Effect of stress-proteins on survival of bone marrow mesenchymal stem cells after intramyocardial transplantation against the background of postinfarction heart remodeling

Abstract

We studied the presence of colony-forming cells in cell culture from rat heart 40 days after experimental myocardial infraction. The mean cellularity in this pathology was 12+/-8 cell/cm2, which is 20-fold lower than in intact myocardium. Transplantation of mesenchymal stem cells into the remodeling myocardium restored the pool of colony-forming cells. This effect depended on the state of transplanted cells. After transplantation of mesenchymal stem cells with low content of stress proteins, 6+/-2 colonies were detected, while after transplantation of cells with high content of hsp70 and hsp60 stress proteins (modified mesenchymal stem cells) 18+/-5 colonies were found, the mean cellularity of the corresponding cultured being 946+/-267 and 1926+/-123 cell/cm2. The positive effect of modified mesenchymal stem cells was observed on days 4 and 7 after transplantation. We conclude that postinfarction remodeling mobilized the total pool of regional stem cells; mesenchymal stem cells with high content of hsp70 and hsp60 demonstrated highest survival rate after intramyocardial transplantation.

Cardiosphere-Derived Cells from Pediatric End-Stage Heart Failure Patients Have Enhanced Functional Activity Due to the Heat Shock Response Regulating the Secretome

“…Since the HSR is elevated in ESHF myocardium and stimulates cytokine secretion in other stem cells , we next determined whether the HSR may play a role on the functional activity of the CDCs by initially establishing their expression pattern in the ESHF and CHD myocardium. Expression of HSF‐1, the HSF master transcriptional activator, was upregulated in ESHF myocardium when compared with CHD myocardium, which also correlated with the activation of the downstream genes HSP60 and HSP70 (HSF‐1: ESHF, n = 4, 0.62 ± 0.1 vs. CHD, n = 5, 0.33 ± 0.064, p = .031; HSP60: ESHF, n = 4, 32.96 ± 3.99 vs. CHD, n = 5, 8.441 ± 2.46, p = .0159; HSP70: ESHF, n = 4, 0.94 ± 0.11 vs. CHD, n = 5, 0.32 ± 0.04, p = .0159, Fig.…”

Application and Progress of Combined Mesenchymal Stem Cell Transplantation in the Treatment of Ischemic Cardiomyopathy

“…Therefore, the higher the number of transplanted cells, the more difficult for cells in the middle of the cell mass to obtain enough oxygen and nutrients and the higher the rate of cell death [ 19 ]. Additionally, a higher number of transplanted cells might stimulate the cellular apoptosis process, which would fail to increase the corresponding cellular survival rate in the infarction region [ 20 ]. Because the relationship between cell dose and outcome of the MSCTT is still controversial, research is focused on improving the survival and treatment outcomes of MSCTT through multiple methods.…”

Nonpharmacological Correction of Hypersympatheticotonia in Patients with Chronic Coronary Insufficiency and Severe Left Ventricular Dysfunction

“…The presence of hsp60 and hsp70 proteins was evaluated by immunoenzymatic method 25 with ELISA Kits (USA) according to manufacturer's protocol. Hsp60 and hsp70 contents were tested in the lysate of lymphocytes isolated from peripheral blood of patients.…”