Effect of the combination of metformin hydrochloride and melatonin on oxidative stress before and during pregnancy, and biochemical and histopathological analysis of the livers of rats after treatment for polycystic ovary syndrome

Lemos-Jordão, Ana Janaina Jeanine Martins de; Peixoto, Christina A.; Teixeira, Álvaro Aguiar Coelho; Luna, Rayana Leal de Almeida; Rocha, Sura Wanessa Santos; Santos, Hilda Michelly Paiva dos; Silva, Amanda Karolina S.; Nunes, Ana Karolina Santana; Wanderley‐Teixeira, Valéria

doi:10.1016/j.taap.2014.05.015

Cited by 24 publications

(12 citation statements)

References 45 publications

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“…The 4 μ m sections were obtained in a microtome, model RM 2245 (Leica, Wetzlar, Germany) and subject to staining techniques by hematoxylin and eosin (H&E). The slides in duplicate/animal were examined under a light microscope and photographed using Leica camera, model EC3, attached to the microscope and the Leica Application Suite software EZ (Leica, Wetzlar, Germany) used for histopathology [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L.

Brito

Almeida

Santana

et al. 2018

Oxidative Medicine and Cellular Longevity

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Spondias mombin L. is used in folk medicine for the treatment of inflammation and gastrointestinal diseases. Our study investigated the antiulcer activity of S. mombin ethanolic extract (SmEE) and its majority compounds gallic acid (GA) and ellagic acid (EA). Phytochemical characterization was performed by HPLC. The SmEE was screened for in vitro antioxidant activities using phosphomolybdenum, ABTS, DPPH, and FRAP assays. The antiulcer activity of SmEE, GA, EA, or GA + EA was evaluated by gastric lesion models induced by absolute ethanol and indomethacin. Following this, it is capable of stimulating mucus production, antisecretory capacity, and the influence of −SH groups and NO in the effect of SmEE. Its healing activity was demonstrated by acetic acid-induced chronic ulcer model. Anti-Helicobacter pylori activity was assessed by determining the MIC of the SmEE (64–1024 μg/mL). The HPLC results identified the presence of gallic acid and ellagic acid in SmEE. The extract showed antioxidant activity in vitro. SmEE (50, 100, and 200 mg/kg) reduced the area of ulcerative lesions induced by ethanol in 23.8, 90.3, and 90.2%, respectively. In NSAID model, the SmEE induced protection of 36.8, 49.4, and 49.9%, respectively. GA (10 mg/kg) or EA (7 mg/kg) or the association of GA + EA (10 + 7 mg/kg) inhibited the ethanol-induced lesions in 71.8, 70.9, and 94.9%, respectively, indicating synergistic action. SmEE (100 mg/kg) decreased acid secretion and H+ concentration in the gastric contents, increased levels of mucus, and showed to be dependent of −SH groups and NO on the protection of the gastric mucosa. In chronic ulcer model, SmEE reduced the gastric area lesion. SmEE showed anti-H. pylori activity. In conclusion, our study showed that SmEE has antiulcerogenic activity. GA and EA are isolated gastric protectors and, when associated, acted synergistically to protect the gastric mucosa.

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Section: Methodsmentioning

confidence: 99%

Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L.

Brito

Almeida

Santana

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…Ultrathin sections (4 μ m) were obtained using a microtome (model RM 2245, Leica Biosystems GmbH, Nussloch, Germany) and subjected to hematoxylin and eosin (HE) staining. Two slides were prepared for each animal, and all of them were examined under a light microscope (model DM500, Leica Biosystems GmbH, Nussloch, Germany), photographed using Leica camera (model EC3, Leica Biosystems GmbH, Nussloch, Germany) attached to the microscope, and then histopathologically analyzed using Leica Application Suite (LAS) EZ microscope software (Leica Biosystems GmbH, Nussloch, Germany) [ 43 , 44 ].…”

Section: Methodsmentioning

confidence: 99%

Spondias purpurea L. (Anacardiaceae): Antioxidant and Antiulcer Activities of the Leaf Hexane Extract

Almeida

Brito

Santana

et al. 2017

Oxidative Medicine and Cellular Longevity

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Spondias purpurea is used in folk medicine to treat diarrhea and diuresis. The objective of this study was to evaluate the phytochemical profile and antioxidant and antiulcer activities of the hexane extract of the leaves of S. purpurea (SpHE). Phytochemical profile was evaluated via thin layer chromatography (TLC) and HPLC. SpHE was screened for antioxidant activities using DPPH, ABTS, FRAP, and phosphomolybdenum assays. To determine its antiulcer properties, animals were pretreated with injured control, lansoprazole, ranitidine, carbenoxolone, or SpHE (12.5, 25, and 50 mg/kg) and were screened; acute ulcers were induced by HCl/ethanol, absolute ethanol, and nonsteroidal anti-inflammatory drug (NSAID). TLC revealed the presence of flavonoids, whereas HPLC analysis showed the presence of caffeic acid and epigallocatechin. The phenolic compounds and in vitro assays showed antioxidant activity. After gastric ulcer induction by using HCl/ethanol, SpHE reduced the area of ulcerative lesions by 82, 91, and 88%, respectively. In ethanol, SpHE reduced the area of ulcerative lesions by 77, 93, and 92%, respectively. In the NSAID, the percentages of protection were 70, 76, and 78%, respectively. SpHE promoted the minimization of ulcers, increased the levels of reduced glutathione, and decreased tumor necrosis factor. S. purpurea has antioxidant and antiulcer properties.

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“…113 The PCOS rats induced by constant illumination had an increase in the number and diameter of ovarian cysts, thickening of the tunica albuginea, a lack of primary and growing follicles, and numerous atretic follicles. 115 TNFα may induce oxidative stress and decreases melatonin levels in the follicle. 115 TNFα may induce oxidative stress and decreases melatonin levels in the follicle.…”

Section: Mel Atonin and P Olyc Ys Ti C Ovary Syndromementioning

confidence: 99%

“…114 They also presented degeneration and diffuse hyperplasia of the Kupffer cells in livers, as well as alterations in the plasmatic levels of cholesterol, triglycerides, enzymatic alterations, and alterations of the cytokines including iNOS, IL-1β, and TNFα. 115 TNFα may induce oxidative stress and decreases melatonin levels in the follicle. 116 Therefore, the organisms with PCOS have an unstable milieu with an increased oxidative stress.…”

Section: Mel Atonin and P Olyc Ys Ti C Ovary Syndromementioning

confidence: 99%

Pleiotropic roles of melatonin in endometriosis, recurrent spontaneous abortion, and polycystic ovary syndrome

Yang

Zhou

et al. 2018

American J Rep Immunol

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Melatonin is a neurohormone synthesized from the aromatic amino acid tryptophan mainly by the pineal gland of mammals. Melatonin acts as a broad-spectrum antioxidant, powerful free radical scavenger, anti-inflammatory agent, anticarcinogenic factor, sleep inducer and regulator of the circadian rhythm, and potential immunoregulator. Melatonin and reproductive system are interrelated under both physiological and pathological conditions. Oxidative stress, inflammation, and immune dysregulation are associated with the pathogenesis of the female reproductive system which causes endometriosis (EMS), recurrent spontaneous abortion (RSA), and polycystic ovary syndrome (PCOS). Accumulating studies have indicated that melatonin plays pleiotropic and essential roles in these obstetrical and gynecological disorders and would be a candidate therapeutic drug to regulate inflammation and immune function and protect special cells or organs. Here, we systematically review the pleiotropic roles of melatonin in EMS, RSA, and PCOS to explore its pathological implications and treatment potential.

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Effect of the combination of metformin hydrochloride and melatonin on oxidative stress before and during pregnancy, and biochemical and histopathological analysis of the livers of rats after treatment for polycystic ovary syndrome

Cited by 24 publications

References 45 publications

Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L.

Antiulcer Activity and Potential Mechanism of Action of the Leaves of Spondias mombin L.

Spondias purpurea L. (Anacardiaceae): Antioxidant and Antiulcer Activities of the Leaf Hexane Extract

Pleiotropic roles of melatonin in endometriosis, recurrent spontaneous abortion, and polycystic ovary syndrome

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