Determination of Berberine Content in the Stem Extracts of <i>Coscinium fenestratum</i> by TLC Densitometry

Abstract

Objective: To develop the optimal extraction procedure (i.e. maceration, percolation or Soxhlet extraction) and thin- layer chromatographic (TLC)-densitometric method for the determination of berberine content of Coscinium fenestratum. Materials and Methods: Maceration, percolation and Soxhlet extraction techniques were used to extract alkaloids from dried stems of C. fenestratum. The solvents used were 50 and 80% ethanol. Crude extracts and berberine content recovered from the TLC fingerprint were evaluated for chemical components of each extraction method. Precoated silica gel GF₂₅₄ plates were used as stationary phase while butanol:glacial acetic acid:water (14:3:4) was used as a mobile phase. Detection and quantitation of berberine were performed by densitometry at the wavelength of 415 nm over the linearity range of 240–840 ng (r² = 0.9982). The relative standard deviations from intraday and interday precisions were less than 4.13%. Results: The recovery of standard berberine was 97.58–98.71% (%RSD = 3.85), and the limit of detection and quantitation were 25 and 50 ng/spot, respectively. Eighty percent ethanol gave a higher content of berberine than 50% ethanol. Berberine contents from maceration, percolation and Soxhlet extraction with 80% ethanol were 3.37± 0.30, 3.08± 0.38 and 2.67± 0.27% w/w, respectively. Conclusion: The TLC-densitometric method was simple, accurate and precise for quantitating berberine in the stem extract of C. fenestratum. Maceration with 80% ethanol gave the highest content of berberine in the extract. TLC of the extracts from different methods showed a similar pattern.

Berberine Inhibits Growth and Induces G1 Arrest and Apoptosis in Human Cholangiocarcinoma QBC939 Cells

“…When cells reached 60% -70% confluence, the cells were cultured in serum free DMEM for 24 h in order to reach synchronization, and then the cells were added into the DMEM (10% FCS in media) with different concentrations (10,40, and 80 μM) of berberine for another 24 and 48 h.…”

“…Cultured cells were treated with or without different concentrations (10,40, and 80 μM) of berberine for another 24 and 48 h, and LDH activity was assayed by absorbance change at a wavelength of 440 nm with an LDH assay kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China).…”

“…Berberine is an isoquinoline alkaloid, obtained from a number of important medicinal plant species, such as Berberis aristata, Berberis vulgaris (barberry), Coscinium fenestratum, and Berberis aquifolium (9,10). Berberine exhibits a variety of biochemical and pharmacological activities such as antibacterial (11), antihypertensive (12), anti-inflammatory (13), antidiabetic (14), and anti-hyperlipidemic activities (15).…”

Physico Chemical Characterization and Anti Inflammatory Activity of Stem Extracts of Berberis aristata DC and Cosinium fenestratum Linn in Carrageenan Induced Wistar Rats

“…Ayurvedic Pharmacopeia of India correlates Daruharidra to Berberis aristata DC of family Berberidaceae, a spinous shrub native to mountains of North India and Nepal and commonly known as 'Daruhaldi' [4,5] . In South India, Cosinium fenestratum is traded as substitute for B. aristata in the name of Daruharidra [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] and native to Western Ghats as 'tree turmeric'. Daruharidra is useful in a vast range of disease conditions including inflammations, wounds, ulcers, jaundice, burns, skin diseases, abdominal disorders, diabetes, fever and general debility [2,6,7] .…”

The Enhanced Carbamate Adsorption of Modified Bentonite with Coscinium fenestratum

“…C. fenestratum extract has antifungal, antibacterial, antiyeast, and antiproliferative activities [13][14][15]. Berberine is the main isoquinoline alkaloid that was presented the natural organic monovalent cation in C. fenestratum, and involves the minor alkaloids such palmatine, tetrahydroplamatine, cerbanine and jatrorhizine, etc [16].…”