Effect of some mobile phase additives on the retention characteristics of different solute types on reversed-phase media. II

McCrossen, Sean; Simpson, C. F.

doi:10.1016/0021-9673(94)00953-7

Cited by 6 publications

(1 citation statement)

References 12 publications

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“…Many ways exist to reduce the number or effect of silanols on the surface and, hence, the tailing of chromatographic peaks. These include end capping or other ways of achieving full coverage of the silica, using mobile-phase additives, − lowering the pH of the mobile phase, , and removing metal impurities from the silica. , While useful for reducing tailing, these techniques do not entirely eliminate tailing, and its mechanism is not fully understood.…”

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Single-Molecule Resolution and Fluorescence Imaging of Mixed-Mode Sorption of a Dye at the Interface of C₁₈ and Acetonitrile/Water

Ludes

Wirth

2001

Anal. Chem.

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Single-molecule imaging is used for the first time to study the cationic dye, 1,1'-dioctadecyl-3,3,3'3'-tetramethylindocarbocyanine perchlorate (DiI), at the chromatographic interface consisting of acetonitrile/water and a hydrocarbon monolayer (C18) covalently bound to silica. Autocorrelations of burst data agree with our previous single-molecule counting results, showing that most dye molecules are diffusing and that there is a rare specific adsorption site associated with a 0.07-s desorption time. These autocorrelations go further in detecting an even rarer specific adsorption event associated with a 2.6-s desorption time. The latter desorption time would contribute much more significantly to peak tailing in chromatography. In water, the populations of DiI at these two specific adsorption sites are shown to be 11% and 4%, respectively, for the weaker and stronger sites, relative to the diffusing population of DiI. In 60% acetonitrile/water, the relative populations of the specific adsorption sites are 11% and 17%, showing that acetonitrile enhances the population of the stronger specific adsorption site. Fluorescence movies of single and multiple molecules link the stronger specific adsorption sites to specific locations on the surface. The imaging makes rare observations frequent by pinpointing where the events occur spatially. This ability to observe rare events by imaging reveals the presence of a third type of specific adsorption site, for which DiI has a desorption time in excess of 20 s.

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Single-Molecule Resolution and Fluorescence Imaging of Mixed-Mode Sorption of a Dye at the Interface of C₁₈ and Acetonitrile/Water

Ludes

Wirth

2001

Anal. Chem.

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Ionic Samples: Reversed‐Phase, Ion‐Pair, and Ion‐Exchange HPLC

Snyder

Kirkland

Glajch

1997

Practical HPLC Method Development

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Liquid chromatographic separation of the alkaloids in coptis-evodia herb couple

Chu

Sheu

1996

Journal of Chromatography A

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Effect of some mobile phase additives on the retention characteristics of different solute types on reversed-phase media. II

Cited by 6 publications

References 12 publications

Single-Molecule Resolution and Fluorescence Imaging of Mixed-Mode Sorption of a Dye at the Interface of C₁₈ and Acetonitrile/Water

Single-Molecule Resolution and Fluorescence Imaging of Mixed-Mode Sorption of a Dye at the Interface of C₁₈ and Acetonitrile/Water

Ionic Samples: Reversed‐Phase, Ion‐Pair, and Ion‐Exchange HPLC

Liquid chromatographic separation of the alkaloids in coptis-evodia herb couple

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Effect of some mobile phase additives on the retention characteristics of different solute types on reversed-phase media. II

Cited by 6 publications

References 12 publications

Single-Molecule Resolution and Fluorescence Imaging of Mixed-Mode Sorption of a Dye at the Interface of C18 and Acetonitrile/Water

Single-Molecule Resolution and Fluorescence Imaging of Mixed-Mode Sorption of a Dye at the Interface of C18 and Acetonitrile/Water

Ionic Samples: Reversed‐Phase, Ion‐Pair, and Ion‐Exchange HPLC

Liquid chromatographic separation of the alkaloids in coptis-evodia herb couple

Contact Info

Product

Resources

About

Single-Molecule Resolution and Fluorescence Imaging of Mixed-Mode Sorption of a Dye at the Interface of C₁₈ and Acetonitrile/Water

Single-Molecule Resolution and Fluorescence Imaging of Mixed-Mode Sorption of a Dye at the Interface of C₁₈ and Acetonitrile/Water