Antimicrobial Effect of Platelet-Rich Plasma againstPorphyromonas gingivalis

Abstract

Aim. The aim of our study was to evaluate whether there was a difference in antimicrobial effect between the PRP of healthy volunteers and that of patients with chronic periodontitis against P. gingivalis, which was fresh cultured from subgingival plaque. Methods. Subgingival plaque of patients with moderate and severe chronic periodontitis was collected to isolate P. gingivalis. The PRP of four individuals with healthy periodontium and four patients with moderate and severe periodontitis were collected with a specific kit using a two-centrifuge procedure, and then, the antibacterial properties against P. gingivalis were tested, through their minimum inhibitory concentration (MIC), adhesion resistance assay, and biofilm susceptibility assay. Results. P. gingivalis was successfully isolated from the subgingival plaque of the 21st patient. The round, smooth, and black colony appeared in the agar disk after 7–10 days of incubation under anaerobic conditions. Bacterial identification was performed by MALDI-TOF and confirmed by PCR. All PRP samples tested showed the ability to inhibit P. gingivalis growth. The MIC value (expressed as fraction of PRP) was 1/2, and PRP prevented P. gingivalis attachment on the disk surface. However, PRP did not have a strong effect on the suppression of P. gingivalis biofilm. Conclusion. PRP of individuals with healthy periodontium and chronic periodontitis patients showed antibacterial properties against P. gingivalis. This material can become an adjunct to periodontal treatment.

Current art of combination therapy with autologous platelet‐rich plasma for stable vitiligo: A meta‐analysis

“…Potential favourable effects of leucocytes may be attributed to their roles in tissue remodelling, antibacterial affects, and regulation of immune responses 35 . Topical application or intradermal injection of PRP allow biomolecules to be delivered to target tissues, which effectively regulate some essential functions in the local microenvironment: proliferation, migration, and differentiation of stem cells (eg, dermal fibroblasts, epithelial cells, and endothelial cells), new collagen and matrix formation, modulation of inflammatory and immune responses, and angiogenesis, as well as antimicrobial effects 5,36‐40 . These healing and regenerative properties pave the way that PRP may be a potentially effective biomaterial in vitiligo treatment.…”

“…35 Topical application or intradermal injection of PRP allow biomolecules to be delivered to target tissues, which effectively regulate some essential functions in the local microenvironment: proliferation, migration, and differentiation of stem cells (eg, dermal fibroblasts, epithelial cells, and endothelial cells), new collagen and matrix formation, modulation of inflammatory and immune responses, and angiogenesis, as well as antimicrobial effects. 5,[36][37][38][39][40] These healing and regenerative properties pave the way that PRP may be a potentially effective biomaterial in vitiligo treatment. Recently, some researchers have begun to combine PRP with several conventional treatments for vitiligo in comparison to monotherapy, but inconsistent results were reported.…”

Platelet Concentrates in Musculoskeletal Medicine

“…This approach allows us to deliver biomolecules released by a concentrated pool of activated platelets to the target tissue site of injury, thus effectively contributing to the modulation of inflammatory process, angiogenesis and immune response, as well as promoting the healing and repair of injured tissues [5,6]. Moreover, biological blood-derived products have been recognized to have antimicrobial effects, such as being able to inhibit and/or to inactivate different bacterial strains [6][7][8].…”

“…Neutrophils are essential for killing bacteria and other microorganisms [86]. Since platelets also contribute to the antibacterial response [6][7][8], leukocytes may synergize with platelets and potentiate PRP antimicrobial effects.…”

Quercetin inhibits virulence properties of Porphyromas gingivalis in periodontal disease

“…Two major etiological factors of periodontal disease are specific bacterial species called periodontopathogens colonizing subgingival sites, and subsequent host inflammatory and immune responses to these periodontopathogens 5 . Although over 700 microbial species have been identified in subgingival plaque, Porphyromonas gingivalis , a black-pigmented, rod-shaped, and Gram-negative anaerobic bacterium, is a keystone pathogen for periodontal disease 6 .…”