Effect of soil extracts concentration on specific growth rate and lipid content of chlorella vulgaris in bolds basal medium

Idris, Ani; Atta, Mostafa A.; Bukhari, Ali

doi:10.11113/mjfas.v10n2.259

Cited by 3 publications

(4 citation statements)

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“…The soil has an oligotrophic environment; it contains all the necessary elements for the optimum growth of microalgae when cultivated in an artificial medium [ 59 ]. Soil contains many free enzymes that play a critical role in catalyzing the reactions leading to the decomposition of organic matter that stimulate microalgal growth [ 60 ].…”

Section: Discussionmentioning

confidence: 99%

Kinetics Growth and Recovery of Valuable Nutrients from Selangor Peat Swamp and Pristine Forest Soils Using Different Extraction Methods as Potential Microalgae Growth Enhancers

et al. 2021

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Soil extracts are useful nutrients to enhance the growth of microalgae. Therefore, the present study attempts for the use of virgin soils from Peninsular Malaysia as growth enhancer. Soils collected from Raja Musa Forest Reserve (RMFR) and Ayer Hitam Forest Reserve (AHFR) were treated using different extraction methods. The total dissolved nitrogen (TDN), total dissolved phosphorus (TDP), and dissolved organic carbon (DOC) concentrations in the autoclave methods were relatively higher than natural extraction with up to 132.0 mg N/L, 10.7 mg P/L, and 2629 mg C/L, respectively for RMFR. The results of TDN, TDP, and DOC suggested that the best extraction methods are autoclaved at 121 °C twice with increasing 87%, 84%, and 95%, respectively. Chlorella vulgaris TRG 4C dominated the growth at 121 °C twice extraction method in the RMRF and AHRF samples, with increasing 54.3% and 14%, respectively. The specific growth rate (µ) of both microalgae were relatively higher, 0.23 d−1 in the Ayer Hitam Soil. This extract served well as a microalgal growth promoter, reducing the cost and the needs for synthetic medium. Mass production of microalgae as aquatic feed will be attempted eventually. The high recovery rate of nutrients has a huge potential to serve as a growth promoter for microalgae.

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Section: Discussionmentioning

confidence: 99%

Kinetics Growth and Recovery of Valuable Nutrients from Selangor Peat Swamp and Pristine Forest Soils Using Different Extraction Methods as Potential Microalgae Growth Enhancers

et al. 2021

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“…Modified media, which contain SEs, may show outstanding results compared to artificial ones. SEs are thought to have a significant impact in the augmentation of microalgae growth due to their composition [2].…”

Section: The Effects Of Modified Ses and Incubation Temperatures On T...mentioning

confidence: 99%

“…Soils are dynamic and diverse natural ecosystems composed of rich organic matter derived from water, minerals, and living organism residues undergoing decomposition processes [1]. Some soils provide an eutrophic environment containing all the fundamental nutrients ideal for microalgae growth relative to artificial media [2]. Artificial seawater media for microalgae growth were developed by eliminating soil extracts (SEs) and substituting them with vitamins, trace metals, chelators, and other organic additives, as initially revealed by [3].…”

Section: Introductionmentioning

confidence: 99%

Growth-promoting effects of marine microalgae species using tropical forest soil extracts

Arumugam¹,

Suhaila²,

Abdullah³

et al. 2022

J Appl Biol Biotech

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Microalgae are a source of high-value substances beneficial for industrial applications. To augment the mass production of microalgae in an artificial culture medium, growth-promoting nutrients are essential. The effect of soil extracts (SEs) on four microalgae species was investigated in this study. Artificial Conway media were supplemented with SE collected from the Ayer Hitam Forest Reserve and Raja Musa Forest Reserve (RM), tested using a microplate-incubation technique. The samples were cultured for nine days at 25°C and 30°C, with a light intensity of 33.75 mol photons m −2 seconds −1 and a 12-hour light/dark cycle. Incubation at 25°C showed a higher specific growth rate (SGR) for Nannochloropsis oculata (0.32-0.39 d −1 ), while, at 30°C, a higher SGR was observed for Dunaliella tertiolecta in all soil treatments (0.31-0.34 d −1 ). In terms of SEs, media + RM SE showed a higher SGR from 0.25 to 0.39 d −1 for four microalgae. Chlorella sorokiniana and N. oculata grew well at 25°C in all soil treatments, while D. tertiolecta and Dunaliella primolecta grew well at 30°C. This study shows that the RM medium was the optimum soil treatment for all microalgae tested due to the higher concentration of organic matter.

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“…The use of soil extracts can be sustainable and cheaper support for mass cultivation of microalgae in addition to commercial medium [31]. Since the soil extracts contain the presence of important nutrients for microalgae growth such as carbon, nitrogen, phosphorus, and trace elements due to the oligotrophic nature [32], they assist the formation of protein which is vital for microalgae cultivation. The occurrence of trace elements such as phosphorus and calcium on the other hand stimulates an accelerated rate of cell division and specific growth rate of microalgae resulting in increased cell density.…”

Section: Effects Of Soil Extracts On the Growth Of Microalgaementioning

confidence: 99%

Malaysian Virgin Soil Extracts as Natural Growth Enhancer for Targeted Green Microalgae Species

et al. 2022

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The microalgae-based industries are trending upwards, particularly as the feed ingredient for aquaculture. Therefore, a sustainable and reasonably priced source of nutrients to support the mass cultivation of microalgae is in great demand. The present study explored the feasibility of using extracts from virgin soil as natural growth-promoting nutrients for the cultivation of Nannochloropsis oculata, Nannochloropsis oceanica, and Chlorella sorokiniana. The extracts were obtained from Bera Lake Forest using five different treatment methods. The greatest retrieval of dissolved organic carbon, total dissolved nitrogen, and total dissolved phosphorus were observed with the autoclave treatment method at 121 °C twice, yielding a respective concentration of 336.56 mg/L, 13.40 mg/L, and 0.14 mg/L, respectively. The highest growth was recorded with Nannochloropsis oculata resulting in an optical density of 0.488 ± 0.009 (×103 cell mL−1), exhibiting 43% and 44% enhanced growth in comparison to Nannochloropsis oceanica and Chlorella sorokiniana, respectively. The specific growth rate (0.114 a ± 0.007 d−1) was the highest for Nannochloropsis oculata when the 24 h-extraction method was used, whereas the utilization of the autoclave 121 °C twice treatment method contributed to the highest specific growth of Nannochloropsis ocenica (0.069 a ± 0.003 d−1) and Chlorella sorokiniana (0.080 a ± 0.001 d−1). Collectively, these findings suggested that the addition of soil extracts which is sustainable and inexpensive promoted the growth of microalgae compared to the control system. A further study investigating the optimum culture conditions for enhanced microalgae growth will be carried out for the mass production of microalgae biomass.

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Effect of soil extracts concentration on specific growth rate and lipid content of chlorella vulgaris in bolds basal medium

Cited by 3 publications

References 0 publications

Kinetics Growth and Recovery of Valuable Nutrients from Selangor Peat Swamp and Pristine Forest Soils Using Different Extraction Methods as Potential Microalgae Growth Enhancers

Kinetics Growth and Recovery of Valuable Nutrients from Selangor Peat Swamp and Pristine Forest Soils Using Different Extraction Methods as Potential Microalgae Growth Enhancers

Growth-promoting effects of marine microalgae species using tropical forest soil extracts

Malaysian Virgin Soil Extracts as Natural Growth Enhancer for Targeted Green Microalgae Species

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