Effect of steroid hormones on the metabolism of human spermatozoa

Trifunac, Natalie P.; Bernstein, Gail A.

doi:10.1016/0010-7824(81)90080-9

Cited by 6 publications

(1 citation statement)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since there is no significant change in creatine kinase activity after tamoxifen treatment, we speculate that ATP is not only being synthesized, but transported normally in tamoxifen treated rat sperm. High testosterone levels within the caput epididymis appear to play an inhibitory role on mitochondrial functions (Trifunac and Bernstein, 1981). As testosterone levels fall in subsequent sections of epididymis, the inhibitory effect of testosterone is replaced by a conducive estrogenic milieu (Idaomar et al, 1989;Orgebin-Crist et al, 1975).…”

Section: Discussionmentioning

confidence: 99%

Tamoxifen, protein kinase C and rat sperm mitochondria

Saberwal

2003

Cell Biology International

View full text Add to dashboard Cite

Tamoxifen at a dose of 400 microg/kg/day has been reported to reduce the fertility of adult male rats and alter the pattern of cauda sperm motility from forward progressive to circular yawing type. Since sperm motility is powered by mitochondria, the effect of tamoxifen on mitochondrial function was studied. Tamoxifen treatment significantly increased rhodamine 123 fluorescent dye uptake by sperm mitochondria, reflecting an altered mitochondrial membrane potential. ATP and DAG levels, activities of glycolytic enzymes, creatine kinase and PKC all remained unaffected by tamoxifen. This is also the first report describing the presence of PKC alpha and beta in rat sperm. Morphological and biochemical integrity of sperm membranes was determined by electron microscopy and malondialdehyde levels, which were unaltered after tamoxifen treatment. This study indicates that the altered sperm motility induced by tamoxifen is accompanied by changes in mitochondrial membrane potential, but in the absence of any detectable change in membrane integrity, lipid peroxidation, ATP levels and activities of glycolytic enzymes, creatine kinase and PKC.

show abstract

Section: Discussionmentioning

confidence: 99%