Effect of topical ozonated sunflower oil on second intention wound healing in turtles: a randomised experimental study

Abstract

Background Ozone is an antimicrobial agent that in experimental and case-control studies has been found to exert a positive effect on wound healing. Wild and pet chelonians frequently present insidious wounds exhibiting secondary infections and/or delayed healing. Objectives Evaluate the effects of topical ozonated sunflower oil on second-intention healing of acute experimental skin wounds in red-eared sliders ( Trachemys scripta elegans ). Methods Randomised within-subject controlled study; Group 1 (n = 24) was used to assess clinical healing features; Group 2 (n = 12) was used for histological evaluation in which two sets of wounds were biopsied at 2, 7, 14, 21, 28 and 42 days over the course of the cicatrisation process. A single 6 mm diameter wound was made on each rear limb and topical ozonated (950 peroxide value) and non-ozonated sunflower oil were applied daily for one week on treated and contralateral control wounds, respectively. Results Mean wound size was significantly lower in the ozone-treated group at day 28 ( p < 0.0001) with differences of clinical relevance (74.04% vs. 93.05% reduction of initial wound size). Histologically, the acute inflammatory reaction was enhanced in treated wounds, with significantly higher numbers of heterophils ( p = 0.0016), lymphocytes ( p < 0.001) and fibroblasts ( p < 0.001). Conclusions Daily topical application of ozonated sunflower oil over the course of one week improved the healing of acute, full-thickness skin wounds in chelonians. This clinical outcome was histologically correlated with an enhanced acute inflammatory reaction, as well as the production and remodelling of collagen fibres.

Ozonated Sunflower Oil Exerted Protective Effect for Embryo and Cell Survival via Potent Reduction Power and Antioxidant Activity in HDL with Strong Antimicrobial Activity

“…Despite the many beneficial effects of OSO, it is unclear why OSO exerted favorable physiological effects on tissue regeneration and wound healing [ 14 ]. Tissue regeneration activity should be accompanied by sufficient antioxidant activity, such as the removal of radical superoxide and electron donation.…”

Ozonized water used as complementary therapy for stomatitis in Salvator merianae – case report

“…The results showed an (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v10i11.19550 increase in the presence of leukocyte cells, fibroblast proliferation, and early collagen fibre remodeling compared to the control group. Although the testudines do not have an effective capacity to contract wounds in centripetal motion, due to the absence of cutaneous muscles, wounds treated with ozone had a statistically greater decrease in diameter than those not treated (Ginel et al, 2021). Similar studies and case reports involving humans and other mammals are recurrent; however, in reptiles, they are still scarce (Fitzpatrick et al, 2018;Sciorsci et al, 2020;Ginel et al, 2021).…”

“…Although the testudines do not have an effective capacity to contract wounds in centripetal motion, due to the absence of cutaneous muscles, wounds treated with ozone had a statistically greater decrease in diameter than those not treated (Ginel et al, 2021). Similar studies and case reports involving humans and other mammals are recurrent; however, in reptiles, they are still scarce (Fitzpatrick et al, 2018;Sciorsci et al, 2020;Ginel et al, 2021).…”

“…In a recent experimental study, which was the first to evaluate ozone therapy in the healing of dermal lesions in reptiles, ozonized sunflower oil was shown to be effective in increasing the inflammatory response and formation of granulation tissue in experimentally induced wounds in Trachemys scripta elegans (Ginel et al, 2021). The results showed an (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v10i11.19550 increase in the presence of leukocyte cells, fibroblast proliferation, and early collagen fibre remodeling compared to the control group.…”

Ozonated Sunflower Oil Exerted Potent Anti-Inflammatory Activities with Enhanced Wound Healing and Tissue Regeneration Abilities against Acute Toxicity of Carboxymethyllysine in Zebrafish with Improved Blood Lipid Profile

“…Despite the several perks, OSO has not been probed meticulously for wound healing and tissue regeneration employing zebrafish, that considered the exemplary model for preclinical investigations [ 42 , 43 ]. Nonetheless, few preliminary observations on turtles substantiated the wound-healing role of OSO [ 44 ]. Pertaining to this, we delved into the comparative wound healing, tissue regenerative, and hepatoprotective efficiency of SO and OSO against CML-posed adversity in zebrafish.…”