Effect of toluene exposure on the antioxidant status and apoptotic pathway in organs of the rat

Kamel, Mostafa; Shehata, Mona

doi:10.1080/09674845.2008.11732801

Cited by 32 publications

(20 citation statements)

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“…They presented a significant increase in the caspase-3 activity, especially in the brain cortex [29]. It has been shown that toluene increases the caspase-3 activity by damaging the frontal cortex and brain stem [30].…”

Section: Discussionmentioning

confidence: 99%

Effects of Acute Toluene Toxicity on Different Regions of Rabbit Brain

Demır

Çiçek

Eser

et al. 2017

Analytical Cellular Pathology

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The acute phase effects of toluene on the brain have been investigated in this study using rabbit brain via histopathological, immunohistochemical, and biochemical methods. A total of 20 male rabbits were used as control and experimental groups. Moreover, nerve growth factor (NGF), tumor necrosis factor-alpha (TNF-alpha), dopamine (DA), and glial fibrillary acidic protein (GFAP) tests were performed in order to designate the severity of the biochemical damage. In the biochemical evaluation of the prefrontal cortex, hippocampus, hypothalamus, substantia nigra, and entorhinal cortex, the TNF-alpha levels in the brain were found to be significantly higher than in the control group. Levels of dopamine, secreted from the substantia nigra, nerve growth factor (NGF) developed from the hippocampal neurons, and GFAP, secreted from astrocyte cells, were detected to be significantly lower in the toluene-administration group than in the control group (p < 0.05). In addition, areas of focal vacuolar degeneration (abscess formation), gliosis, and perivascular demyelination, many pyknotic cells and necrosis were observed. In the toluene-administration group compared to the control group, distinct excessive expansions of the blood vessels and severe degeneration in the structure of cells and also dispersed cell borders were observed. Furthermore, abnormal malformations of the nuclei structure of the oligodendrocyte cells were seen. Bodies of the sequential neurons of the hippocampus in the toluene-administration group were distinctly structurally damaged compared to the control group. In addition, cytoplasm of the cortex cell showed serious immune reactivity in the experimental group.

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Section: Discussionmentioning

confidence: 99%

Effects of Acute Toluene Toxicity on Different Regions of Rabbit Brain

Demır

Çiçek

Eser

et al. 2017

Analytical Cellular Pathology

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“…MDA is generally recognised as a responsive biomarker of lipid peroxidation, according to Oboh et al (2012), and is considered a valuable indicator of oxidative stress status. Kamel et al (2008) reported significant increase in serum MDA among rats received 650mg /kg toluene daily for 15, 30 and 45 days.…”

Section: V Discussionmentioning

confidence: 89%

“…After 7 days, light microscopic analysis of immunohistochemically stained portions of the liver of the toluene group revealed strong positive caspase-3 immunoreactivity in the cytoplasm of hepatocytes, and weak positive caspase-3 immunoreactivity in the cytoplasm of hepatocytes of the toluene + pasturalized buffalo milk group revealed weak positive caspase-3 immunoreactivity in the cytoplasm of hepatocytes, compared to the negative control. In a research conducted by Kamel et al (2008), oral administration of 650 mg/kg toluene resulted in an increase in Caspase 3 activity in rat liver tissues.…”

Section: V Discussionmentioning

confidence: 99%

Evaluation of The Role of Buffalo Milk on Acute Toluene Induced Hepatotoxicity and Nephrotoxicity in Adult Male Albino Rats

Khalil

Hassaneine

El-Sheikh³

et al. 2021

Zagazig Journal of Forensic Medicine

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Background: Toluene is a volatile organic compound that is commonly used in a variety of applications around the world, including paints, inks, solvents, varnishes, plastics, thinners, fabrics, and dyes. This enhances the probability of exposure in occupational and environmental settings. Aim of the work: This research was carried out in adult male albino rats to look into the hepatic and renal histopathological changes caused by acute toluene exposure, the possible underlying mechanisms, and the role of buffalo milk in reducing the toxic effects of toluene. Methodology: The study was conducted for 7 days on seventy-two adult male albino rats. They were divided into five groups as follow: Group I (negative control): 12 rats were fed with regular diet and water to test basic parameters .Group II (positive control) 24 rats subdivided into 2 equal groups: Subgroup IIa (corn oil group)12 rats received 1 ml of corn oil (vehicle of toluene) by oral gavage . Subgroup IIb (distilled water group) 12 rats received 1ml of distilled water by oral gavage. Group III (pasturalized buffalo milk group -PBM) 12 rats received 1ml of buffalo milk by oral gavage. Group IV (toluene group) 12 rats received toluene 900mg/kg dissolved in corn oil by oral gavage. Group V (toluene and pasturalized buffalo milk group) 12 rats received toluene 900mg/kg followed by 1ml of buffalo milk by oral gavage for 7 days. At the end of the study, blood samples were collected for estimating (serum ALP, ALT, serum albumin, serum creatinine, serum MDA, TAC). Then livers and kidneys were extracted for histopathological (H&E) and immunohistochemistry (caspase-3) examination. Results: The result of the study revealed that administration of toluene for 7 days induced significant increase in ALP, ALT, creatinine, serum MDA and significant reduction in serum albumin and TAC. It also induced histopathological alterations in liver and kidney with increased caspase-3 immunoreactivity. Administration of PBM with toluene for 7 days improved toluene induced histopathological alterations in liver and kidney with decreased caspase-3 immunoreactivity. Also, PBM caused significantly beneficial effect on liver and kidney parameters; In addition, it decreased serum MDA level and increased serum TAC. Conclusion: Buffalo milk has partial protective effects against toluene induced hepatotoxicity, nephrotoxicity, oxidative stress and apoptosis.

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“…Other studies suggested that the reduction in glutathione in some tissues was caused by the effects of toluene exposure 19 . The results of research by Ahmadizadeh et al (2014) there was a significant difference in the reduction in glutathione level between the groups exposed to toluene and those that are not.…”

Section: Discussionmentioning

confidence: 99%

Correlation of Toluene Safe Concentration, Malondialdehyde and Glutathione Levels in Osowilangun Shoe Home Industry Workers

Rachma

Tualeka

Jalaludin

et al. 2020

IJFMT

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Introduction: Toluene is the main organic solvent found in adhesives used for shoe production in Osowilangun Shoes Home Industry. Exposure to toluene that exceeds the threshold value can cause the emergence of free radicals which eventually increase the concentration of malondialdehyde and reduce the concentration of glutathione in the body. The purpose of this research is to study the safe concentration of toluene with levels of malondialdehyde and glutathione in Osowilangun shoe home industry workers.Methods: This is an observational study using cross sectional design and quantitative approaches. There are 25 people were taken as sample by using accidental sampling technique. Data analysis used Pearson correlation test. Result:The average safe concentration value in this study was 1.1088 mg / m3 with an average value of malondialdehyde of 7,784 MU and glutathione of 35,655 μg / L. Pearson correlation test showed the safe concentration of toluene and malondialdehyde level was p=0.685 (p> 0.05), with relationship between safe concentrations of toluene and glutathione was p = 0.808 (p <0.05). Conclusion:This study shows that there was no significant relationship between toluene safe concentration and levels of malondialdehyde and glutathione.

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Effect of toluene exposure on the antioxidant status and apoptotic pathway in organs of the rat

Cited by 32 publications

References 0 publications

Effects of Acute Toluene Toxicity on Different Regions of Rabbit Brain

Effects of Acute Toluene Toxicity on Different Regions of Rabbit Brain

Evaluation of The Role of Buffalo Milk on Acute Toluene Induced Hepatotoxicity and Nephrotoxicity in Adult Male Albino Rats

Correlation of Toluene Safe Concentration, Malondialdehyde and Glutathione Levels in Osowilangun Shoe Home Industry Workers

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