Effect of supra-nutritional selenium-enriched probiotics on hematobiochemical, hormonal, and Doppler hemodynamic changes in male goats

Mandour, Ahmed S.; Samir, Haney; El-Beltagy, Marwa A.; Abdel-Daim, Mohamed M.; Watanabe, Izumi; Ma, Danfu; Matsuura, Katsuhiro; Tanaka, Ryou; Watanabe, Gen

doi:10.1007/s11356-020-08294-2

Cited by 28 publications

(21 citation statements)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the current study, we conducted a dietary Cu restriction protocol to reduce Cu availability by creating a chemical reaction between sulfur and molybdenum in the ruminal uid (Gould andKendall 2011, Scheiber et al 2013). We aimed to highlight, for the rst time, the disruption in Cu-Ch mRNA expression in cardiac tissues in Shiba goats, a native Japanese non-seasonal breeding breed that is considered to be a good model for studying the cardiac blood ow and ruminant animal physiology (Mandour et al 2020a, Samir et al 2020, to nd out whether Cu de ciency is correlated with cardiac measurements and histopathological scoring.…”

Section: Discussionmentioning

confidence: 99%

“…Cardiac tissue samples from each animal were collected, nely chopped, dried overnight at 100 C° in a hot air oven, nely grounded by mortar and pestle, and 100 mg of the mixture was digested with concentrated nitric acid (Suzuki et al 2007). After ltration, the resultant solution was diluted with molecular water and analyzed by ICP-MS using the previously mentioned procedures (Mandour et al 2020a). ICP-MS setting condition was illustrated in the supplementary table (3).…”

Section: Analysis Of Cardiac Tissue Trace Elementsmentioning

confidence: 99%

See 1 more Smart Citation

Expression of cardiac copper chaperone encoding genes and their correlation with cardiac function parameters in goats

et al. 2021

Self Cite

View full text Add to dashboard Cite

The expression of copper chaperone (Cu-Ch) encoding genes enables an in-depth understanding of copper-associated disorders, but no previous studies have been undertaken to highlight Cu-Ch disturbances in heart tissue in ruminants after copper de ciency (CuD). The current study aimed to investigate the Cu-Ch mRNA expression in goat's heart after experimental CuD and highlight their relationship with the cardiac measurements. Eleven male goats were enrolled and divided into the control group (n = 4) and CuD group (treatment, n = 7), which received copper-reducing dietary regimes (molybdenum and sulfur). Heart function was evaluated by electrocardiography and echocardiography, and at the end of the experiment, all animals were sacri ced, and the cardiac tissues were collected for quantitative mRNA expression by real-time PCR. In the treatment group, cardiac measurements revealed increased preload and the existence of cardiac dilatation and signi cant cardiac tissue damage by histopathology. Also, the relative mRNA expression of Cu-Ch encoding genes; ATP7A, CTr1, LOX, COX17, as well as ceruloplasmin (CP), troponin I3 (TNNI3), glutathione peroxidase (GPX1), and matrix metalloprotease inhibitor (MMPI1) genes were signi cantly down-regulated in CuD group. Besides, there was a variable signi cant correlation between investigated genes and cardiac measurements; meanwhile, a signi cant inverse correlation was observed between histopathological score and ATP7B, CTr1, LOX, and COX17. In conclusion, this study revealed that CuD induces cardiac dilatation and alters the mRNA expression of Cu-Ch genes, in addition to TNNI3, GPX1, and MMPI1 that are considered key factors in clinically undetectable CuD-induced cardiac damage in goats which necessitate further studies for feasibility as biomarkers.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Analysis Of Cardiac Tissue Trace Elementsmentioning

confidence: 99%

Expression of cardiac copper chaperone encoding genes and their correlation with cardiac function parameters in goats

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Importantly, few studies are focusing on goat's echocardiography in which female goats, particularly the Saanen breed, are the most studied animal [10,13]. Meanwhile, Shiba goats; a native Japanese non-seasonal breeding animal [39,40] was not investigated, despite being an efficient experimental model for characterization of the blood flow dynamics in ruminants [41][42][43]. To our knowledge, only one reputable study has been conducted on a group of Golden Guernsey and Saanen goats from both sex but the authors did not specify any results for male goats which is inevitable to get accurate echocardiographic reference intervals [21].…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Cardiac Functions Using Full Conventional Echocardiography with Tissue Doppler Imaging before and after Xylazine Sedation in Male Shiba Goats

Mandour

Samir

Yoshida

et al. 2020

Animals

Self Cite

View full text Add to dashboard Cite

The present study aimed to provide a complete conventional echocardiographic protocol in adult male Shiba goats by using two-dimensional, M-mode, Pulsed Wave Doppler, and tissue Doppler imaging (TDI) echocardiography, and to study concomitantly xylazine-induced alteration of cardiac functions in a highly sensitive species. For this purpose, 12 male Shiba goats were included and complete conventional echocardiography from the standard right and left parasternal views was carried to report the echocardiographic data in male Shiba goats, and also before and after xylazine (Pre-Xyl and Post-Xyl) administration (0.05 mg/IM/kg). Results revealed that the full echocardiographic protocol was feasible in all goats through different cardiac windows and good Doppler alignment was achieved with non-significant variability for assessment of the left ventricular dimensions, trans-pulmonary, trans-aortic, and trans-mitral blood flow. The TDI, which was not reported previously in goats, was successfully assessed from the standard left apical view and showed distinct systolic and diastolic patterns. Xylazine administration was found to significantly reduce heart rate, fractional shortening, and cardiac output as well as the Doppler hemodynamic parameters of the pulmonary artery, aortic and mitral inflows (p < 0.05). For TDI, the Post-Xyl group revealed a significant decrease in the myocardial velocities of the septal and lateral wall of the left ventricle. The present study provides, for the first time, complete data of conventional echocardiography in male goats using the full protocol, which is routinely used in pet’s practice. Further, we illustrate in-depth the adverse effect of short-term sedative, xylazine, as used under field conditions and emphasize a simultaneous reduction in both systolic and diastolic cardiac function in goats based on full echocardiography assessment of the heart.

show abstract

“…[ 21 ] Indeed, selenium nanoparticles reportedly improved immune response and stress toughness in fish [ 22 ] as well as augmented blood perfusion, reproductive hormones, and hematobiochemical parameters in goats. [ 23 ]…”

Section: Introductionmentioning

confidence: 99%

Abatement of the dysfunctional hypothalamic–pituitary–gonadal axis due to ciprofloxacin administration by selenium in male rats

Adedara

Awogbindin

Mohammed

et al. 2021

J Biochem & Molecular Tox

View full text Add to dashboard Cite

The present study examined the influence of selenium on ciprofloxacin‐mediated reproductive dysfunction in rats. The research design consisted of five groups of eight animals each. The rats were administered 135 mg/kg body weight of ciprofloxacin per se or simultaneously with selenium at 0.25 and 0.5 mg/kg for 15 uninterrupted days. Antioxidant and inflammatory indices were assayed using the testes, epididymis, and hypothalamus of the animals after sacrifice. Results revealed that ciprofloxacin treatment per se interfered with the reproductive axis as demonstrated by diminished serum hormonal levels, sperm quality, and enzymatic indices of testicular function, which were, however, abrogated following selenium co‐treatment. Besides this, administration of selenium attenuated the depletion of glutathione level, inhibition of catalase, superoxide dismutase, glutathione‐S‐transferase and glutathione peroxidase activities with a concomitant reduction in reactive oxygen and nitrogen species, and lipid peroxidation in ciprofloxacin‐treated in rats. Selenium treatment also mitigated ciprofloxacin‐mediated elevation in nitric oxide level and of myeloperoxidase activity as well as histological lesions in the animals. Overall, selenium attenuated impairment in the male reproductive axis due to ciprofloxacin treatment through abatement of inflammation and oxidative stress in rats.

show abstract

Effect of supra-nutritional selenium-enriched probiotics on hematobiochemical, hormonal, and Doppler hemodynamic changes in male goats

Cited by 28 publications

References 78 publications

Expression of cardiac copper chaperone encoding genes and their correlation with cardiac function parameters in goats

Expression of cardiac copper chaperone encoding genes and their correlation with cardiac function parameters in goats

Assessment of the Cardiac Functions Using Full Conventional Echocardiography with Tissue Doppler Imaging before and after Xylazine Sedation in Male Shiba Goats

Abatement of the dysfunctional hypothalamic–pituitary–gonadal axis due to ciprofloxacin administration by selenium in male rats

Contact Info

Product

Resources

About