2018
DOI: 10.1590/1807-2577.08317
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Abstract: Introduction Proper storage conditions and maintenance of viable biological material plays an important role in microbiological research, allowing for the opportunity to conduct future studies. Objective To evaluate the viability of Streptococcus mutans strains that were previously grown and stored under different temperatures for approximately eight years. Material and method In this study, we evaluated 393 bacterial isolates that were stored in a freezer at -80°C (G1) and 200 isolates stored in a freeze… Show more

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Cited by 7 publications
(16 citation statements)
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References 14 publications
(16 reference statements)
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“…Nasal discharges from food handlers may contaminate surrounding environments and surfaces. Decreased contamination in beef burger and beef kofta may be attributed to the effects of freezing, because keeping products at −20°C kills microorganisms [29].…”
Section: Discussionmentioning
confidence: 99%
“…Nasal discharges from food handlers may contaminate surrounding environments and surfaces. Decreased contamination in beef burger and beef kofta may be attributed to the effects of freezing, because keeping products at −20°C kills microorganisms [29].…”
Section: Discussionmentioning
confidence: 99%
“…It has been demonstrated that the trehalose would defend cell proteins against denaturation (Guowei et al 2019). More important, trehalose penetrates the cells and reduces the damaging effects of osmosis during water loss and prevents the development of ice crystals and resultant breakdown of plasma membrane (Cota and Alvim 2018). Concentration of the stabilizer considerably affects the viability of microorganisms during lyophilization.…”
Section: Discussionmentioning
confidence: 99%
“…Zeng et al (2009) has also suggested that loss of viability is consequence of temperature induced plasma membrane damage. As a result, the irreversible damage to bacterial cell membrane leads to unviable cells (Cota and Alvim 2018).…”
Section: Discussionmentioning
confidence: 99%
“…O ácido lático, produzido pelas bactérias, não é diluído ou neutralizado, o que resulta na desmineralização gradual do esmalte externo dos dentes e seu posterior rompimento, permitindo que bactérias penetrem em seu interior. 3,4 A escovação e uso de fio dental, quando utilizados regularmente, são meios eficazes e seguros para remoção mecânica do biofilme. 5 No entanto, esses métodos não são tão eficazes para diminuição de S. mutans de fissuras e superfícies proximais dos dentes, sendo assim, necessário o uso de agentes químicos como coadjuvantes da higienização bucal.…”
Section: Introductionunclassified