Characterization of Lipase from Bacillus subtilisI-4 and Its Potential Use in Oil Contaminated Wastewater

Iqbal, Syeda Abeer; Rehman, Abdul

doi:10.1590/s1516-89132015050318

Cited by 23 publications

(15 citation statements)

References 31 publications

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“…The amount of oleic acid in the other oils tested in this work is 56% in canola oil and 9% in grape seed oil. Several studies confirm a high production of lipase in culture media in the presence of olive oil at a concentration of 0.1% to 3% (Feitosa et al 2010, Sooch and Kauldhar 2013, Iqbal and Rehman 2015 which corroborate to the results obtained in the present work. Quian and Chun-Yun (2009) in their studies reported a 4% increase in lipase activity with olive oil in the culture medium.…”

Section: Discussionsupporting

confidence: 91%

Production of lipolytic enzymes by bacteria isolated from biological effluent treatment systems

Furini

Berger

Campos

et al. 2018

An. Acad. Bras. Ciênc.

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This work aimed to evaluate the production of lipolytic complexes, produced by microorganisms isolated from a biological treatment system of effluents from a hotel. To select the best lipolytic microorganism for use in biotechnological processes, we tested 45 bacterial isolates recovered from the raw effluent of the hotel's restaurant waste tank. Lipase production was assayed in culture medium supplemented with olive oil and rhodamine B, incubated at 25 °C and 30 °C for 24 h-48 h. Results showed 22 isolates lipase producers. All isolates were inoculated on medium without yeast extract to select the ones with highest enzyme yields. Out of these, nine isolates showed high lipase activity. The strain with the larger halo was assayed in submerged culture using an orbital shaker and a bioreactor, with three different substrates (olive oil, grape seed oil, and canola oil). Isolate G40 identified as Acinetobacter baylyi was selected to run the production assays because it showed the best result in the solid medium. In the bioreactor, maximum lipase production was obtained after 12 h of culture with the three substrates evaluated: 0,358 U/mL.min-1 in olive oil, 0,352 U/mL.min-1 with grapeseed oil, and 0,348 U/mL.min-1 with canola oil.

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Section: Discussionsupporting

confidence: 91%

Production of lipolytic enzymes by bacteria isolated from biological effluent treatment systems

Furini

Berger

Campos

et al. 2018

An. Acad. Bras. Ciênc.

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“…This is due to the presence of oleic acid in large quantities in olive oil (78%) because lipases are of the inductive type with a preference for monounsaturated long chain fatty acids. These results are consistent with several studies on microbial lipases showing a high production of lipase in the presence of olive oil among several oils tested (Feitosa et al, 2010;Sooch and Kauldhar, 2013;Iqbal and Rehman, 2015;Quian and Chun-Yun, 2009;Nwachukwu et al, 2017;Vishnupriya et al, 2010;Esakkiraj et al, 2010;Dandavate et al, 2009). An important lipolytic activity is also observed with 2% olive oil in other bacterial species, like Pseudomonas aeruginosa KM110 and Bacillus sp ZR-5 (Mobarak-Qamsari et al, 2011;Soleymani et al, 2017).…”

Section: Intracellular Lipolytic and Esterase Activitiessupporting

confidence: 91%

Lipase and esterase activities of lactic acid bacteria isolated from different biotopes

Dellali¹,

Karam²,

Karam³

2020

Afr. J. Biotechnol.

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The lipolytic and esterase activities of fifteen strains of lactic acid bacteria isolated from different biotopes of Algeria and Mauritania were tested on MRS medium supplemented with lipidic substrates. Five of them showed maximum activity in the presence of tributyrin; the activity is therefore a tributyrin esterase. These strains were identified by MALDI-TOF in Enterococcus faecium and Enterococcus durans. The study of growth kinetics as a function of time shows a start of fatty acid production during the exponential phase to reach its maximum in the stationary phase. A better esterase activity is observed at between pH 6 and 9 and at an optimal temperature of 30 to 40°C for the five strains. The influence of metal and additive ions on the esterase activity varies between bacteria but generally, total inhibition was observed in all strains tested in the presence of SDS, NaN 3 , CuCl 2 , EDTA, AgNO 3 and HgCl 2 .

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“…Investigations suggest that oil contaminated areas may be of scientific value in obtaining strains for application in environmental biotechnology, such as Aspergillus sp. [36]; Trichoderma and Penicillium [37]; Rhodococcus cercidiphylli [38], and Bacillus subtilis [39].…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

Hydrocarbon-associated substrates reveal promising fungi for poly (ethylene terephthalate) (PET) depolymerization

et al. 2019

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Recalcitrant characteristics and insolubility in water make the disposal of synthetic polymers a great environmental problem to be faced by modern society. Strategies towards the recycling of post-consumer polymers, like poly (ethylene terephthalate, PET) degradation/depolymerization have been studied but still need improvement. To contribute with this purpose, 100 fungal strains from hydrocarbon-associated environments were screened for lipase and esterase activities by plate assays and high-throughput screening (HTS), using short-and long-chain fluorogenic probes. Nine isolates were selected for their outstanding hydrolytic activity, comprising the genera Microsphaeropsis, Mucor, Trichoderma, Westerdykella, and Pycnidiophora. Two strains of Microsphaeropsis arundinis were able to convert 2-3% of PET nanoparticle into terephthalic acid, and when cultured with two kinds of commercial PET bottle fragments, they also promoted weight loss, surface and chemical changes, increased lipase and esterase activities, and led to PET depolymerization with release of terephthalic acid at concentrations above 20.0 ppm and other oligomers over 0.6 ppm. The results corroborate that hydrocarbon-associated areas are important source of microorganisms for application in environmental technologies, and the sources investigated revealed important strains with potential for PET depolymerization.

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Characterization of Lipase from Bacillus subtilisI-4 and Its Potential Use in Oil Contaminated Wastewater

Abstract: A lipase producing bacterium was isolated from oil contaminated effluents of various industries from

Cited by 23 publications

References 31 publications

Production of lipolytic enzymes by bacteria isolated from biological effluent treatment systems

Production of lipolytic enzymes by bacteria isolated from biological effluent treatment systems

Lipase and esterase activities of lactic acid bacteria isolated from different biotopes

Hydrocarbon-associated substrates reveal promising fungi for poly (ethylene terephthalate) (PET) depolymerization

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