Effect of the Various Solvents on the &lt;i&gt;In Vitro&lt;/i&gt; Permeability of Vitamin B&lt;sub&gt;12&lt;/sub&gt; through Excised Rat Skin

Moghimipour, Eskandar; Salimi, Anayatollah; Makhmalzadeh, Behzad Sharif

doi:10.4314/tjpr.v12i5.2

Cited by 16 publications

(15 citation statements)

References 15 publications

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“…2 Vaddi et al were obtained three endothermic transition at 62, 79, and 95°C in the thermotropic behavior of rat skin 21 while Shakeel et al 24 observed four endothermic transitions at 34, 82, 105, and 114°C. Kaushik et al reported in human dermal DSC graphs and observed three endothermic transition peaks at temperatures 59-63°C (Tm 1 ), 75-82°C (Tm 2 ) and 99.5-120°C (Tm 3 ).…”

Section: Naproxen Permeabilitymentioning

confidence: 97%

“…1 Avoiding first pass metabolism , sustained and controlled delivery of drugs, decrease of dose frequency, proper patient compliance and localization of drugs in target site, avoidance of the risks of injections, and reduction in toxic level of drugs are among TDD advantages. [2][3][4][5][6] Skin permeation of drugs is an essential stage percutaneous absorption. Permeation is the penetration from one layer into another layer of skin.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The Effect of Various Vehicles on the Naproxen Permeability through Rat Skin: A Mechanistic Study by DSC and FT-IR Techniques

Salimi¹,

Hedayatipour²,

Moghimipour³

2016

Adv Pharm Bull

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Section: Naproxen Permeabilitymentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

The Effect of Various Vehicles on the Naproxen Permeability through Rat Skin: A Mechanistic Study by DSC and FT-IR Techniques

Salimi¹,

Hedayatipour²,

Moghimipour³

2016

Adv Pharm Bull

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“…On the other hand, lipid groups are oriented again, a phenomenon that causes a shift to lower wave number (e.g., redshift) and strengthening of subcutaneous barrier properties which finally slows down the entrance of permeant through the skin [11]. If the penetration modifier performs by affecting on lipid pathway, the phase transition of the lipids is showed by increase/decrease in the band position (wavenumber) of the signals at 2920, 2850, and near 1738 cm −1 [12]. Thermal analysis methods such as DSC have been utilized to investigate thermal transitions in mammalian SC.…”

Section: Saadatzadeh Et Almentioning

confidence: 99%

“…1. Table 1 shows the effect of enhancers on ketorolac permeability as ER flux (ratio of drug flux before and after exposure to enhancer) and ER D (drug diffusion before and after exposure to enhancer) in comparison with control [12].…”

Section: Effect Of Enhancers On Ketorolac Skin Permeabilitymentioning

confidence: 99%

Influence of Permeation Enhancers on the in Vitro Skin Permeation of Ketorolac Tromethamine Through Excised Rat Skin: A Mechanistic Study

Saadatzadeh

Salimi

Zarooni

2018

Asian J Pharm Clin Res

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Objective: Ketorolac tromethamine (KT) is considered as a member of Non-steroidal anti-inflammatory drugs that used as an analgesic and antipyretic agent. The main aim of this research was to investigate the effect of some herbal and chemical permeation enhancers on the in vitro skin permeability of KT.Method: Ketorolac permeability experiments through rat skin pre-treated with some of permeation enhancers, namely urea, eucalyptus oil, olive oil, and menthol, were performed in fabricated Franz diffusion cells and compared with hydrated rat skin as control. The permeability parameters evaluated include steady-state flux (Jss), permeability coefficient (Kp), and diffusion coefficient (D). The penetration enhancers permeability enhancement mechanisms were investigated by comparing changes in peak position and their intensities of asymmetric (Asy) and symmetric (Sym) C-H stretching, C=O stretching, C=O stretching (Amide I), and C-N stretching of keratin (Amide II) absorbance using fourier transform infrared spectroscopy (FT-IR), as well as by comparing mean transition temperature (Tm) and their enthalpies (ΔH) using differential scanning calorimetery (DSC). KT permeability parameters through rat skin were evaluated with and without chemical enhancers such as eucalyptus oil, olive oil, menthol, and urea.Result: The skin showed barrier for ketorolac permeability through the whole skin and that diffusion into the skin was the rate-limiting step for drug flux. Urea, eucalyptus oil, olive oil, and menthol were the most effective enhancers as they increased flux 0.02, 0.017, 0.016, and 0.012 times and diffusion coefficient 0.079, 0.194, 0.129, and 1.35 folds in comparison with hydrated skin, respectively.Conclusion: FT-IR and DSC results showed lipid fluidization, extraction, disruption of lipid structure, and irreversible denaturation of proteins in the stratum corneum layer of the skin by permeation enhancers.

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“…The animals were treated according to the principles for the care, and use of laboratory animals and the procedures followed with the standard international guidelines. [8] …”

Section: Animal Studiesmentioning

confidence: 99%

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Salimi¹

2017

AJP

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Introduction: Hydroquinone (HQ), a famous anti-hyperpigmentation agent, suffers from several weaknesses such as instability due to rapid oxidation and insufficient skin penetration because of the hydrophilic structure. The aim of this research was to formulate, characterize and evaluation of in vitro skin permeability of HQ-loaded microemulsion (ME). Methods: HQ MEs were prepared by pseudoternary phase diagram method, the appropriate ratios of oil, s/c mixture, and water were chosen, and full factorial design was used with three variables at two levels for preparing eight formulations. The prepared MEs were evaluated regarding their droplet size, viscosity, pH, differential scanning calorimetry, stability, in vitro drug release, and in vitro skin permeability. Results and Discussion:The results showed that the mean droplet size range of ME samples was in the range of 7.05-79.56 nm and pH was 5.3-5.7, respectively. Viscosity range of MEs was 109-195 cps. Drug release profile showed that 90.51% of the drug released (ME-HQ-8) in the 24 h of the experiment. The kinetics of drug release from all selected MEs were approximately described by Higuchi model and showed prolonged release when compared to HQ solution. All ME formulations with different compositions and properties significantly increased flux and permeability coefficient from rat skin. J ss and Papp parameters in ME-HQ-2 formulation were 0.404 mg/cm 2 h, 0.02 cm/h, and 4 times higher than those of control, respectively. The selective MEs have 99.9% HQ amount after 6 months storage. They have visually cleared and no any color changes, thus HQ MEs can could been protect drug for a long time without antioxidant. Conclusion: The present research established that the amount of components of water, oil, and S + C in ME formulation plays an important role in the physicochemical properties and permeability parameters. This study showed that any change in content and composition of MEs could be changed physicochemical properties and permeability parameters during drug permeation from ME samples. The studied MEs increased permeation rate and permeability coefficient through rat skin. Our results were showed that ME formulation could not be increase diffusivity of HQ in stratum corneum.

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Effect of the Various Solvents on the <i>In Vitro</i> Permeability of Vitamin B<sub>12</sub> through Excised Rat Skin

Cited by 16 publications

References 15 publications

The Effect of Various Vehicles on the Naproxen Permeability through Rat Skin: A Mechanistic Study by DSC and FT-IR Techniques

The Effect of Various Vehicles on the Naproxen Permeability through Rat Skin: A Mechanistic Study by DSC and FT-IR Techniques

Influence of Permeation Enhancers on the in Vitro Skin Permeation of Ketorolac Tromethamine Through Excised Rat Skin: A Mechanistic Study

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