Liquid chromatographic separation of the alkaloids in coptis-evodia herb couple

Chu, Chan-Yeo; Sheu, Shuenn‐Jyi

doi:10.1016/s0021-9673(96)00621-8

Cited by 20 publications

(19 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This method has several advantages over the other analytical procedures, such as high-performance liquid chromatography (HPLC) [11,12] and spectrophotometry [13,14] , such as simple pretreatment of samples, low cost, and a large number of samples that can be screened in parallel. However, some disadvantages were found such as the lower precision and sensitivity of the method compared to the HPLC.…”

Section: Determination Of Berberine Content In the Stem Extractsmentioning

confidence: 99%

Determination of Berberine Content in the Stem Extracts of <i>Coscinium fenestratum</i> by TLC Densitometry

2006

View full text Add to dashboard Cite

Objective: To develop the optimal extraction procedure (i.e. maceration, percolation or Soxhlet extraction) and thin- layer chromatographic (TLC)-densitometric method for the determination of berberine content of Coscinium fenestratum. Materials and Methods: Maceration, percolation and Soxhlet extraction techniques were used to extract alkaloids from dried stems of C. fenestratum. The solvents used were 50 and 80% ethanol. Crude extracts and berberine content recovered from the TLC fingerprint were evaluated for chemical components of each extraction method. Precoated silica gel GF₂₅₄ plates were used as stationary phase while butanol:glacial acetic acid:water (14:3:4) was used as a mobile phase. Detection and quantitation of berberine were performed by densitometry at the wavelength of 415 nm over the linearity range of 240–840 ng (r² = 0.9982). The relative standard deviations from intraday and interday precisions were less than 4.13%. Results: The recovery of standard berberine was 97.58–98.71% (%RSD = 3.85), and the limit of detection and quantitation were 25 and 50 ng/spot, respectively. Eighty percent ethanol gave a higher content of berberine than 50% ethanol. Berberine contents from maceration, percolation and Soxhlet extraction with 80% ethanol were 3.37± 0.30, 3.08± 0.38 and 2.67± 0.27% w/w, respectively. Conclusion: The TLC-densitometric method was simple, accurate and precise for quantitating berberine in the stem extract of C. fenestratum. Maceration with 80% ethanol gave the highest content of berberine in the extract. TLC of the extracts from different methods showed a similar pattern.

show abstract

Section: Determination Of Berberine Content In the Stem Extractsmentioning

confidence: 99%

Determination of Berberine Content in the Stem Extracts of <i>Coscinium fenestratum</i> by TLC Densitometry

2006

View full text Add to dashboard Cite

show abstract

“…However, in the Pharmacopoeia of the People's Republic of China (Chinese Pharmacopoeia Commission, 2010), berberine is monitored as the signal marker for quality control of Zuojin-related products. Thinlayer chromatography (Shen et al, 1993), capillary electrophoresis (Lee et al, 1997;Wang et al, 2006), HPLC DAD (Chu et al, 1996), LC-MS (Luo et al, 2005), and UPLC-TUV (Gao et al, 2010) were established for analysis of isoquinoline and indolequinazoline alkaloids in this formula for quality control. To date, although, there are a few studies on the determination of alkaloids of the herb couple by HPLC-UV (Chu et al, 1996;Luo et al, 2005), no general fingerprint information is available.…”

Section: Introductionmentioning

confidence: 99%

“…Thinlayer chromatography (Shen et al, 1993), capillary electrophoresis (Lee et al, 1997;Wang et al, 2006), HPLC DAD (Chu et al, 1996), LC-MS (Luo et al, 2005), and UPLC-TUV (Gao et al, 2010) were established for analysis of isoquinoline and indolequinazoline alkaloids in this formula for quality control. To date, although, there are a few studies on the determination of alkaloids of the herb couple by HPLC-UV (Chu et al, 1996;Luo et al, 2005), no general fingerprint information is available. Therefore, the objective of this research is to establish a HPLC DAD method both for fingerprinting analysis to verify the quality consistency of CEC and its related TCMPs, and for simultaneous determination of seven major bioactive compounds for providing comprehensive analysis information.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Coptidis Rhizoma–Euodiae Fructus couple and Zuojin products based on HPLC fingerprint chromatogram and simultaneous determination of main bioactive constituents

Gào

Yang

Marriott

2013

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Coptidis Rhizoma-Euodiae Fructus couple (CEC) is a classic traditional Chinese medicine preparation consisting of Coptidis Rhizoma and Euodiae Fructus at the ratio of 6:1, and used to treat gastro-intestinal disorders. Alkaloids are the main bioactive component. This research provides comprehensive analysis information for the quality control of CEC. Objective: To develop a high-performance liquid chromatography-diode array detection fingerprint for chemical composition characteristics of CEC and its products. Materials and methods: The samples were separated with a Gemini C 18 column by using gradient elution with water-formic acid (100:0.03) and acetonitrile as mobile phase. Flow rate was 1.0 mL/ min and detection wavelength was 250 nm. Similarity analysis and principal component analysis (PCA) were employed to evaluate quality consistencies of analytes. Mean chromatograms and correlation coefficients of analytes were calculated by the software ''Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine''. Results: Fingerprint chromatogram comparison determined 20 representative general fingerprint peaks, and the fingerprint chromatogram resemblances are all better than 0.988. Consistent results were obtained to show that CEC and its related samples could be successfully divided into three groups. Contribution plots generated by PCA were performed to interpret differences among the sample groups while peaks which significantly contributed to classification were identified. Seven bioactive constituents in the samples were verified by quantitative analysis. Discussion and conclusion: The chromatographic fingerprint with similarity evaluation and PCA assay combined with quantification of seven compounds could be utilized as a quality control method for the herbal couple.

show abstract

“…The protoberberine alkaloids in C. rhizoma and indolequinoline alkaloids in E. fructus were analyzed by high-performance liquid chromatography (HPLC) separately [14,23,24]. Several methods were reported for the determination of protoberberine and indolequinoline alkaloids in herb couple of C. rhizoma and E. fructus, such as HPLC [25], HPLC-mass spectrometry (MS) [3], and capillary electrophoresis (CE) [26]. However, the complicated solvent systems (a mixture of acetonitrile, methanol, and sodium dodecyl sulfate-NaOAc-HOAc buffer or NaOAc-HOAc buffer) and gradient elution provided inconvenient application.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of protoberberine and indolequinoline alkaloids in Zuojin pill extract by high-performance liquid chromatography

Zhang

Wang

2013

Acta Chromatographica

View full text Add to dashboard Cite

Liquid chromatographic separation of the alkaloids in coptis-evodia herb couple

Cited by 20 publications

References 13 publications

Determination of Berberine Content in the Stem Extracts of <i>Coscinium fenestratum</i> by TLC Densitometry

Determination of Berberine Content in the Stem Extracts of <i>Coscinium fenestratum</i> by TLC Densitometry

Evaluation of Coptidis Rhizoma–Euodiae Fructus couple and Zuojin products based on HPLC fingerprint chromatogram and simultaneous determination of main bioactive constituents

Simultaneous analysis of protoberberine and indolequinoline alkaloids in Zuojin pill extract by high-performance liquid chromatography

Contact Info

Product

Resources

About