Effect of smear layer deproteinization with chemo-mechanical caries removal agents on sealing performances of self-etch adhesives

Kusumasari, Citra; Abdou, Ahmed; Tichý, Antonín; Hatayama, Takashi; Hosaka, Keiichi; Foxton, Richard M.; Wada, Takahiro; Sumi, Yasunori; Nakajima, Masatoshi; Tagami, Junji

doi:10.1016/j.jdent.2020.103300

Cited by 14 publications

(18 citation statements)

References 33 publications

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“…The application of the solutions for 5 s did not have a significant effect on the amideto-phosphate ratios on the smear layer-covered dentin, but their application for 15 s and 30 s significantly decreased the ratios regardless of the wash-out time. However, the smear layer was still observed using SEM after the pretreatments, indicating that the dissolution of the organic phase occurred only within the smear layer 6,8,32,33) and that its inorganic phase remained on the bonding surface. This finding agrees with previous studies where no morphological alterations of the smear layer-covered sound dentin surface were observed after the deproteinizing pretreatment 6,8) .…”

Section: Discussionmentioning

confidence: 99%

“…The deproteinizing effect was assessed by comparing the collagen-to-apatite ratio before and after pretreatment. The amide I band at 1,643 cm −1 (stretching vibrations of C=O) was selected as representative of collagen, and the ν 3 band at 1,026 cm −1 (stretching vibrations of P-O) represented apatite 5,9,32) . The amide-to-phosphate ratios were statistically analyzed using a three-way repeated…”

Section: Atr-ftir Spectroscopymentioning

confidence: 99%

“…The specimens were fixed using 2.5% glutaraldehyde for 2 h at 4°C, followed by a 0.1% osmium solution for 2 h at 4°C, and serially dehydrated with ethanol as follows: 50%, 70% and 80% ethanol for 25 min each at 4°C, then 90% and 95% ethanol for 25 min each at room temperature, and finally 100% twice for 25 min. After immersion in hexamethyldisilazane (HMDS) for 10 min, the specimens were dried in a desiccator at room temperature for 24 h 32) . They were then sputter-coated with gold and observed using the JSM-IT100 SEM at a magnification of 5,000×.…”

Section: Semmentioning

confidence: 99%

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Smear layer deproteinization with NaOCl and HOCl: Do application/wash-out times affect dentin bonding of one-step self-etch adhesives?

et al. 2022

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This study investigated the influence of application/wash-out times of sodium hypochlorite (NaOCl) and hypochlorous acid (HOCl) on dentin bond strength of one-step self-etch adhesives (1-SEAs). Human coronal dentin discs with a standardized smear layer were pretreated with 6% NaOCl or 100 ppm HOCl for 5 s, 15 s, or 30 s, and washed out with water for 5 s, 15 s or 30 s with or without the application of Clearfil DC Activator (CDA). No pretreatment was used as a control. The discs were bonded with a 1-SEA (Bond Force II or Clearfil Universal Bond Quick) and microtensile bond strength (µTBS) was measured after 24 h. Pretreatment with NaOCl for 15 s and 30 s significantly decreased µTBS (p<0.05), irrespective of wash-out time. The application of CDA recovered µTBS but did not outperform the control group. Conversely, pretreatment with HOCl for 15 s and 30 s followed by 30 s wash-out time significantly increased µTBS of 1-SEAs (p<0.05), regardless of CDA application.

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Section: Discussionmentioning

confidence: 99%

Section: Atr-ftir Spectroscopymentioning

confidence: 99%

Section: Semmentioning

confidence: 99%

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Smear layer deproteinization with NaOCl and HOCl: Do application/wash-out times affect dentin bonding of one-step self-etch adhesives?

et al. 2022

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“…With smear-layer deproteinization, agents such as NaOCl or hypochlorous acid (HOCl) are used as a surface pre-treatment to remove the organic portion of the smear layer, that generally contains not only organic residues but also residual hydroxyapatite, rotatory instrument debris and bacteria 25 . This was not the aim of the present review, as such technique is used to improve and strengthen subsequent hybridization of self-etch adhesives to dentin 50 , 51 .…”

Section: Discussionmentioning

confidence: 99%

Collagen-depletion strategies in dentin as alternatives to the hybrid layer concept and their effect on bond strength: a systematic review

Delgado

Costa

Polido

et al. 2022

Sci Rep

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Strategies aiming to improve the longevity of resin–dentin adhesive interface developed so far have only been able to retard the problem. Different approaches are thus needed. The objective of this review was to determine whether the use of collagen-depletion strategies after acid-etching procedures may improve the bond strength of resin-based materials to dentin. A systematic review was planned following 2021 PRISMA statement guidelines, with a search strategy performed in five electronic databases: PubMed/Medline, Scopus, EMBASE, SciELO and IADR Abstract Archive (last search: 17/01/2022). Inclusion criteria encompassed studies which evaluated a collagen-depletion strategy in acid-etched human dentin and tensile/shear bond strength tests. Risk of bias assessment was carried out by two reviewers, working independently on an adapted five-domain risk of bias (RoB) checklist for laboratory studies. Results were synthesized qualitatively, as a meta-analysis was not possible due to limited number of studies and their RoB. A total of eight studies were eligible for inclusion in the systematic review after inclusion/exclusion criteria application. Out of these, two evaluated the effect of using NaOCl followed by an antioxidant, and the remaining six evaluated different enzymatic treatments (bromelain, chondroitinase ABC, papain, and trypsin). None of the studies reported a decrease of bond strength when a collagen-depletion strategy was used, in comparison to traditional hybrid layers (control). All enzymatic treatment studies which respected the inclusion criteria improved the bond strength to dentin. Some specific collagen-depletion strategies seem to play a favorable role in improving immediate bond strengths to dentin. Further research with sound methodology is required to consolidate these findings, since limitations in RoB and a low number of studies were found. The assessment of further proteolytic agents and long-term outcomes is also required.

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“…Group 1 (red) mainly focused on the effects of chemo-mechanical caries removal methods on the properties of the smear layer [16,[23][24][25] and how these parameters influenced the adhesion [16,[26][27][28][29][30] and the bond strength of dental adhesives [2,16,27,[31][32][33][34][35]. Papacárie, a papain-based gel, was used in several studies to compare the efficacy of different CMCR methods [12,16,23,30,31,[36][37][38][39][40][41][42][43][44][45][46][47]. Sodium hypochlorite was also tested in comparison with other CMCR agents, especially for endodontic use [26,46,[48][49][50].…”

Section: Keyword Analysismentioning

confidence: 99%

A Bibliometric Analysis (2010–2020) of the Dental Scientific Literature on Chemo-Mechanical Methods of Caries Removal Using Carisolv and BRIX3000

et al. 2022

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Reports in the literature have proposed and analyzed several minimally invasive techniques for caries removal in recent decades. In light of recent events surrounding the ongoing epidemiological context, concerns have been raised regarding the generation of aerosols during dental procedures. The aim of our research was to provide an overview of the scientific literature on the topic of chemo-mechanical caries removal (CMCR) methods, focusing on two products (Carisolv, BRIX3000), commercially available in Europe. A bibliometric analysis was used to investigate the scientific articles included in Web of Science (WoS) Core Collection database, published from January 2010 to December 2020. We analyzed the co-occurrence of all keywords (Author Keywords and the KeyWords Plus section), co-authorship and co-citation, using the free software VOSviewer. Our bibliometric analysis revealed a worldwide interest in the subject of chemo-mechanical methods of caries removal, which has transcended the area of pediatric dentistry. The analyzed studies have been conducted mainly in high-income countries that have developed sanitary policies regarding prevention and early treatment of carious lesions as a health priority.

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Effect of smear layer deproteinization with chemo-mechanical caries removal agents on sealing performances of self-etch adhesives

Cited by 14 publications

References 33 publications

Smear layer deproteinization with NaOCl and HOCl: Do application/wash-out times affect dentin bonding of one-step self-etch adhesives?

Smear layer deproteinization with NaOCl and HOCl: Do application/wash-out times affect dentin bonding of one-step self-etch adhesives?

Collagen-depletion strategies in dentin as alternatives to the hybrid layer concept and their effect on bond strength: a systematic review

A Bibliometric Analysis (2010–2020) of the Dental Scientific Literature on Chemo-Mechanical Methods of Caries Removal Using Carisolv and BRIX3000

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