Effect of topology of poly(L-lactide-<i>co</i>-<i>ε</i>-caprolactone) scaffolds on the response of cultured human umbilical cord Wharton’s jelly-derived mesenchymal stem cells and neuroblastoma cell lines

Thapsukhon, Boontharika; Daranarong, Donraporn; Meepowpan, Puttinan; Suree, Nuttee; Molloy, Robert; Inthanon, Kewalin; Wongkham, Weerah; Punyodom, Winita

doi:10.1080/09205063.2014.918457

Cited by 9 publications

(17 citation statements)

References 37 publications

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“…This material is highly biodegradable and biocompatible for various cell types, including chondrocytes [ 17 ], osteoblasts [ 18 ], smooth muscle cells [ 19 ], and neural stem cells [ 20 ]. PLCL scaffolds also support adhesion and proliferation of the BCP-K1 (hWJMSC) cell line [ 21 ] and control cardiomyocyte differentiation of bone marrow-derived MSC in rat [ 22 ]. Many matrix types, which combine synthetic and natural materials, are able to enhance biocompatibility and biofunctionality.…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility Assessment of PLCL‐Sericin Copolymer Membranes Using Wharton’s Jelly Mesenchymal Stem Cells

Inthanon

Daranarong

Techaikool

et al. 2015

Stem Cells International

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Stem cells based tissue engineering requires biocompatible materials, which allow the cells to adhere, expand, and differentiate in a large scale. An ideal biomaterial for clinical application should be free from mammalian products which cause immune reactivities and pathogen infections. We invented a novel biodegradable poly(L-lactic-co-ε-caprolactone)-sericin (PLCL-SC) copolymer membrane which was fabricated by electrospinning. Membranes with concentrations of 2.5 or 5% (w/v) SC exhibited qualified texture characteristics with a noncytotoxic release profile. The hydrophilic properties of the membranes were 35–40% higher than those of a standard PLCL and commercial polystyrene (PS). The improved characteristics of the membranes were due to an addition of new functional amide groups, C=O, N–H, and C–N, onto their surfaces. Degradation of the membranes was controllable, depending on the content proportion of SC. Results of thermogram indicated the superior stability and crystallinity of the membranes. These membranes enhanced human Wharton's jelly mesenchymal stem cells (hWJMSC) proliferation by increasing cyclin A and also promoted cell adhesion by upregulating focal adhesion kinase (FAK). On the membranes, hWJMSC differentiated into a neuronal lineage with the occurrence of nestin. These data suggest that PLCL-SC electrospun membrane represents some properties which will be useful for tissue engineering and medical applications.

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Section: Introductionmentioning

confidence: 99%

Biocompatibility Assessment of PLCL‐Sericin Copolymer Membranes Using Wharton’s Jelly Mesenchymal Stem Cells

Inthanon

Daranarong

Techaikool

et al. 2015

Stem Cells International

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“…These results can be explained by entrapped air in the surface pores within the nanofibrous structure of TC-loaded S-PLCL, resulting from the electrospinning process, which can even be used to generate superhydrophobicity. 33,46,47 It is also important to note that these values may have also been influenced by the presence of pores under the water droplets in the various fibrous morphologies, as reported elsewhere. 35,48 This superhydrophobicity property can be beneficial in preventing bacterial adhesion, but is not always sustainable.…”

Section: Resultsmentioning

confidence: 71%

Tetracycline-Loaded Electrospun Poly(l-lactide-co-ε-caprolactone) Membranes for One-Step Periodontal Treatment

Jenvoraphot

Thapsukhon

Daranarong

et al. 2022

ACS Appl. Polym. Mater.

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In this research, a one-step periodontal membrane, with the required function and properties, has been designed as an alternative method of tissue regenerative treatments. Designed nanoporous prototypes from poly(Llactide-co-ε-caprolactone) (PLCL, 70:30 mol %) were fabricated by electrospinning, denoted as S-PLCL. They were subsequently loaded with tetracycline (TC) in order to enhance periodontal regeneration and deliver an antiinflammatory and antibiotic drug. It was found that TC loading did not have any significant effect on the fiber diameter but did increase hydrophilicity. With the increase in TC loading, the water vapor permeability (WVP) of the S-PLCL membrane decreased within the range of 31−56% when compared with neat S-PLCL membranes, while in the solvent-cast film (F-PLCL), no significant change in WVP was observed. Moreover, S-PLCL demonstrated a controllable slow release rate of TC. S-PLCL loaded with 1500 μg/mL of TC showed a release concentration of 30 ppm over a certain time period to promote greater levels of human oral fibroblast and human oral keratinocyte cell proliferation and plaque inhibition. In conclusion, a TC-loaded S-PLCL fibrous membrane has been designed and fabricated to provide the ideal conditions for cell proliferation and antibiotic activity during treatment, outperforming nonfibrous F-PLCL loaded with TC at the same concentration.

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“…Nowadays, absorbable sutures in the market are Dexon (polyglycolic acid), Vicryl (polyglactin 910), Monocryl (polyglecaprone 25), Maxon (polyglyconate), PDS II (PDO, polydioxanone), Surgisorb M (PLC, 75/25 poly( l -lactide- co -ε-caprolactone)), and MonoMax (PHB, poly-4-hydroxybutyrate), which possess 40–60, 60–90, 90–120, 180, 200, and 400 days of complete absorption, respectively. − Among these sutures, PDO, PLC, and PHB are classified as long-term absorbable monofilament sutures. Although PLC copolymers (Figure ) have been reported in the literature for use in a wide range of biomedical applications such as drug delivery systems, barrier membranes, absorbable nerve guides, bioadhesives, and scaffolds for tissue engineering, relatively little attention has been paid to absorbable sutures, possibly because of their slow rate of absorption (typically > 12 months) in the human body. − However, for use with the specific tissues or organs such as tendon and ligament repairs or abdominal wall closure, where slow absorption is required, PLC copolymers have been shown to be effective and versatile materials that can be tailored through their composition to meet the specific property requirements of a given application. − …”

Section: Introductionmentioning

confidence: 99%

Development of an Antimicrobial-Coated Absorbable Monofilament Suture from a Medical-Grade Poly(l-lactide-co-ε-caprolactone) Copolymer

et al. 2021

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In this study, a medical-grade poly(l-lactide-co-ε-caprolactone) (PLC) copolymer with a monomer ratio of l-lactide (L) to ε-caprolactone (C) of 70:30 mol % for use as an absorbable surgical suture was synthesized via ring-opening polymerization (ROP) using a novel soluble liquid tin(II) n-butoxide (Sn(OnC4H9)2) as an initiator. In fiber fabrication, the process included copolymer melt extrusion with a minimal draw followed by sequential controlled hot-drawing and fixed-annealing steps to obtain oriented semicrystalline fibers with improved mechanical strength. For healing enhancement, the fiber was dip-coated with “levofloxacin” by adding the drug into a solution mixture of acetone, poly(ε-caprolactone) (PCL), and calcium stearate (CaSt) in the ratio of acetone/PCL/CaSt = 100:1% w/v:0.1% w/v. The tensile strength of the coated fiber was found to be increased to ∼400 MPa, which is comparable with that of commercial polydioxanone (PDS II) of a similar size. Finally, the efficiency of the drug-coated fiber regarding its controlled drug release and antimicrobial activity was investigated, and the results showed that the coated fiber was able to release the drug continuously for as long as 30 days. For fiber antimicrobial activity, it was found that a concentration of 1 mg/mL was sufficient to inhibit the growth of Staphylococcus aureus (MRSA), Escherichia coli O157:H7, and Pseudomonas aeruginosa, giving a clear inhibition zone range of 20–24 mm for 90 days. Cytotoxicity testing of the drug-coated fibers showed a %viability of more than 70%, indicating that they were nontoxic.

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Effect of topology of poly(L-lactide-co-ε-caprolactone) scaffolds on the response of cultured human umbilical cord Wharton’s jelly-derived mesenchymal stem cells and neuroblastoma cell lines

Cited by 9 publications

References 37 publications

Biocompatibility Assessment of PLCL‐Sericin Copolymer Membranes Using Wharton’s Jelly Mesenchymal Stem Cells

Biocompatibility Assessment of PLCL‐Sericin Copolymer Membranes Using Wharton’s Jelly Mesenchymal Stem Cells

Tetracycline-Loaded Electrospun Poly(l-lactide-co-ε-caprolactone) Membranes for One-Step Periodontal Treatment

Development of an Antimicrobial-Coated Absorbable Monofilament Suture from a Medical-Grade Poly(l-lactide-co-ε-caprolactone) Copolymer

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