Antiviral Resistance of Splenocytes in Aged Mice

Abstract

A b s t r a c tAntimicrobial properties of platelet rich plasma (PRP) against various microorganisms have been recently pointed out. PRP could be an alternative to conventional antibiotics in preventing oral and periodontal infections. We examined whether PRP has in vitro antimicrobial properties against Aggregatibacter actinomycetemcomitans, Prophyromonas gingivalis, Staphylococcus aureus and Candida albicans. PRP and platelet-poor plasma (PPP) were obtained from whole blood of 10 healthy volunteers and 10 periodontitis patients. In vitro laboratory susceptibility was carried out using the modified agar diffusion method by measuring the diameters of inhibition zones on agar plates coated with selected microbial strains. Both calcium chloride (CaCl2) activated and non activated samples were tested. Both activated PRP and PPP, of both patients and controls, effectively inhibited the growth of A. actinomycetemcomitans, P. gingivalis and C. albicans. However, a statistically significant difference in favor of PRP was found indicating more susceptibility to PRP than PPP (p < 0.05). Non activated PRP and PPP exhibited negative zones of inhibition against the studied microorganisms. There was no activity against S. aureus. No statistically significant difference was found between the antimicrobial effects of PRP and/or PPP obtained from patients and controls (p > 0.05). We conclude that PRP is a potentially useful substance against oral and periodontal pathogens. Activated PRP was found to be more active than activated PPP and the activation of coagulation is a fundamental step. Additionally, the antimicrobial activity of PRP and/or PPP seems not to be affected by periodontitis.K e y w o r d s: antimicrobial activity, oral infection, platelet-rich plasma

Antimicrobial Effect of Platelet-Rich Plasma againstPorphyromonas gingivalis

“…The capacity of P. gingivalis inhibition of PRP in our results was similar to those of other studies using the agar diffusion [6, 20]. In addition, Aggour and Gamil conclude that there is no difference in bacterial resistance between the healthy group and periodontitis group [20].…”

“…This is the classic method applied in studies using PRP [19]. In Table 1, the platelet counts in the two groups after enrichment increased from 2.7 to 5.1 times that in the total blood, which is consistent with many studies [4, 11, 20]. The preenrichment platelets (total blood) between the two groups were not significantly different.…”

Application of platelet-rich plasma and platelet lysate as blood biomaterials in the treatment of lymphocutaneous sporotrichosis

“…To the best of our knowledge, this study is the only experimental research addressing the effect of PRP and platelet lysate against fungi. Only Yung et al and Aggour et al have investigated the antimicrobial effect of PRP against periodontal pathogens in vitro [18, 21]. The results of both studies were suggestive of the inhibitory role of PRP against bacterial and fungal ( Candida ) growth.…”

Application of a collagen scaffold saturated with platelet-rich plasma in prevention of bisphosphonate-related osteonecrosis of the jaw in the rat animal model

“…On the other hands, PRP can also obstruct the progression of each of these factors. Aggour and Gamil (2017) have assessed antimicrobial effects of PRP against selected oral pathogens. El-Sharkawy et al (2007) have assessed the anti-inflammatory potential of PRP and stated that PRP significantly suppresses Monocyte Chemotactic Pretein-1 (MCP-1) and therefore promotes changes in monocyte-mediated proinflammatory cytokine and chemokine release which leads to restraint of inflammation (El-Sharkawy et al, 2007).…”