Effect of soybean oil and soybean lecithin on intestinal lipid composition and lipid droplet accumulation of rainbow trout, Oncorhynchus mykiss Walbaum

Criteria for Safe Use of Plant Ingredients in Diets for Aquacultured Fish

“…Using rainbow trout, Rinchard et al (2007) showed that both growth and survival of rainbow trout with initial weight 182g was superior when soy lecithin (14%) was used as lipid source compared to soybean oil, linseed oil and fish oil. Likewise, large amounts of lipid droplets have been reported to accumulate in livers of European sea bass (Mourente et al 2005b) and gilthead sea bream (Wassef et al 2007), in intestinal enterocytes of gilthead sea bream , Arctic charr (Olsen et al 1999b;, rainbow trout (Olsen et al 2003) and Atlantic salmon (Olsen and Ringø unpublished data). Many of these experiments do seem to have at least two factors in common.…”

“…Firstly, the protein sources are either highly purified containing very little or no PL, or they use small amounts of fishmeal that is diluted with gluten and wheat to lower the contribution of PL from fishmeal. As for marine larvae, lipid droplets, at least in enterocytes, are removed by adding exogenous phospholipids like soybean lecithin (Olsen et al 1999b;Caballero et al 2003;Olsen et al 2003). Secondly, there is often a direct correlation between lipid droplet accumulation and increased content of plant oils such as linseed and soybean in the diets.…”

Biochemical composition of copepods for evaluation of feed quality in production of juvenile marine fish

“…Quantitative analysis of fatty acid methyl esters were carried out 294 by gas liquid chromatography using a HP 5890 gas chromatograph (Hewlett Packard 295 Labs Inc., Palo Alto, CA, USA) equipped with a J&N Scientific Inc DB-23 fused silica 296 column (30 m x 0.25 mm i.d.) as described by Olsen et al (2004). Abbreviations for 297 lipid classes and fatty acids used in the text are given in Table 2.…”

Dietary Effect of Soybean (Glycine max) Products on Gut Histology and Microbiota of Fish

“…With the inclusion of plant oils into aquafeeds, fish are faced with several challenges some of which will affect intestinal health and function. One of the most striking effects is the massive accumulation of lipid droplets in enterocytes of fish species such as Arctic charr , gilthead seabream (Caballero et al, 2003), rainbow trout (Caballero et al, 2002;Olsen et al, 2003) and Atlantic salmon (Olsen et al, unpublished data) fed high levels of plant oils. The accumulations may amount to more than 60% of cellular volume which hampers gut functions ( Figure 5).…”