Effect of Standardized Boesenbergia pandurata Extract and Its Active Compound Panduratin A on Skin Hydration and Barrier Function in Human Epidermal Keratinocytes

Abstract

The skin plays a key role in protecting the body from the environment and from water loss. Cornified envelope (CE) and natural moisturizing factor (NMF) are considered as the primary regulators of skin hydration and barrier function. The CE prevents loss of water from the body and is formed by cross-linking of several proteins. Among these proteins, filaggrin is an important protein because NMF is produced by the degradation of filaggrin. Proteases, including matriptase and prostasin, stimulate the generation of filaggrin from profilaggrin and caspase-14 plays a role in the degradation of filaggrin. This study elucidated the effects of an ethanol extract of Boesenbergia pandurata (Roxb.) Schltr., known as fingerroot, and its active compound panduratin A on CE formation and filaggrin processing in HaCaT, human epidermal keratinocytes. B. pandurata extract (BPE) and panduratin A significantly stimulated not only CE formation but also the expression of CE proteins, such as loricrin, involucrin, and transglutaminase, which were associated with PPARα expression. The mRNA and protein levels of filaggrin and filaggrin-related enzymes, such as matriptase, prostasin, and caspase-14 were also up-regulated by BPE and panduratin A treatment. These results suggest that BPE and panduratin A are potential nutraceuticals which can enhance skin hydration and barrier function based on their CE formation and filaggrin processing.

Hepatoprotectivity of Panduratin A against liver damage: In vivo demonstration with a rat model of cirrhosis induced by thioacetamide

“…Panduratin A is one of the chalcones that was isolated from Boesenbergia rotunda rhizomes. This compound has been reported to serve as enhancement of skin hydration , antidengue , antibacterial , anticancer , and anti‐inflammatory functions.…”

Fermented black rice and blueberry with Lactobacillus plantarum MG4221 improve UVB-induced skin injury

“…The skin barrier is the outermost layer of the epidermis, protecting skin from harmful external substances and preventing moisture evaporation (Sandilands et al, 2009). In SC, the cornified envelope (CE) is an insoluble protein structure that plays a pivotal role in skin hydration and skin barrier function (Woo et al, 2015). Transglutaminase (TGM) catalyses CE formation to prevent water loss, with its main substrates being CE proteins such as filaggrin (FLG), involucrin (INV), and loricrin (LOR) (Lorz et al, 2019;Woo et al, 2015).…”

“…In SC, the cornified envelope (CE) is an insoluble protein structure that plays a pivotal role in skin hydration and skin barrier function (Woo et al, 2015). Transglutaminase (TGM) catalyses CE formation to prevent water loss, with its main substrates being CE proteins such as filaggrin (FLG), involucrin (INV), and loricrin (LOR) (Lorz et al, 2019;Woo et al, 2015). In particular, FLG is a key marker of skin hydration because of its involvement in the synthesis of natural moisturising factors (Woo et al, 2015).…”

Stimulatory Effects ofPaederia foetidaFlower Absolute on the Skin Wound and Barrier Repair Activities of Keratinocytes

“…[19 -23] Several recent studies have demonstrated that plants and their extracts can improve skin wound and barrier repair functions. [24][25][26][27][28] However, PF flower absolute has not been previously investigated in this context.…”

“…PF and its extracts have been shown to have various bioactivities, which include antidiabetic, anti‐inflammatory, antimicrobial, anticancer, antitussive, and antioxidant activities [19–23] . Several recent studies have demonstrated that plants and their extracts can improve skin wound and barrier repair functions [24–28] . However, PF flower absolute has not been previously investigated in this context.…”