Effect of TiF4/NaF and chitosan solutions on the development of enamel caries under a microcosm biofilm model

Vertuan, Mariele; Machado, Paula Fontana; Souza, Beatriz Martines de; Braga, Aline Silva; Magalhães, Ana Carolina

doi:10.1016/j.jdent.2021.103732

Cited by 6 publications

(1 citation statement)

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“…In order to intuitively observe the damage of biofilm, we used an optimized biofilm model to observe its integrity. Frequently, to simulate the progression of dental caries, materials such as hydroxyapatite disks, bovine dentin disks or bovine teeth are used by researchers to construct dental caries biofilms ( Ribeiro-Vidal et al., 2020 ; Neves et al., 2019 ; Vertuan et al., 2021 ). The biofilms formed in these models are relatively loose due to the hydrophobicity of material ( Cui et al., 2018 ), and would not be suitable for analyzing the effects of ANEOs on cell arrangement in the biofilm.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial mechanism of areca nut essential oils against Streptococcus mutans by targeting the biofilm and the cell membrane

Liu,

Zhang,

et al. 2023

Front. Cell. Infect. Microbiol.

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IntroductionDental caries is one of the most common and costly biofilm-dependent oral diseases in the world. Streptococcus mutans is the major cariogenic pathogen of dental caries. S. mutans synthesizes extracellular polysaccharides by autologous glucosyltransferases, which then promotes bacterial adhesion and cariogenic biofilm formation. The S. mutans biofilm is the principal target for caries treatment. This study was designed to explore the antibacterial activity and mechanisms of areca nut essential oil (ANEO) against S. mutans.MethodsThe ANEOs were separated by negative pressure hydro-distillation. The Kirby-Bauer method and broth microdilution method were carried out to evaluate the antibacterial activity of different ANEOs. The antibacterial mechanism was revealed by crystal violet staining, XTT reduction, microbial adhesion to hydrocarbon test, extracellular polysaccharide production assay, glucosyltransferase activity assay, lactate dehydrogenase leaking, propidium iodide staining and scanning electron microscopy (SEM). The cytotoxicity of ANEOs was determine by MTT assay.ResultsThe ANEOs separated at different temperatures exhibited different levels of antibacterial activity against S. mutans, and the ANEO separated at 70°C showed the most prominent bacteriostatic activity. Anti-biofilm experiments showed that the ANEOs attenuated the adhesion ability of S. mutans by decreasing the surface hydrophobicity of the bacteria, prevented S. mutans biofilm formation by inhibiting glucosyltransferase activity, reducing extracellular polysaccharide synthesis, and reducing the total biofilm biomass and activity. SEM further demonstrated the destructive effects of the ANEOs on the S. mutans biofilm. Cell membrane-related experiments indicated that the ANEOs destroyed the integrity of the cell membrane, resulting in the leakage of lactic dehydrogenase and nucleic acids. SEM imaging of S. mutans cell showed the disruption of the cellular morphology by the ANEOs. The cytotoxicity assay suggested that ANEO was non-toxic towards normal oral epithelial cells.DiscussionThis study displayed that ANEOs exerted antibacterial activity against S. mutans primarily by affecting the biofilm and disrupting the integrity of the cell membrane. ANEOs has the potential to be developed as an antibacterial agent for preventing dental caries. Additionally, a new method for the separation of essential oil components is presented.

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