Effect of simulated weightlessness on osteoprogenitor cell number and proliferation in young and adult rats

Basso, N.; Bellows, C. G.; Heersche, J. N. M.

doi:10.1016/j.bone.2004.09.016

Cited by 49 publications

(33 citation statements)

References 33 publications

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“…Microgravity, as one of the most essential elements of space flight, can induce extensive physiological changes in human (Basso et al 2005). Our work concurred with previous observation that the shape of spindle stem cells derived from rat BMSCs turned round after simulated microgravity (SMG) (Chen et al 2011).…”

Section: Introductionsupporting

confidence: 91%

The simulated microgravity enhances multipotential differentiation capacity of bone marrow mesenchymal stem cells

Wang

Zhang

et al. 2013

Cytotechnology

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Multi-differentiation capability is an essential characteristic of bone marrow mesenchymal stem cells (BMSCs). Method on obtaining higherquality stem cells with an improved differentiation potential has gained significant attention for the treatment of clinical diseases and developmental biology. In our study, we investigated the multipotential differentiation capacity of BMSCs under simulated microgravity (SMG) condition. F-actin staining found that cytoskeleton took on a time-dependent change under SMG condition, which caused spindle to round morphological change of the cultured cells. Quantitative PCR and Western Blotting showed the pluripotency marker OCT4 was up-regulated in the SMG condition especially after SMG of 72 h, which we observed would be the most appropriate SMG duration for enhancing pluripotency of BMSCs. After dividing BMSCs into normal gravity (NG) group and SMG group, we induced them respectively in endothelium oriented, adipogenic and neuronal induction media. Immunostaining and Western Blotting found that endothelium oriented differentiated BMSCs expressed higher VWF and CD31 in the SMG group than in the NG group. The neuron-like cells derived from BMSCs in the SMG group also expressed higher level of MAP2 and NF-H. Furthermore, the quantity of induced adipocytes increased in the SMG group compared to the NG group shown by Oil Red O staining, The expression of PPARc2 increased significantly under SMG condition. Therefore, we demonstrated that SMG could promote BMSCs to differentiate into many kinds of cells and predicted that enhanced multi-potential differentiation capacity response in BMSCs following Electronic supplementary material The online version of this article

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Section: Introductionsupporting

confidence: 91%

The simulated microgravity enhances multipotential differentiation capacity of bone marrow mesenchymal stem cells

Wang

Zhang

et al. 2013

Cytotechnology

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“…In the current work, a morphological investigation involving the porosity assessment was performed by using scanning electron microscopy, gravimetric measurements and mercury intrusion porosimetry, whereas the in vitro degradation kinetics in different aqueous media was investigated by the combined use of vibrational micro-Raman spectroscopy and thermogravimetry (TGA), already proved suitable for this purpose [22][23][24][25][26]. Finally, the interactions of human cells, marrow stromal cells (MSC) and osteoblasts (HOB), respectively, with the scaffold have been studied to assess the role of chemistry and scaffold morphology on bone cell behaviour.…”

Section: Introductionmentioning

confidence: 99%

Polylactic acid fibre-reinforced polycaprolactone scaffolds for bone tissue engineering

et al. 2008

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“…In femoral cell populations of adult rats, HU decreased CFU-O and CFU-AP by 16.6% and 36.6%, respectively. No changes in CFU-O or CFU-AP were detected in cell populations from the humerus [17]. This suggests that the decrease of osteogenesis in young rats resulting from HU likely plays an important role in the loss of bone mass.…”

Section: Rna Extraction and Rt-pcrmentioning

confidence: 69%

Effects of Hindlimb Unloading on Ex Vivo Growth and Osteogenic/Adipogenic Potentials of Bone Marrow-Derived Mesenchymal Stem Cells in Rats

Pan

Yang

Guo

et al. 2008

Stem Cells and Development

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Effect of simulated weightlessness on osteoprogenitor cell number and proliferation in young and adult rats

Cited by 49 publications

References 33 publications

The simulated microgravity enhances multipotential differentiation capacity of bone marrow mesenchymal stem cells

The simulated microgravity enhances multipotential differentiation capacity of bone marrow mesenchymal stem cells

Polylactic acid fibre-reinforced polycaprolactone scaffolds for bone tissue engineering

Effects of Hindlimb Unloading on Ex Vivo Growth and Osteogenic/Adipogenic Potentials of Bone Marrow-Derived Mesenchymal Stem Cells in Rats

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