Effect of Sodium Fluoride on Growth of Human Diploid Cells in Culture

Oguro, Akira; Cervenka, J; Horii, Kin-ichi

doi:10.1111/j.1600-0773.1990.tb00854.x

Cited by 17 publications

(8 citation statements)

References 9 publications

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“…[8] It is now an established fact that fluoride ingestion over a period of time can affect the structure and function of cells, tissues, organs, and systems resulting in a variety of clinical manifestations. The various hard tissue effects of fluorosis are hypomineralization of enamel, dentin,[910] hypercementosis,[11] recession of alveolar crest,[11] root resorption,[12] and hypermineralization,[13] while the soft tissue changes include inhibition of Type I collagen synthesis,[14] degree of cross linking,[14] fibroblast growth inhibition,[15] lethal effects on fibroblasts,[161718] and morphologic changes. [15]…”

Section: Introductionmentioning

confidence: 99%

Fluorosed fibroblast attachment on fluorosed and nonfluorosed teeth after SRP and EDTA root biomodification

Girotra

Vandana

2014

J Indian Soc Periodontol

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Background:Fluorosis causes mineralization changes in the tooth and can lead to morphologic alterations of fibroblasts. To understand the effect of fluorosis on periodontal healing, the initial step during healing, such as fibroblast attachment to the root surface, needs to be evaluated. Hence, the objective of the present study was to study the attachment of fluorosed fibroblasts on the fluorosed and nonfluorosed root fragments.Materials and Methods:A total of 56 fluorosed and nonfluorosed, periodontally healthy and diseased tooth roots were obtained and allotted to eight groups: Fluorosed and nonfluorosed healthy controls (FH and NFH, respectively), fluorosed and nonfluorosed diseased controls (FD and NFD, respectively), fluorosed and nonfluorosed roots treated with scaling and root planing (FD + S and NFD + S, respectively), and similar groups treated with scaling and root planing and 24% ethylenediaminetetraacetic acid (EDTA) gel application for 2 min (FD + SE and NFD + SE, respectively). After the respective treatment, the root fragments were incubated in the human periodontal ligament fibroblast cells obtained and cultured from freshly extracted healthy human fluorosed premolar tooth root.Results:In the nonfluorosed roots category, greater attachment was found in the untreated nonfluorosed diseased (P = 0.036) and SRP-treated nonfluorosed diseased groups (P = 0.008) as compared to the nonfluorosed healthy group. While in the fluorosed roots category, no significant difference was observed in FL-FA (P > 0.05) within the group. However, no attachment was observed in EDTA-treated fluorosed root fragments. When fluorosed groups were compared to nonfluorosed groups, no significant changes were noted between the groups.Conclusion:SRP proves to be a standard requirement for fibroblast attachment to occur both in fluorosed and nonfluorosed roots. Although there was no significant difference in attachment between SRP and SRP + EDTA among fluorosed roots, EDTA does not seem to be a promising agent for root biomodification in fluorosed roots in a given concentration and time of treatment.

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Section: Introductionmentioning

confidence: 99%

Fluorosed fibroblast attachment on fluorosed and nonfluorosed teeth after SRP and EDTA root biomodification

Girotra

Vandana

2014

J Indian Soc Periodontol

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“…The two reasons that lead to this study are, the in vitro toxic effects of fluoride on morphology and functions of fibroblasts [8][9][10][11] and difference in therapeutic response of fluorosed and nonfluorosed roots to nonsurgical periodontal treatment. [22,23] Following periodontal therapy, the initial step in periodontal wound healing such as fibroblast attachment between fluorosed and nonfluorosed roots requires to be evaluated.…”

Section: Discussionmentioning

confidence: 99%

“…Fluorosis is one of the major public health problems affecting the rural Indian population who are dependent on ground alveolar crest, [4] root resorption, [5] hypermineralization, [6] while the soft tissue changes include gingival recession, [4] initial mineralization and globular mineralized deposits in the periodontal ligament area, [6] inhibition of Type I collagen synthesis, [7] degree of cross linking, [7] fibroblast growth inhibition, [8] lethal effects on fibroblasts [9][10][11] and morphologic changes. [8] The effect of fluorosis on dental caries is welldocumented.…”

Section: Introductionmentioning

confidence: 99%

Comparative assessment of fluorosed and nonfluorosed fibroblast attachment on fluorosed and nonfluorosed teeth after scaling and root planning and ethylenediaminetetraacetic acid root biomodification

Vandana¹,

Girotra²,

Jayashree³

et al. 2014

J Int Clin Dent Res Organ

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“…The soft tissue changes include inhibition of type I collagen synthesis, degree of cross-linking, 18 fibroblast growth inhibition, 19 lethal effects on fibroblasts, [20][21][22] and morphologic changes. 19 The occurrence of periodontitis in high water fluoride areas has shown a global variation 7,10,23,24 due to involvement of multiple risk factors in its causation.…”

mentioning

confidence: 99%

Fluorosis: Environmental Risk Factor for Periodontal Disease?

Olayanju

Borrás

Qaqish

et al. 2017

Journal of Postgraduate Medicine, Education and Research

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Aim Determination of the effect of varying fenestration technique, and simulated patch graft on outflow facility for Baerveldt tube. Materials and methods Silicone tubing similar to Baerveldt implant (AMO, Santa Ana, CA) with different fenestrations techniques was connected to a digital manometer in a closed system with a fluid-filled syringe on a stand to adjust pressure. The venting slits included: (A) 4 piercings with 7–0 TG140-8 needle; (B) a 2-mm slit with a 15° blade; (C) 4 piercings with a 15° blade; (D) 9–0 Nylon on CS140-6 needle with suture stenting the fenestration. Results For pressures of 10, 20, 30, 40 mm Hg in groups A to D, the average outflow facility (mL/min/mm Hg) were group A: 0.11, 0.20, 0.28, 0.40; group B: 0.30, 0.69, 0.98, 0.93; group C: 0.73, 0.80, 0.81, 0.88; group D: 0.58, 0.65, 0.80, 0.87. For external compression with 10 gram weights at pressures of 10, 20, 30, 40 mm Hg, outflow were group A: 0.0, 0.18, 0.20, 0.53; group B: 0.75, 0.70, 0.97, 1.21. Group C: 0.18, 0.03, 0.57, 0.04. Group D: 0.73, 0.90, 1.13, 0.91. Conclusion Effectivity of venting slits in maintaining adequate IOP in the early postoperative period for non-valved glaucoma implant is variable, multifactorial and largely intraocular pressure (IOP) dependent. Clinical significance This study explores methods of producing fenestration and the effects on outflow at different pressures in an attempt to determine which fenestration technique has more reproducible results that can be made applicable in clinical practice. This is also the first study to evaluate the effect of external pressures similar to scleral patch graft on the tube fenestrations. How to cite this article Olayanju J, Borras T, Qaqish B, Fleischman D. Outflow Facility in Tube Shunt Fenestration. J Curr Glaucoma Pract 2018;12(3):113-118.

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Effect of Sodium Fluoride on Growth of Human Diploid Cells in Culture

Cited by 17 publications

References 9 publications

Fluorosed fibroblast attachment on fluorosed and nonfluorosed teeth after SRP and EDTA root biomodification

Fluorosed fibroblast attachment on fluorosed and nonfluorosed teeth after SRP and EDTA root biomodification

Comparative assessment of fluorosed and nonfluorosed fibroblast attachment on fluorosed and nonfluorosed teeth after scaling and root planning and ethylenediaminetetraacetic acid root biomodification

Fluorosis: Environmental Risk Factor for Periodontal Disease?

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