Effect of surfactants on cellulose hydrolysis

Helle, Steve S.; Duff, Sheldon J.B.; Cooper, David G.

doi:10.1002/bit.260420509

Cited by 250 publications

(156 citation statements)

References 27 publications

(2 reference statements)

Supporting

Mentioning

146

Contrasting

Unclassified

Order By: Relevance

“…Biosurfactants present higher environmental compatibility and higher activity at extreme temperature, pH and salinity [19,20] . Another important result reported by Helle [21] is that the surfactant effect is higher at low cellulase 5th International Conference on Civil, Architectural and Hydraulic Engineering (ICCAHE 2016) concentration. However, few researches have presented the effect of surfactants addition for hydrolysis of cellulose at relatively low enzyme dosage.…”

Section: Introductionmentioning

confidence: 94%

“…Other mechanisms include that surfactant is able to change the nature of the substrate, thereby increasing the availability of cellulose surface; in turn promoting more sites for cellulases to be adsorbed onto [21,24] . It is shown by the correlation between surfactant concentration and cellulose conversion that the higher concentration of surfactant does not always lead to the higher increment in cellulose conversion.…”

Section: Enzymatic Hydrolysismentioning

confidence: 99%

See 1 more Smart Citation

Hydrolysis of pretreated rice straw with surfactants at low cellulase dosage

Liang¹,

Huang²,

Miao³

et al. 2016

Proceedings of the 2016 5th International Conference on Civil, Architectural and Hydraulic Engineering (ICCAHE 2016)

View full text Add to dashboard Cite

Abstract:The effects of three surfactants (Tween 80, saponin and monorhamnolipid) on the hydrolysis of alkali pretreated rice straw by low dosage of cellulase were studied. The results indicated that with a relatively low cellulase dosage (4 FPU g -1 substrate), all surfactants were able to enhance the enzymatic hydrolysis, while the biosurfactant monorhamnolipid was demonstrated to be more active than Tween 80 and saponin. Monorhamnolipid at 0.006% increased reducing sugar yield by 23.15%, and the maximal sugar yield was obtained within half of the time compare to the control required. The cellulase losed 67% of its activity in the control, while the activity lost ratios were 31%~43% in the presence of the surfactants. The surfactants addition did not affect the pH of the hydrolysis system. This study provides an opportunity of decreasing enzyme dosage in hydrolysis of agricultural solid waste rich in cellulose.

show abstract

Section: Introductionmentioning

confidence: 94%

Section: Enzymatic Hydrolysismentioning

confidence: 99%

Hydrolysis of pretreated rice straw with surfactants at low cellulase dosage

Liang¹,

Huang²,

Miao³

et al. 2016

Proceedings of the 2016 5th International Conference on Civil, Architectural and Hydraulic Engineering (ICCAHE 2016)

View full text Add to dashboard Cite

show abstract

“…Surfactant could change the nature of the substrate, e.g., by increasing the available cellulose surface or by removing inhibitory lignin. Additionally, surfactant could increase the stability of the enzymes and thus reduce the amount of enzyme denaturation during hydrolysis, which is favorable to enzymatic hydrolysis (Helle et al 1993). Fig.…”

Section: Glucose Yieldsmentioning

confidence: 99%

Enzymatic hydrolysis of steam-exploded sugarcane bagasse by adding natural Sapindus peel

You¹,

Wang²,

Jiang³

2015

Proceedings of the 5th International Conference on Advanced Design and Manufacturing Engineering

View full text Add to dashboard Cite

Steam explosion pretreatment was a feasible method to treat sugarcane bagasse. The results showed that the hemicelluloses can be effectively removed during the pretreatment and increase the ability of enzymatic hydrolysis. The highest glucose yield was achieved at 74.99%, which was increased by 57.86% with the same enzyme loadings 30 FPU/g-cellulose compared with the untreated sugarcane bagasse. The glucose yield of steam-exploded sugarcane bagasse can be significantly improved from 36.46% to 74.99% with the increment of cellulase. Sapindus peel contained natural surfactant can evidently reduce the surface tension of hydrolysis broth and improve the ability of enzymatic hydrolysis at an economic concentration (1.2 g/L). The highest glucose yield was of 80.99% with cellulase loading of 10 FPU/g-cellulose and addition of 1.2 g/L Sapindus peel, which suggest that 10 FPU/g-cellulose was suitable for lignocellulose saccharification industry.

show abstract

“…Non-ionic surfactants such as Tween 20 (polyethylene glycol sorbitan monolaurate) and Tween 80 (polyethylene glycol sorbitan monooleate) have been reported to prevent the non-productive binding of lignin to cellulases [27]. Various effects have been reported for surfactants including alteration in the structure [28,29], stabilization of enzymes thereby preventing their denaturation [29], positive interaction between substrate and enzymes etc. [27].…”

Section: Introductionmentioning

confidence: 99%