Effect of the Degree of Hyperglycaemia oft the Catalytic Activities of Glycosidases in Kidney and Urine of Diabetic Rats

Abstract

Juretio, Lipovac, Hadzija and Slijepcevio: Glycosidases-hyperglycaemia relationships in rat kidney (urine) 21 Summary: The catalytic activities of N-acetyl-ß-D-glucosaminidase % , ß-galactosidase and -glucosidase in kidney and urine of diabetic rats were investigated in relation to the duration of diabetes, to the degree of constant hyperglycaemia and to the therapeutic control in the early stage of disease.The results suggest that the degree of constant hyperglycaemia and the duration of untreated diabetes are significant determining factors for the course of morphological changes. These changes are manifested äs a decrease of the glycosidases in kidney (0.5 to 0.6 time the age-matched controls) and äs moderate to severe enzymurias.Daily Variation of blood glucose with inadequate insulin Lente therapy caused decreased N-acetyl-ß-Dglucosaminidase and ß-galactosidase activities in kidney äs well äs enzymuria. Since such changes can be correlated with histologically visible changes in the kidney, the measurement of these enzymes in urine is a simple way of monitoring the development of kidney damage in poorly controlled diabetes.When constant normöglycaemia was maintained for three weeks with insulin Ultralente in diabetic rats with a confirmed decrease of kidney glycosidases, the persisting morphological alteration of the organ was reflected by a urinary Output of N^acetyl-ß-D-glucosaminidase. Einfluß des Hyperglykämie-Grades auf die katalytischen Aktivitäten von Glykosidasen in Nieren und Harn diabetischer RattenZusammenfassung: Die katalytisehen Aktivitäten von N-Acetyl-ß-D-glucosaminidase, ß-Galaktosidase und -Glucosidase in Niere und Harn diabetischer Ratten wurden untersucht und in Beziehung gesetzt zur Dauer des Diabetes, zum Grad einer konstanten Hyperglykämie und zur therapeutischen Kontrolle im Früh-stadium der Erkrankung.Die Ergebnisse weisen darauf hin, daß der Grad konstanter Hyperglykämie und die Dauer eines unbehandelten Diabetes bedeutende Einflußgrößen für den Ablauf morphologischer Veränderungen sind. Die Verän-derungen werden manifest als Abfall der Glykosidasen in der Niere auf das 0,5-0,6-fache gleichaltriger Kontrollen und als mäßige bis starke Enzymurien.Tägliche Änderung der Glucosekonzentration im Blut durch inadäquate Therapie mit Insulin Lente bewirkte erniedrigte katalytische Aktivitäten von N-Acetyl-ß-D-glucosaminidase und ß-Galaktosidase in der Niere als auch Enzymurie. Da solche Änderungen mit histologisch sichtbaren in der Niere korreliert werden können, ist die Bestimmung dieser Enzyme im Harn ein einfacher Weg, die Entwicklung eines Nierenschadens bei schlecht kontrolliertem Diabetes zu verfolgen. J. Clin. Chem. Clin. Biochem. / Vol. 22,1984 / No. l Brought to you by | University of Arizona Authenticated Download Date | 7/11/15 1:17 AM 22Jurctic, Lipovac, Hadzija and Slijepcevio: Glycosidases-hyperglycaemia relationships in rat kidney (urine) Wenn bei diabetischen Ratten mit einem bestätigtem Abfall der Glykosidasen in der Niere für 3 Wochen eine konstante Normoglykämie durch I...

Modulatory Effect of Fenugreek Seed Mucilage and Spent Turmeric on Intestinal and Renal Disaccharidases in Streptozotocin Induced Diabetic Rats

“…A decrease in renal disaccharidases activity was observed during diabetes [30][31][32][33]. The renal maltase activity in diabetic rats was significantly lower than that in control rats (Table 4).…”

EFFECT OF BANANA (MUSASP. CULTIVAR ELAKKI BALE) FLOWER AND STEM ON ENZYME ACTIVITIES OF INTESTINAL AND RENAL DISACCHARIDASES IN STREPTOZOTOCIN-INDUCED DIABETIC RATS

“…1972). Decrease in renal disaccharidase activity was observed during diabetes (Juretic et al. 1984; Sharma and Sivakami 1984; Iizuka et al.…”

“…The presence of disaccharidases activity in subcellular fractions of the renal cortex is probably due to its involvement in the digestion or transport of sugars across the membranes (Taylor et al 1972). Decrease in renal disaccharidase activity was observed during diabetes (Juretic et al 1984;Sharma and Sivakami 1984;Iizuka et al 1989). The role of renal disaccharidases though is not extensively studied, but there are quite a few reports that deal with these renal enzyme activities.…”