Effect of two different preparations of platelet-rich plasma on synoviocytes

Abstract

PurposeTo analyse the modifications induced by two different platelet-rich plasma (PRP) preparations on osteoarthritis (OA) synoviocytes, by documenting changes in gene expression of factors involved in joint physiopathology.MethodsOA synoviocytes were cultured for 7 days in medium with different concentrations of either P-PRP (a pure platelet concentrate without leucocytes but with a limited number of platelets), L-PRP (a higher platelet concentrate containing leucocytes) or platelet-poor plasma (PPP). Gene expression of interleukin (IL)-1beta, IL-6, IL-8/CXCL8, tumour necrosis factor alpha, IL-10, IL-4, IL-13, metalloproteinase-13, tissue inhibitor of metalloproteinase (TIMP)-1, (TIMP)-3, (TIMP)-4, vascular endothelial growth factor, transforming growth factor beta1, fibroblast growth factor (FGF)-2, hepatocyte growth factor (HGF), hyaluronic acid (HA) synthases (HAS)-1, (HAS)-2, and (HAS)-3 was analysed by RT-PCR. HA production was determined in culture supernatants by ELISA.ResultsIL-1β, IL-8 and FGF-2 were significantly induced by L-PRP compared to both P-PRP and PPP; HGF was down-modulated by L-PRP versus both P-PRP and PPP, and an inverse dose–response influence was shown for all preparations. Expression level of TIMP-4 was lower in the presence of L-PRP compared with P-PRP. HA production and HAS gene expression did not seem to be modulated by PRP.ConclusionsL-PRP is able to sustain the up-regulation of proinflammatory factors, (IL-1beta, IL-8 and FGF-2), together with a down-modulation of HGF and TIMP-4 expression, two factors that have been recognized as anti-catabolic mediators in cartilage, thus supporting the need to further optimize the PRP preparations to be applied in clinical practice.

Evaluation of the anti-inflammatory effects of two platelet-rich gel supernatants in an in vitro system of cartilage inflammation

“…As mentioned, there are controversial opinions regarding which is the ideal concentration of PLT and WBC in PRP to be used for therapeutic aims in patients with OA [20]. This in vitro research was conducted to complement previous studies [22,[24][25][26] of in vitro cartilage response to several concentrations (25% and 50%) of PRG supernatants from two different PRP preparations (P-PRP and L-PRP).…”

“…These complex mechanisms are constantly reported in several studies evaluating the in vitro effects of several PRP preparations and related substances in in vitro systems of joint cell (tissue) inflammation [24,26,34,36].…”

“…Some studies have indicated that PRP preparations poor in white blood cells (WBCs) -also known as pure PRP (P-PRP) or leukoreduced PRPhave more beneficial (anabolic) effects than PRP preparations rich in WBC (also known as leukocyte-and platelet-rich plasma [L-PRP] or leukoconcentrate PRP) [22,[24][25][26].…”

Antiviral Resistance of Splenocytes in Aged Mice

“…Mariani et al (2015) tested the effect of PRP against Escheri chia coli, Staphylococcus aureus, Klebsiella pneumoniae, Pseudomonas aeruginosa and Enterococcus faecalis, as species potentially involved in bone, soft tissue and wound infections (Zalavras et al, 2004). In the current study, we have selected four microorga nisms that are commonly found in oral and periodontal infections and investigated whether PRP could have in vitro antimicrobial effects against them.…”

The Differential Effects of Leukocyte-Containing and Pure Platelet-Rich Plasma on Nucleus Pulposus-Derived Mesenchymal Stem Cells: Implications for the Clinical Treatment of Intervertebral Disc Degeneration

“…The exclusion of leukocytes has been proven more effective when applied in the treatment of bone defect [29], osteoarthritis [35], and acute tendon injury [36]. In this study, similar platelet concentrations of P-PRP and L-PRP resulted in significantly different ECM production in each individual group.…”