Effect of Various Immersion Time and Water Temperature on Seed Germination of Clitoria ternatea and Momordica charantia

Abstract

The seeds of Clitoria ternatea and Momordica charantia were subjected to seven pre-sowing treatments, i.e., control (T0), peeled coat and soaked in 5 oC for 24 hours (T1), peeled coat and soaked in 37 oC for 24 hours (T2), peeled coat and soaked in 5 oC for 48 hours (T3), peeled coat and soaked in 37 oC for 48 hours (T4), peeled coat and soaked in 5 oC for 48 hours (T5), peeled coat and soaked in 37 oC for 72 hours (T6). The study revealed that peeling the coat and soaking seeds in water for various temperatures and periods improved seed germination. The highest germination and germination energy percentage of C. ternatea were observed in T2, namely 94.95% and 23.69%, respectively, while the lowest germination (0%) and germination energy (0%) was found in T6. The highest germination and germination energy percentage of M. charantia, namely 64.38%, and 16.10%, respectively, were found in T3, while the lowest germination (10.67%) and germination energy (2.17%) were observed in T0. The germination may vary for both seeds used in the study as C. ternatea and M. charantia are different in type. The pre-sowing treatments of seeds would prove its potential in the practical fields.

Factors Affecting Telang (Clitoria ternatea L.) Germination: Systematic Literature Review

“…The effect of dormancy and its alleviation method on C. ternatea germination is also recorded in publications in this SLR study. Mechanical scarification, alone or followed with water immersion, is mentioned to improve C. ternatea germination (Campbell et al, 2022;Md Salleh & Pa'ee, 2021;Nagar & Meena, 2015). Chemical scarification using various concentrations and immersion duration of sulphuric acid (H2SO4) is another treatment already used in C. ternatea (Makasana et al, 2016;Nagar & Meena, 2015;Pincay-Ganchozo et al, 2021;Reino et al, 2011;Shobharani & Sundareswaran, 2018).…”

“…In C. ternatea, mechanical scarification followed by 24 hours of immersion in water is reported to improve the plant seed germination compared to control and mechanical scarification alone (Campbell et al, 2022). However, 72 hours of water immersion following mechanical scarification reduces seed germination (Md Salleh & Pa'ee, 2021). Meanwhile, 10-90 minutes of immersion of the plant species seed in concentrated H2SO4 improves its germination than the shorter treatment period (Makasana et al, 2016).…”

Effects of Microwave Power and Carrier Materials on Anthocyanins, Antioxidants, and Total Phenolic Content of Encapsulated Clitoria ternatea Flower Extract

“…Additionally, the Clitoria ternatea flower has been widely used in traditional medicine, particularly as a supplement to enhance cognitive functions and alleviate symptoms of numerous ailments, including fever, inflammation, pain, and diabetes (Mukherjee et al, 2008). The blue pea flower is very popular among traditional Chinese and Ayurvedic medicine and has been consumed for centuries as a memory enhancer, brain booster, antistress and insomnia (Anthika et al, 2015;Salleh & Pa'ee, 2021;Mukherjee et al, 2008;Verma et al, 2013). The major phytoconstituents found in extracts from Clitoria ternatea flowers are pentacyclic triterpenoids such as taraxerol and taraxerone (Swathi et al, 2021).…”