Rabi season of 2016-17 to study the performance of barley varieties under various nitrogen levels in sodic soil. The trial was laid out in Split Plot Design. Nitrogen levels (60, 75 and 90 kg N ha -1 ) were kept in main-plots and varieties (RD-2907, RD-2552, NDB-1173 and RD-2794 in sub-plots with three replications on sodic soils having low organic carbon (0.38 g kg -1 ), available nitrogen (185.0 kg ha -1 ), phosphorus (15.25 kg ha -1 ) and potassium (265 kg ha -1 ). Data manifested to nitrogen content and uptake of barley grain and straw as influenced by nitrogen levels and varieties. Maximum nitrogen content (1.68) in grain, (0.54%) in straw determined with application of 90 kg N ha -1 which was at par with the dose of 75 kg N ha -1 and lowest with 60 kg N ha -1 . The Maximum nitrogen uptake in grain (47.13 kg ha -1 ) and straw (24.80 kg ha -1 ) was recorded under the supply of 90 kg N ha -1 which was significantly higher than 75 and 60 kg N ha -1 . There was no significant difference in term of nitrogen content in grain due to different varieties of barley crop. Maximum nitrogen content (0.55) in straw was recorded in variety RD-2907 which was at par with RD-2552 (0.54%) and significantly maximum over rest of the varieties. Among the varieties of barley, maximum nitrogen uptake by grain and straw (48.31 and 25.25 kg ha -1 , respectively) was recorded in variety (RD-2907) which was at par with (24.52) RD-2552 and significantly superior to RD-2794 and NDB-1173. Nitrogen and protein content in grain increased with increasing levels of nitrogen. Maximum nitrogen content (1.68%) and protein content (10.54%) in grain was observed with 90 kg N ha -1 . The varieties had no significant effect on nitrogen and protein content in grain.