Effect of triton X-100 on alkaline lipase production by Pseudomonas pseudoalcaligenes F-111

Lin, Shuen-Fuh; Chiou, Chien-Ming; Tsai, Ying-Chieh

doi:10.1007/bf00127434

Cited by 52 publications

(36 citation statements)

References 13 publications

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“…Other compounds, such as Tweens, have been shown to stimulate the production of lipase in Acinetobacter and Pseudomonas species (12,24,26). To determine whether such compounds stimulate lipase production in B. glumae as well, strains PG1 and LU8093 were grown with 10% hexadecane or tetradecane, 1% gum arabic, or 0.1% of the nonionic detergents Tween 80 and Triton X-100.…”

Section: Resultsmentioning

confidence: 99%

“…We investigated the possibilities for increasing the production of lipase in this strain even further by modifying the growth medium. Several compounds, including alkanes, gum arabic, spans, and Tweens, have been shown to increase lipase production in other bacteria, such as A. calcoaceticus and Pseudomonas species (12,17,18,(24)(25)(26)35). Indeed, we found that additives, such as hexadecane, Triton X-100, and Tween 80, considerably improved productivity.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we investigated whether we could improve lipase production even further by including various additives, such as hexadecane and detergents, in the growth medium. Previously, such compounds have been found to stimulate lipase production in other bacteria (12,17,18,(24)(25)(26)35), but which specific step in lipase production they stimulate is not exactly known. We demonstrate that these additives affect lipase production in B. glumae at various stages.…”

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confidence: 99%

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Hexadecane and Tween 80 Stimulate Lipase Production in Burkholderia glumae by Different Mechanisms

Boekema

Beselin

Breuer

et al. 2007

Appl Environ Microbiol

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Burkholderia glumae strain PG1 produces a lipase of biotechnological relevance. Lipase production by this strain and its derivative LU8093, which was obtained through classical strain improvement, was investigated under different conditions. When 10% hexadecane was included in the growth medium, lipolytic activity in both strains could be increased ϳ7-fold after 24 h of growth. Hexadecane also stimulated lipase production in a strain containing the lipase gene fused to the tac promoter, indicating that hexadecane did not affect lipase gene expression at the transcriptional level, which was confirmed using lipA-gfp reporter constructs. Instead, hexadecane appeared to enhance lipase secretion, since the amounts of lipase in the culture supernatant increased in the presence of hexadecane, with a concomitant decrease in the cells, even when protein synthesis was inhibited with chloramphenicol. In the presence of olive oil as a carbon source, nonionic detergents, such as Tween 80, increased extracellular lipase activity twofold. Like hexadecane, Tween 80 appeared to stimulate lipase secretion, although in a more disruptive manner, since other, normally nonsecreted proteins were found in the culture supernatant. Additionally, like olive oil, Tween 80 was found to induce lipase gene expression in strain PG1 in medium containing sucrose as a carbon source but not in glucose-containing medium, suggesting that lipase gene expression is prone to catabolite repression. In contrast, lipase production in the lipaseoverproducing strain LU8093 was independent of the presence of an inducer and was not inhibited by glucose. In conclusion, hexadecane and Tween 80 enhance lipase production in B. glumae, and they act via different mechanisms.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Hexadecane and Tween 80 Stimulate Lipase Production in Burkholderia glumae by Different Mechanisms

Boekema

Beselin

Breuer

et al. 2007

Appl Environ Microbiol

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“…However, irrespective of inducing compounds added to the medium, both the activity of lipase and bacterial growth were increased when lipids were added to the medium, indicating that they not only acted as lipase inducers, but could also be utilized as energy and carbon sources. Tweens have been shown to stimulate the production of lipase in Acinetobacter and Pseudomonas species (Lin et al 1995;Martinez and Nudel 2002). Olive oil was present in the growth medium as a carbon source.…”

Section: Effect Of Different Substrates On Lipase Productionmentioning

confidence: 99%

Characterization of Lipase from Bacillus subtilisI-4 and Its Potential Use in Oil Contaminated Wastewater

Iqbal

Rehman

2015

Braz. arch. biol. technol.

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“…Enzyme activity was assayed spectrophotometrically using p-nitrophenyllaurate (p-NPL) as substrate, according to Lin et al (1995). Incubations were performed at 40ºC for 15 minutes and the release of p-nitrophenol was measured at 410 nm using a DU-640 spectrophotometer (Beckman Coulter).…”

Section: Lipase Detection Assay Of Esterase Activitymentioning

confidence: 99%

Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.

Koblitz

Pastore

2006

Ciênc. agrotec.

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The present study had as a goal to purify and characterize the lipolytic fraction secreted by a strain of Rhizopus sp. Only 3 steps of purification were necessary to achieve SDS-PAGE homogeneity. One band with 37.5 KDa molecular mass and with 1446 U/ mg specific activity was obtained. The purified fraction presented 2 lipase isoforms; both showed optimum activity at 50ºC, and were stable between 6.5 and 7.5 pH values and at temperatures below 50ºC and also kept their activity in hexane. The lipase was inactivated by Hg +2 and by n-bromosuccinimide and activated by Na + .Index terms: Lipase, Rhizopus sp., purification, biochemical characterization. RESUMOO presente trabalho teve por objetivo purificar e caracterizar a fração lipolítica secretada por uma linhagem de Rhizopus sp. Apenas 3 etapas de purificação foram necessárias para alcançar homogeneidade em eletroforese de proteína desnaturada. Uma única banda com massa molecular de 37,5 KDa e atividade específica de 1446U/mg foi obtida. A fração purificada apresentou 2 isoformas de lipase, ambas com temperatura ótima de atividade igual a 50ºC, mantiveram-se estáveis entre valores de pH entre 6,5 e 7,5 e a temperaturas inferiores a 50ºC e mantiveram sua atividade na presença de hexano. A fração lipolítica foi inativada por Hg + e por nbromosuccinimida e ativada por Na + .Termos para indexação: Lipase, Rhizopus sp., purificação, caracterização bioquímica.

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Effect of triton X-100 on alkaline lipase production by Pseudomonas pseudoalcaligenes F-111

Cited by 52 publications

References 13 publications

Hexadecane and Tween 80 Stimulate Lipase Production in Burkholderia glumae by Different Mechanisms

Hexadecane and Tween 80 Stimulate Lipase Production in Burkholderia glumae by Different Mechanisms

Characterization of Lipase from Bacillus subtilisI-4 and Its Potential Use in Oil Contaminated Wastewater

Purification and biochemical characterization of an extracellular lipase produced by a new strain of Rhizopus sp.

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