Effect of Various Stabilizers on Viability of Lyophilized Pasteurella multocida B:3,4 for Use as Hemorrhagic Septicemia Vaccine

Abstract

Hemorrhagic septicemia (HS) is a devastating disease of cattle and buffaloes. The live aerosol vaccine is the best option to control HS. However, stability and viability of live vaccine is an issue. The present study was conducted to investigate the effect of three extraneous stabilizers trehalose, skimmed milk and lactalbumin on the viability of the live vaccine strain Pasteurella multocida B:3,4. The viability of the strain was evaluated using various concentrations (5, 10, 15 and 20%) of these three stabilizers. Moreover, viability of P. multocida B:3,4 was also determined at four different storage temperatures (-20, 4, 25 and 37°C). The duration of lyophilization cycle was also standardized for highest survival of cells. The data showed that trehalose and lactalbumin ensued percentage of viability as 91.89±0.08 and 80.38±2.57 respectively. Skimmed milk as stabilizer did not prove to defend cells during lyophiliztion and subsequent storage and exhibited cell viability approximately 0.47±0.009%. The study indicated that most effective stabilizer for lyophiliztion of P. multocida B:3,4 was trehalose at 15% concentration and was most suitable temperature for storage of lyophilized P. multocida B:3,4. © 2021 Friends Science Publishers