Effect of some phytohormones on growth characteristics of Chlorella sorokiniana IAM-C212 under photoautotrophic conditions

Abstract

The effects of some phytohormones [indole acetic acid (IAA), indole butyric acid (IBA), gibelleric acid (GA 3), and kinetin] on cell dry weight, cell number, cell size, protein and chlorophyll contents of Chlorella sorokiniana IAM-C212 were investigated under photoautotrophic conditions. Treatment with IAA (15 mg/l) and IBA (15 mg/l) resulted in significantly higher dry cell weight and cell number than the control (P < 0.05) but there were no significant effects of GA 3 and kinetin on cell growth as expressed by dry cell concentration (g/l) and cell population (cells/ml). Treatment with IAA at a concentration of 10 or 15 mg/l gave the highest cell dry concentration of 4.68 g/l after eight days of cultivation, which is more than 9 times higher than the value obtained in the control culture (without phytohormone). The optimum concentration of each of the phytohormones for C. sorokiniana cell enlargement was 20 mg/l. At this concentration, the average cell sizes were 81.07, 78.67, 78.07, 66.90 and 68.1 µm for GA 3 , kinetin, IAA, IBA and control, respectively. Addition of 15 mg/l of IAA or GA 3 to the culture resulted in significantly higher extractable chlorophyll contents than the control (P < 0.05) but the effects of IBA and kinetin were not significant (P > 0.05). The protein contents of the cells cultivated with 20 or 10 mg/l of GA 3 , 15 mg/l of kinetin, and 15 mg/l of IBA or IAA were 46.64, 45.83 and 45.81%, respectively. In the control experiment, the protein content was 43.38% after eight days of cultivation, showing that treatment with these phytohormones had no significant effect on the protein contents of the cells (P > 0.05). Combination of IBA and GA 3 exhibited synergistic effect on growth and productivity of C. sorokiniana but there was no synergistic effect when IAA was combined with either GA 3 or kinetin.

Effect of Phytohormones Supplementation under Nitrogen Depletion on Biomass and Lipid Production of Nannochloropsis oceanica for Integrated Application in Nutrition and Biodiesel

“…In the case of hormone supplementation, the present study showed a gradual increase in growth by increasing of IAA up to T5 (which represented 129.0% higher than the control), then showed gradual reduction at higher IAA concentrations. The present results agree with the data obtained by Fabian et al [34] who reported that the treatment of Chlorella sorokiniana with 10 mg L −1 IAA results in the highest dry weight, representing about 9-folds higher than the control. A similar effect was recorded with Parachlorella kessleri, where a positive correlation was recorded with IAA and microalgal growth [35].…”

Enhanced Extracellular Polysaccharide Production and Self-Sustainable Electricity Generation for PAMFCs byScenedesmussp. SB1

“…SB1 (Figure S1). Studies on the influence of IAA on the biomass growth in Chlorella sorokiniana have already been reported by Ozioko et al in 2015, where a 10 mg/L IAA has resulted in a 9.5-fold increase in the cell concentration.…”

Plant growth regulator and soil fertilizer improve production and growing stage of Brachiaria decumbens

“…Corroborating withBryant, Edwards, and Robinson (2016), the use of nitrogen fertilization (50 kg N/ha) combined with sprays of gibberellin increased DM yields. It is worth emphasizing the influence of the hormonal compound used in the formation of structures such as leaf, stem and root, already mentioned in experiment 1(Tables 4 and 5).Chlorophyll content, protein, cell growth and biomass production were more pronounced in plants under the synergistic effect of the addition of auxins and gibberellins, suggesting an increase in the cellular concentrations of these compounds(Ozioko, Chiejina, & Ogbonna, 2015). The increase in the contents of chlorophyll a with the use of growth regulator, combined with the result of mass yield, justifies the efficiency of use of exogenous compounds in the regulation of plant development as they exert functions in the signaling network of photosynthetic processes.…”