Enzymatic hydrolysis of steam-exploded sugarcane bagasse by adding natural Sapindus peel

Abstract

Steam explosion pretreatment was a feasible method to treat sugarcane bagasse. The results showed that the hemicelluloses can be effectively removed during the pretreatment and increase the ability of enzymatic hydrolysis. The highest glucose yield was achieved at 74.99%, which was increased by 57.86% with the same enzyme loadings 30 FPU/g-cellulose compared with the untreated sugarcane bagasse. The glucose yield of steam-exploded sugarcane bagasse can be significantly improved from 36.46% to 74.99% with the increment of cellulase. Sapindus peel contained natural surfactant can evidently reduce the surface tension of hydrolysis broth and improve the ability of enzymatic hydrolysis at an economic concentration (1.2 g/L). The highest glucose yield was of 80.99% with cellulase loading of 10 FPU/g-cellulose and addition of 1.2 g/L Sapindus peel, which suggest that 10 FPU/g-cellulose was suitable for lignocellulose saccharification industry.