Effect of temporal variation, gender and size on cuticle polyphenol oxidase activity in deep-water rose shrimp (Parapenaeus longirostris)

Bono, G.; Badalucco, C.; Corrao, A.; Cusumano, S.; Mammina, Luigi Maria; Palmegiano, G. B.

doi:10.1016/j.foodchem.2010.04.055

Cited by 12 publications

(9 citation statements)

References 19 publications

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“…Sodium metabisulfite (1.25 and 2.5%) slowed down significantly the melanosis of shrimps (X. kroyeri), until 4-5 days, but thereafter no significant differences were shown with control shrimps, exhibiting all of them a severe degree of melanosis (Galvão et al, 2017). PPO is more active in autumn than in winter (Bono et al, 2010), which could explain the low effectiveness of the sulphite-based formulations at the concentration tested in the present work. Martínez-Alvarez et al, (2005a) noticed that dusting shrimp captured in autumn with the same concentration of commercial metabisulphite delayed the appearance of melanosis for at least 4 days.…”

Section: Resultsmentioning

confidence: 64%

Several melanosis-inhibiting formulas to enhance the quality of deepwater pink shrimp (Parapenaeus longirostris)

López‐Caballero

Martínez-Álvarez

Gómez‐Guillén

et al. 2019

Innovative Food Science & Emerging Technologies

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The aim of this work was to assess the effect of several melanosis-inhibiting formulations on quality of deepwater pink shrimp (Parapenaeus longirostris) during storage. The formulations tested were as follows: one containing 4-hexylresorcinol (0.1%) in combination with organic acids (citric, ascorbic and acetic) and chelating agents (ethylenediaminetetraacetic acid [EDTA] and di-sodium di-hydrogen pyrophosphate [PPi]); a commercial formula based on sulphites (3%), and another formulation containing a mixture of chitosan (0.3%) and commercial sulphites. Nontreated shrimp were used as control. The 4-hexylresorcinol-based formulation was more effective than 3% of commercial sulphite solutions in preventing melanosis. This treatment prolonged the sensory acceptability of shrimp by up to six days in ice storage.During chilled storage, the treatment with the 4-hexylresorcinol-based formula inhibited the growth of microorganisms by 1.5-2 log cycles as compared to commercial sulphites, especially H2S-producers and pseudomonads. At the end of the experimental period, TVB-N levels were slightly higher in the lot treated with 4-hexylresorcinol (33 mg/100 mg) and reflected greater endogenous enzymatic activity, since the counts of the spoiler microorganisms remained around log 5 cfu/g or even lower. In general, the incorporation of chitosan to sulphites did not improve or produce a marked effect on shrimps during storage. During the sensory analysis, the panellists rated all the lots very similar, although the 4-hexylresorcinol formulation was the only one with a neutral odour at the end of the period. The 4-hexylresorcinol-based formulation induced yellow-green colorations to some extent in the cephalothorax, possibly due to the effect of organic acids and chelants, while the rest of the lots presented greyish/blackish colorations. Therefore, the formulation based on 4-hexylresorcinol may safely substitute traditional sulphites to improve the quality of fresh shrimp and prolong their shelf life during storage.

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Section: Resultsmentioning

confidence: 64%

Several melanosis-inhibiting formulas to enhance the quality of deepwater pink shrimp (Parapenaeus longirostris)

López‐Caballero

Martínez-Álvarez

Gómez‐Guillén

et al. 2019

Innovative Food Science & Emerging Technologies

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“…Kinetika enzim ditentukan menggunakan metode Bono et al (2010). Sebanyak 0,2 mL enzim ditambahkan dengan 2,8 mL L-DOPA yang dilarutkan dalam 0,05 M buffer fosfat dengan pH sesuai pH optimum.…”

Section: Penentuan Kinetika Enzimunclassified

“…Pengaruh ion logam dan inhibitor terhadap enzim ditentukan menggunakan modifikasi metode Bono et al (2010 …”

Section: Pengaruh Ion Logam Dan Inhibitor Terhadap Enzimunclassified

CHARACTERIZATION OF CRUDE EXTRACT POLYPHENOLOXIDASE ENZYME FROM BLACK TIGER SHRIMP (Penaeus monodon)

Suhandana¹,

Nurhayati²,

Ambarsari³

2013

J. Ilmu dan Teknologi Kelautan Tropis

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Shrimp is a very important export commodity with high market value world wide. However, it is still facing problem related to the waste and deterioration quality as main issues for the shrimp industry. In this experiment, polyphenoloxidase from the carapace of Penaeus monodon was extracted and characterized. The research was carried out to obtain the optimum extraction condition and to evaluate the properties of enzyme i.e., pH, optimum temperature for activating enzyme, kinetic enzyme, and chelating on metal ion. The best method for PPO enzyme extraction used buffer with 1:3 proportion. The optimum activity of enzyme was at pH 7 and temperature of 35°C. The kinematic enzyme (Km) value and the maximum substrate concentration were 5.42 mM and 7.5 mM, respectively. Na+, Ca2+, Zn2+, and EDTA with concentration 5 and 10 mM inhibited enzyme activity. Cu2+at concentration of 10 mM and Mn2+ at concentration 5 mM also inhibited enzyme activity Keywords: carapace, characterization, polyphenoloxidase, shrimp

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“…Pengaruh ion logam dan inhibitor terhadap enzim ditentukan menggunakan modifikasi metode Bono et al (2010). Sampel enzim 0,2 mL ditambahkan dengan 0,2 mL larutan Cu 2+ , Ca 2+ , Zn 2+ , Mn 2+ , Na, Co 2+ , ethylene diamine tetraacetic acid (EDTA) dilarutkan dalam 20 mM buffer Tris-HCl (pH 7,1).…”

Section: Pengaruh Ion Logam Dan Inhibitor Terhadap Enzimunclassified

“…http://itk.fpik.ipb.ac.id/ej_itkt52 2.7. Pengujian Aktivitas Enzim (Bono et al, 2010) Aktivitas polyphenoloxidase ditentukan dengan mereaksikan 0,2 mL enzim ditambahkan dengan 2,8 mL 0,01 M L-DOPA yang dilarutkan dalam 0,05 M buffer fosfat pH 6,5. Campuran reaksi kemudian diinkubasi pada suhu 35 o C selama 5 menit.…”

Section: Pengaruh Ion Logam Dan Inhibitor Terhadap Enzimunclassified

CHARACTERIZATION OF CRUDE EXTRACT POLYPHENOLOXIDASE ENZYME FROM BLACK TIGER SHRIMP (Penaeus monodon)

Suhandana¹,

Nurhayati²,

Ambarsari³

2013

J. Ilmu dan Teknologi Kelautan Tropis

View full text Add to dashboard Cite

Shrimp is a very important export commodity with high market value world wide. However, it is still facing problem related to the waste and deterioration quality as main issues for the shrimp industry. In this experiment, polyphenoloxidase from the carapace of Penaeus monodon was extracted and characterized. The research was carried out to obtain the optimum extraction condition and to evaluate the properties of enzyme i.e., pH, optimum temperature for activating enzyme, kinetic enzyme, and chelating on metal ion. The best method for PPO enzyme extraction used buffer with 1:3 proportion. The optimum activity of enzyme was at pH 7 and temperature of 35°C. The kinematic enzyme (Km) value and the maximum substrate concentration were 5.42 mM and 7.5 mM, respectively. Na+, Ca2+, Zn2+, and EDTA with concentration 5 and 10 mM inhibited enzyme activity. Cu2+at concentration of 10 mM and Mn2+ at concentration 5 mM also inhibited enzyme activity Keywords: carapace, characterization, polyphenoloxidase, shrimp

show abstract

Effect of temporal variation, gender and size on cuticle polyphenol oxidase activity in deep-water rose shrimp (Parapenaeus longirostris)

Cited by 12 publications

References 19 publications

Several melanosis-inhibiting formulas to enhance the quality of deepwater pink shrimp (Parapenaeus longirostris)

Several melanosis-inhibiting formulas to enhance the quality of deepwater pink shrimp (Parapenaeus longirostris)

CHARACTERIZATION OF CRUDE EXTRACT POLYPHENOLOXIDASE ENZYME FROM BLACK TIGER SHRIMP (Penaeus monodon)

CHARACTERIZATION OF CRUDE EXTRACT POLYPHENOLOXIDASE ENZYME FROM BLACK TIGER SHRIMP (Penaeus monodon)

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