearl oyster Pinctada fucata martensii

“…Quantitative real-time RT-PCR (qRT-PCR) was performed with b-actin as the reference gene to determine mRNA expression. Research has showed that b-actin was the most stable reference gene for different tissues and different embryonic developmental stages (Wang and He, 2013) and have been widely used as reference genes for qRT-PCR analysis (Wang et al, 2019;Lai et al, 2021;Chen et al, 2022). qRT-PCR was performed with SYBR Green Master Mix (Thermo-Fisher Scientific, USA) according to the protocol recommended by the manufacturer.…”